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Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for LC analysis users of chromatography, mass spectrometry, sample preparation and related analytical techniques.

Featured Educational Resources

Comprehensive Two-Dimensional Liquid Chromatography (LC×LC) for the Analysis of Bioactive Compounds in Foodstuffs

24 October, 2019

Luigi Mondello (University of Messina, Italy)

This webinar will help you understand the importance of foodstuffs as potential sources of bioactive molecules with beneficial effects on the human health, describe the benefits provided by comprehensive two-dimensional LC techniques, for the separation/identification of complex matrices, and demonstrate how to implement LC×LC approaches using different column combinations and instrumental set-ups.

LC-MS Calibration vs Tuning

Fundamentals

There are two procedures that are used to make sure that an HPLC equipped with a mass spectral detector (MS) is reporting proper mass information and that the system is properly set to detect the analyte(s) of interest.

Does a Guard Column Make Sense?

Fundamentals

A guard column is one of those HPLC accessories that is a bit of a mixed grill – it can be a good thing or a not-so-good thing. Let’s look at some of the different aspects of guard column use to see if they make sense for you.

Does Air Damage My Column?

Fundamentals

I accidentally ran out of solvent in an overnight run on my HPLC system. I had not activated the low-pressure limit on the pump, so the pump just pumped air for half the night. Will this ruin my column? If not, how can I get all the air out?

Pore Size vs Particle Size

Fundamentals

What is the relationship between pore size and particle size for an HPLC packing material?

Internal Standards: How Does It Work?

Fundamentals

In this article, we’ll look at the use of internal standards in HPLC and LC-MS applications, and in particular the topic of this discussion is how an internal standard is used in quantitative analysis.

What Makes a Good Internal Standard?

Fundamentals

In a earlier article we took a brief look at why we might decide to use an internal standard and how a calibration plot is constructed and used in HPLC analysis. This time, we’ll consider some properties that are necessary when choosing a compound to use as an internal standard.

HPLC Column Selection Guide to Help You Achieve the Best Separation

eBook, Application Notes and Online Tutorials

SHODEX

Learn from the expert, Dr Christian Hirsch from Shodex, who has compiled a comprehensive HPLC column selection guide to help you select the right HPLC column for your application and achieve the best separation out of your HPLC measurements. Apart from the eBook, do check out Shodex's latest application notes and educational online tutorials: Reversed Phase RP, HILIC, Size Exclusion chromatography SEC, Ligand Exchange LEX and Ion Chromatography IC.

The Fundamentals of HPLC, HPLC & UHPLC Troubleshooting, Advanced HPLC Method Development Using QbD and Principles of HPLC Validation

Self-paced Online training

Analytical Training Solutions

Each course on Analytical Training Solutions has been divided into a number of modules, with each module focusing on one particular aspect of the overall course. The modules have been created in a logical manner, and by starting with the first module and working through them in the order provided creates the best learning environment for the user. In addition, as each course is divided into modules it is then very simple to find a particular section again in the future; for example, if you want a recap on a particular subject.

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