This technical article discusses whether high-pressure mixing or low-pressure mixing are better.
In this technical article, what is meant by high- and low-pressure mixing is discussed. High-pressure mixing systems are usually are limited to two solvents, each of which is pumped by a separate pump into the mixer, which is located on the high-pressure side of the pump. The mobile phase mixture is controlled by the relative flow rate of the two pumps. The flow rate is determined by the combined flow rate of both pumps. Most low-pressure systems have the ability to deliver any combination of up to four different solvents. Mixing takes place on the low pressure side of the pump and a single pump is operated at a constant flow rate. In terms of routine use of an isocratic or gradient HPLC method, either high- or low-pressure mixing should produce satisfactory results. It is more a matter of what is available or what is your favorite. There is really no “better” system for routine applications.
By reading the full article, you will discover factors in choosing high- or low-pressure mixing in different analyses.
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