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Fast, Accurate LC-MS/MS Analysis of the C3 Epimer of 25-Hydroxyvitamin D on Raptor FluoroPhenyl Columns

Vitamin D analysis has increased dramatically in clinical practice due to its association with multiple human diseases including cancer, rheumatoid arthritis, and diabetes. It also has an important role in calcium and phosphorous absorption in relation to bone strength, so there is growing concern about the prevalence of vitamin D deficiency worldwide. Accurate LC analysis of vitamin D epimers is critical for quantitative determination of the bioactive forms of vitamin D.


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