Previously we went through the fundamentals, the separation medium BGE, the capillary and injection in capillary electrophoresis. In this issue of CE Solutions we will reach the end of the system, the detector. We will discuss the most popular detection principles in use in industry and CE-related good working practice.
In this issue we look at the importance of injection settings for precision. We will also have a first look at sample stacking and see that by carefully selecting our sample solvent we can gain sensitivity in our applications.
In previous issues we saw the importance of a controlled, constant electro-osmotic flow (EOF) for proper precision. In this issue we will therefore look into the different strategies for capillary conditioning, as a good conditioning procedure is crucial to a stable EOF.
In this issue of CE Solutions we will focus on the capillary as used for CE. We will look at the typical choices of length and diameter for method development. We will also look at coated capillaries and discuss both permanent coatings and adsorbed coatings.
The selection of the proper background electrolyte (BGE) is key to a successful CE method. In this second issue of our series on CE Solutions we first look into the choices for BGE based on our analyte properties. We will look into the effects of pH on analyte charge and discuss opportunities to induce charge on neutral analytes.
This article is the first in a new series about capillary electrophoresis (CE), aims to be a low-threshold, practical and pragmatic aid for that implementation process by focusing on good CE working practices and troubleshooting.