Fundamentals of Sample Preparation for Chromatography
31 Oct 2017 09:00 - 17:00
When: 31 October, 2017
Presenter: Ronald E. Majors
Taught by Dr Ronald E. Majors, who has written and lectured on sample preparation and chromatographic technology for over 30 years, the short course will provide an overview of important sample preparation techniques used for the analysis of gas-, liquid-, solid– and semi-solid matrices.
The approach will be to discuss basic principles of common techniques such as Liquid-Liquid Extraction (LLE), Solid-Phase Extraction (SPE), Solid-Phase Microextraction (SPME) as well as more advanced technologies such as pressurized-fluid extraction, QuEChERS, supported-liquid extraction, enhanced matrix removal (EMR) and membrane-based extraction procedures.
The course will be practically oriented with a minimum of theory and a maximum of examples and “how-to-do’s”. Discussions on proper sampling approaches, size-exclusion chromatography as a sample prep technique and sample prep for bioanalysis will be included in the coverage. A brief discussion on automation of sample preparation to improve throughput and reproducibility and to relieve the manual fatigue when encountering large numbers of samples will round out the short course.
Besides course notes, a 360-page book on sample preparation fundamentals for chromatography written by Dr Majors will be provided for each registrant.
Key Learning Outcomes
The attendee should leave the course:
- With a thorough understanding of the basic principles of many different modern sample prep techniques.
- With a knowledge of the application of sample prep techniques as applied to difficult pharmaceutical, chemical, biological, food and environmental matrices.
- Knowing about newer techniques for sample preparation that are faster, provide lower detection limits and better recovery and require less manual labor.
- With an understanding of proper sampling methods so that the analytical process applied afterwards can provide optimum accuracy and reproducibility.
- With a knowledge about approaches to automation for high-throughput sample prep.
Since 1990, Ronald E. Majors has been working in the area of sample preparation and chromatography column technology. He is the author of over 300 publications in HPLC, GC, sample preparation and surface chemistry. Dr Majors has served as Chairman of HPLC ‘86 and Anabiotec ‘90 and as a member of the Instrumentation Advisory Board of Analytical Chemistry. His expertise covers sample preparation, especially solid-phase extraction and automation, and HPLC and GC column technology. Dr Majors was the 1994 recipient of the Merit Award in Chromatography from the Chicago Discussion Group, the 1998 Award for Distinguished Contributions in Separation Science sponsored by the California Separations Society and, in 2000, the “Salutes to Excellence” Award from the North Jersey Chromatography Discussion Group, the 2006 Palmer Award from the Minnesota Chromatography Forum and the Chromatographic Society’s 2007 Martin Gold Medal from the United Kingdom, named for the Nobel Prize winner, A.J.P. Martin.