Overview
A deep and comprehensive understanding of the phosphoproteome is critical to gain insights into cellular regulation and disease mechanisms, such as those involved in cancer, autoimmune, and metabolic disorders. Mass spectrometry analysis enables a deep and comprehensive mechanism of inquiry into the phosphoproteome, but is often stymied by issues with high sample input requirements, low PTM stoichiometry over a high dynamic range of the total proteome, difficult sample preparation, highly labile modifications, poor ionization, and irreproducibility. Addressing these challenges involves optimizing sample preparation protocols, utilizing workflows that enrich for the phosphoproteome with high specificity, and applying application and acquisition strategies specific to your research goals and sample type.
By viewing this presentation, you will discover:
- Strategies for successful sample preparation prior to phosphoenrichment
- Phosphoenrichment workflow tips and tricks for highest identifications, phosphospecificity, reproducibility and confident localization
- Upstream and downstream workflow options for phosphoenrichment experiments
- Commercially available options for phosphoenrichment and their comparative performance on multiple mass spectrometry instrumentation platforms
- Automation capabilities on Thermo Scientific™ KingFisher™ platforms
- Acquisition strategies for label-free and TMT™- or Thermo Scientific™ TMT™ Pro-labeled phosphoproteome-enriched samples
- Phosphoenrichment considerations in various biologically relevant sample types for highest confidently localized and reproducible identifications
Watch the presentation today to overcome your analytical bottlenecks and unlock deeper insights into the phosphoproteome.
What you need to know:
Format: On demand
Duration: 45 minutes
