5 July, 2012 - Sydney, Australia
Medina Grand Sydney
511 Kent Street, Sydney NSW 2000, Australia
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Sponsored by: |
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Price: US$795
An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices.
Who should take this course?
This course is designed to provide basic knowledge and practical applications of LC-MS for liquid chromatographers. Most of the examples emphasize the most popular use of LC-MS/MS today: the analysis of drugs in metabolites in biological extracts. Although no previous experience with HPLC is necessary, the course will be especially valuable to those with some chromatography experience.
What does it cover?
This is a one-day version condensed from the normal two-day class.
LC-MS for Chromatographers is a course explaining the principles of mass spectrometric detection, the design and operation of mass spectrometers, and the interfacing of the MS to the liquid chromatograph to obtain both qualitative and quantitative data. This course covers:
• MS operation, including the operation of the most popular LC-MS interfaces
• How a quadrupole mass filter works
• MS calibration and optimization
• Ion production, fragmentation and detection
• Operation in MS, MS-MS, and MS-MS-MS modes
• Structure determination by product ion analysis
What will I get from this course?
You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the molecular ion, you will have a solid grounding on which to further build your LC-MS expertise. You will receive a printed copy of the slides included in this course plus notes on the most important points for each slide. The instructor is available to answer student questions during the class.
Course Outline
Introduction and Overview of LC-MS
Principles of Quadrupole Mass Analyzers
Basics of Quadrupole Instrumentation
The (pseudo) Molecular Ion
Calibration and Tuning
Qualitative Analysis
Quantitative Analysis
Developing the MS Method
Developing the LC Method
Summary
Please email any questions to jackie.tan@sepscience.com
Please email any questions to jackie.tan@sepscience.com
To register for this workshop simply complete the form below:
10% Discount when multiple courses are booked together.