By viewing this presentation, you will:
- Learn about the application and advantages of IP-HILIC-MS and acrylamide-based monoliths for the characterization of diverse biomacromolecules
- Discover how HILIC-MS technologies promise to enhance our understanding of complex biomolecules
Event Overview
The analysis of intact biomacromolecules is critical for understanding structure, modifications, and biological function—but conventional reversed-phase liquid chromatography (RPLC-MS) often falls short when it comes to separating closely related variants. In contrast, hydrophilic interaction chromatography (HILIC) offers complementary selectivity, making it a powerful alternative for resolving modifications and isomers that RPLC-MS cannot distinguish.
This presentation focuses on the development of HILIC for biomacromolecule characterization. It highlights recent advances in ion-pair HILIC-MS and introduces a novel polymeric stationary phase for (IP)HILIC-MS. This poly(acrylamide-co-N,N-methylenebisacrylamide) monolithic stationary phase is designed to reduce unwanted secondary interactions and provides controlled morphology, which enhances separation capabilities compared to traditional silica-based materials.
Through real-world applications, including therapeutic oligonucleotides and intact monoclonal antibodies, learn how ion-pair HILIC-MS offers a powerful alternative to RPLC for biomacromolecule analysis. Registrants will see how this approach enables unprecedented resolution of impurities and glycoforms. Watch and explore how the potential of HILIC-MS technologies is pushing the boundaries of intact biomacromolecule characterization and opening new possibilities for biopharmaceutical analysis and quality control.
What you need to know:
Format: On demand
Duration: 37 minutes
