April 3 - May 17, 2012 - LIVE ON THE WEB
LC/MS: Fundamentals and Applications is a comprehensive overview course that provides attendees with relevant, practical approaches that can be immediately applied in the lab. The theory of LC/MS interfaces (electrospray, atmospheric pressure chemical ionization, and atmospheric pressure photoionization), is presented to the extent necessary to understand operation of these instruments but the real focus of this class is improving your ability to use LC/MS on a daily basis. This course is offered as a three-day workshop.
Who Should Attend
Researchers, practitioners, technicians, and others who are currently using LC, LC/MS, or plan to use LC/MS in the future, and those dealing with data produced by LC/MS. This course is designed to provide a practical overview. Chromatographers just embarking on the technique will gain insight on how to select the appropriate instrument for different applications, and those currently using LC/MS and its data will develop an appreciation for, and an understanding of, the complexities of the data generated.
How You'll Benefit from This Course:• Understand the operational fundamentals of the commercially available LC/MS interfaces. • Learn a step-by-step approach to developing effective LC/MS methods and how this approach differs from one interface type to another.• Receive an overview of LC/MS applications in pharmaceutical, chemical, and biotechnology industries plus information on forensic and toxicological applications • Learn how to modify existing HPLC methods for transfer to LC/MS. • Understand the data generated by your LC/MS instrument for qualitative evaluation of unknown samples and quantitative analysis.
Course Topics:• Introduction to LC/MS: overview and historical perspective • Review of atoms, isotopes, molecules and ions: how to think about these topics in order to better understand mass spectrometry• LC/MS Interfaces: historical interfaces, atmospheric pressure chemical ionization, electrospray and atmospheric pressure photoionization interfaces• Optimization of LC/MS methods: how mobile phase and matrix components can affect results; methods for determining and fixing optimization problems; how electrospray method development differs from APCI • Handling the data from LC/MS: both qualitative (spectral interpretation) and quantitative use of MS data is discussed• Applications review: drugs and metabolites, pharmaceuticals, environmental applications, natural products, protein/peptide analysis are presented• Bibliography of LC/MS; glossary of correct and incorrect terms
About the Instructors
Fred Klink is a trainer and consultant to the pharmaceutical, biotech, and chemical industries as well as law enforcement and other government laboratories. Fred’s speciality is HPLC, LC/MS, and solid-phase extraction technologies. Fred received a degree in biochemistry from North western University and completed graduate studies and an internship in forensic chemistry at the University of Illinois. Fred is the author of several journal articles and book chapters including the LC/MS entry in the Wiley Encyclopedia of Analytical Chemistry. He is a member of the American Chemical Society and American Society for Mass Spectrometry.
O. David Sparkman is currently an Adjunct Professor of Chemistry at the University of the Pacific in Stockton, California; Consultant to the National Institute of Standards and Technology Mass Spectrometry Data Center; President of ChemUserWorld.com; and a former American Chemical Society Instructor and American Society for Mass Spectrometry Member-at-large for Education. Over the past 28 years, he has developed and taught five different ACS courses in mass spectrometry. He is the author of Mass Spectrometry Desk Reference.
Here's What Past Participants Have to Say About This Course:
"I was very pleased with the course. It fulfilled my goal of achieving a basic undderstanding LC/MS and GC/MS, which will be helpful in future method reviews and possible LC/MS operation. I also very much liked the comments for each slide. GREAT COURSE!" —Elizabeth Flynt, Chemist, U.S. EPA
" Perfect course, Thank you! It was exactly what I needed." —Ray Miller, Chemist, New Orleans, LA, USA
Please email any questions to jackie.tan@sepscience.com.
To register for the course simply complete the form below: