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This technical article looks at several ways to estimate column dead-time.
In Back-to-Basics #1 we looked at the retention factor, k, and how to calculate or estimate it. In order to perform either of these processes, we need to know the column dead time, t0. If we are using a UV detector and a “real” sample, usually there is an obvious disturbance in the baseline.
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John Dolan is considered to be one of the world’s top experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years, in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.