Price: US$997
Who should take this course?
If you use chromatography on the job and would like a better understanding of how it works, "HPLC Basics, Equipment, and Troubleshooting" is the course for you. It's designed for chemists and biochemists who use HPLC as a regular part of their jobs, but technicians with some HPLC experience will also find the course valuable. No previous HPLC training is assumed; however, much of the course will appeal to the experienced chemists who want a firm grounding in the basics of HPLC.
What does it cover?
"HPLC Basics, Equipment, and Troubleshooting" explains chromatography in practical terms from the ground up. Here's what you'll learn in this three-day course:
• The basic principles of HPLC
• The five fundamental HPLC modes
• The easy and logical way to adjust experimental variables to get a good separation
• The best approach to use for various chromatographic applications
• How to maximize column life
• Special techniques such as gradient elution, quantitation, and sample pretreatment
• The operating principles of each module in an HPLC system
• Proven techniques for systematic problem-solving and instrument maintenance
• The most effective, timesaving, money saving approaches to preventing common problems.
• And much, much more
What will I get from this course?
You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient. What you learn will demystify your instrument. You'll find that all of the perplexing and frustrating problems your experience have simple and logical solutions. And, better yet, you'll learn that most of these problems are preventable. The techniques you learn from this course will make you more effective at work and save you big headaches! If you've never taken a formal course or if you need a refresher, it's time to learn HPLC the right way. Students tell us that this "extremely practical course" is a must for everyone who uses HPLC.
Course Outline
Day 1
• Registration
• HPLC Basics• Mobile Phase Reservoirs• Pumps and Mixing• Tubing and FittingsLunch• Sample Injectors & Autosamplers• HPLC Detectors• Conclusions/Questions
Day 2
• HPLC Columns• Reversed-Phase HPLC• pH Effects and Ion-Pair Lunch• Gradient Elution• Column Problems and Fixes• Tailing Bands• Quantitative HPLC• Conclusions/Questions
Day 3
• Separation Chemistry Problems
• Sample Preparation
• Ion-Exchange HPLC
• Normal-Phase HPLC
• Size-Exclusion HPLC
Lunch
• Bioseparations by HPLC
• Case Studies
• Review
• Conclusion
About Live on the Web Format
Live on the Web courses run from 8:00am to 10:30am Pacific time. There is a short (5-10 minute) coffee break in the middle. HPLC Basics, Equipment, and Troubleshooting run on Tuesday and Thursday mornings. Because the courses are live, the exact timing of topics varies somewhat from one presentation to another, depending on attendee questions. Each Course is a total of 6 sessions.
Dr John Dolan has published over 100 scientific articles with emphasis on method development techniques, gradient elution, and column selectivity. He is the author (with Lloyd Snyder) of three books, Troubleshooting LC Systems, High-Performance Gradient Elution, and the 3rd edition of Introduction to Modern Liquid Chromatography (also with Jack Kirkland) – often referred to as the Bible of HPLC.
Please email any questions to jackie.tan@sepscience.com.
To register for this workshop simply complete the form below:
Additional registrants from the same company are half-price. Please contact Jackie.tan@sepscience.com to register group bookings.