Back-to-Basics: Selectivity

This technical article considers some factors affecting the chromatographic selectivity.

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Written byJohn Dolan

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This technical article considers the factors affecting chromatographic selectivity.

In this article the topic is chromatographic selectivity. Selectivity is the ability of an HPLC method to separate two analytes from each other. Selectivity usually is abbreviated with the Greek letter α, and is calculated as: α = k₂ / k₁ where k₁ and k₂ are the retention factors, k, of the first and second peaks of a peak pair.

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Meet the Author(s):

John Dolan
John Dolan is considered to be one of the world’s top experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years, in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.
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