• When it is appropriate to use gradient HPLC
• Similarities between gradient and isocratic methods
• Differences between gradient and isocratic methods
• Hardware issues relating to dwell volume
• Hardware issues relating to baselines

John W. Dolan and Tom Jupille, LC Resources, present Session 5 of the "HPLC Troubleshooting and Preventive Maintenance: The Keys to Fixing Problems Quickly" course. This 65-minute online learning seminar covers HPLC detectors. Covered in the course are:

• Measuring Peak Area
• Calibration Curves 
• Reproducibility

• Problem Isolation
• Important Measurements 
• Tricks of the Trade
• General Priniples

• Reservoir and Degassing
• Pumps
• Autosamplers

• Plugging the Frit or Column
• Mechanical Shock
• Whens the Column is not the Problem
• Adsorbed Sample Impurities
• Chemical Attack on the Silica or Bonded Phase

Tom Jupille and John W. Dolan, LC Resources, present Session 4 of the "HPLC Troubleshooting and Preventive Maintenance: The Keys to Fixing Problems Quickly" course. This 70-minute online learning seminar covers chemical problems that occur withe the use of HPLC columns. Covered in the course are:

Tom Jupille and John W. Dolan, LC Resources, present Session 5 of the "HPLC Troubleshooting and Preventive Maintenance: The Keys to Fixing Problems Quickly" course. This 55-minute online learning seminar covers HPLC detectors. Covered in the course are:

• Overview of LC-MS and LC-MS/MS 
• Quadrupole physics 
• Instrumentation 
• Ion formation 
• Elements of LC-MS/MS analysis

• Overview of HPLC qualification 
• Pump and detector checks 
• Online mixing checks 
• Chromatographic checks