By attending this webinar you will:
- Understand the key factors that govern oligonucleotide retention and selectivity in HILIC.
- Explore the role of the hydrogen-bond donor capability of the mobile phase.
- Gain insight into how solvophobic interactions contribute to oligonucleotide retention.
- Discover a more systematic approach to HILIC method development and optimization.
Event Overview
HILIC is increasingly used for oligonucleotide analysis, but its retention mechanisms remain far less intuitive than those of reversed-phase chromatography. In this webinar, we will explore what really drives oligonucleotide retention and selectivity in HILIC, from the hydrogen-bond donor capability of the mobile phase to solvophobic effects. Moving beyond the classical partitioning model toward the Protic Enhanced Polar and Solvophobic Interaction (PEPSI) mechanism will provide a more rational framework for HILIC method development and optimization.
What you need to know:
Date: 29 September, 2026
Start time: 7 am PDT (Los Angeles) / 9 am CDT (Chicago) / 10 am EDT (New York) / 3 pm BST (London) / 4 pm CEST (Paris/Berlin)
Duration: Approximately 40 minutes
