26 June, 2012 -
The Dorsett Hotel, Kuala Lumpur, Malaysia
Price: US$ 795
A one-day short course in the practical application of reversed-phase high-performance liquid chromatography (RP-HPLC) methods in routine analysis
Who should take this course?
This course is designed for laboratory workers who are responsible for running reversed-phase HPLC methods on a day-to-day basis. No prior experience is needed, although those with some laboratory experience will certainly benefit more than those with no experience at all. New workers in the lab, scientists from other disciplines that have to use HPLC as a tool, laboratory managers, and quality assurance staff will all benefit from this course.
What does it cover?
This one-day class focuses on existing reversed-phase HPLC methods and how to keep them running reliably. It includes a look at how the hardware operates, how reversed- and normal-phase separations work, and troubleshooting separation problems.
• How HPLC equipment works – the parts and what they do
• What is so important about the HPLC column and how it works
• Why is system suitability testing so important
• Reversed-phase HPLC – why it forms the foundation of most methods
• Which variables affect the appearance of the chromatogram
• Troubleshooting problems with the method – isolation and correction
• What is validation and why do we do it
• Quality issues that help improve the results
What will I get from this course?
You will gain an understanding about what happens “behind the scenes” in an HPLC method. Armed with this understanding of the equipment and separations, you will be more able to keep methods working reliably and getting them back to operation when problems occur. You will understand some of the quality-related practices that can be used to improve the reliability and usefulness of the data you gather.. You will receive a printed copy of the slides included in this course plus notes on the most important points for each slide. The instructor is available to answer student questions during the class.
Course Outline
Section 1. Introduction and HPLC Basics
System suitability
Basic HPLC processes
Key chromatographic measurements
Section 2. HPLC Equipment
Reservoirs & mobile phases
Pumps & mixing
Tubing & fittings
Injectors & autosamplers
Detectors
Section 3. HPLC Columns
The underlying silica
Bonded phases
Care & treatment of columns
Section 4. Reversed-Phase HPLC
Retention mechanism
The role of the solvent
The role of the bonded phase
Other variables: pH, temperature, ion pairing
Section 5.Troubleshooting HPLC Separations
Why columns die
Physical problems with the column
Chemical problems with the column
Non-column problems
Section 6. Quality Issues
Validation
Regulatory issues
System suitability
Improving precision
Method adjustment vs. method change
Please email any questions to jackie.tan@sepscience.com
To register for this workshop simply complete the form below:
10% Discount when multiple courses are booked together.