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A Novel 75 cm Column Size Gives Increased Resolution and Better Sequence Coverage

The separation of complex peptide mixtures and their subsequent identification is crucial in the field of bottom-up proteomics. The increased peak capacity of the 75 cm column leads to enhanced quality of the separation, even when using similar gradients. Using even longer gradients can further improves results.
The use of the Thermo Scientific™ EASY-Spray™ C18, 75 cm column offers improved peak capacity and higher sensitivity allowing enhanced sequence coverage, which is critical for better protein identification.

 

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