Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for LC analysis users of chromatography, mass spectrometry, sample preparation and related analytical techniques.
Featured Educational Resources
[LC Tutorials] How to Improve Method Robustness through Control of Unwanted Interactions
Kim Haynes (Waters)
This course is designed to take you from beginner to advanced in controlling the interactions within your chromatographic separations. It will cover how to improve method robustness through the prevention of analyte loss, with improved confidence in the quality of your data.
[LC Tutorials] Understanding LC Column Attributes - What's Important for Your Chromatography?
Jonathan Turner (Waters)
This course is designed to take you from beginner to advanced in understanding column selection and method development. It will cover the key aspects of the LC column from the base particle to the bonded phase, and even the hardware.
Super Charge your Method Development with a Quick, Easy Universally Compatible LC and LC/MS Method
Anne Mack (Application Scientist, Agilent)
This webinar demonstrates how you can achieve enhanced performance for basic analytes under weak ionic strength mobile phase conditions, such as formic acid. A new method development tool is featured: a unique C18 bonded phase chemistry with a charged surface on 2.7 μm superficially porous particles.
Getting your LC/GC Lab Up and Running Again
Paul Altiero and Mark Sinnott (Agilent)
Regardless of why your lab may have been shut down, you need to get your LC and GC instruments back up and running quickly and safely. This presentation covers the information you need to get back to your important research including restart procedures, helpful service, items, support topics, and discussions on improving operational efficiency and capacity.
Trials and Tribulations of Fat Soluble Vitamin Analysis by UHPLC-MS/MS
Alan Edgington (Biotage, UK)
This webinar summarizes the work performed to develop a method for the analysis of fat-soluble vitamins including vitamins A (Retinol and Beta Carotene), D (25 Hydroxy D3 and 25 Hydroxy D2), E (Alpha Tocopherol) and K (Phylloquinone and Menaquinone) in human serum.
Introduction to and Practical Applications of the SCIEX Triple Quad 7500 LC-MS/MS System – QTRAP Ready
Ian Moore and Jianru Stahl-Zeng (SCIEX)
Learn how the SCIEX 7500 System enables you to attain up to 7X greater sensitivity to quantify more trace analytes at lower levels across a broad suite of sample types and workflows.
Making the Most of your LC System
Mark Powell (Applications Engineer, Agilent)
This on-demand webinar will cover the LC system overview, from solvent bottles to detector, with a focus on preventive maintenance and what to look out for to keep your systems running at optimal performance.
Ion Chromatography Solutions in the Petrochemical Industry
Iris Reber (Metrohm, Herisau, Switzerland) and Ricardo Sales Tena (BP, Castellón, Spain)
In this webinar, you will learn more about IC and discover solutions to some of the challenges petrochemical labs are faced with. Further, you will get to know various inline sample preparation techniques that will allow you to save valuable time and resources.
Comprehensive Two-Dimensional Liquid Chromatography (LC×LC) for the Analysis of Bioactive Compounds in Foodstuffs
Luigi Mondello (University of Messina, Italy)
Learn about the benefits provided by comprehensive two-dimensional LC techniques, for the separation/identification of complex food matrices, as well as how to implement LC×LC using different column combinations (e.g. NP×RP, RP×RP and HILIC×RP) and instrumental set-ups.
LC-MS Calibration vs Tuning
There are two procedures that are used to make sure that an HPLC equipped with a mass spectral detector (MS) is reporting proper mass information and that the system is properly set to detect the analyte(s) of interest.
Does a Guard Column Make Sense?
A guard column is one of those HPLC accessories that is a bit of a mixed grill – it can be a good thing or a not-so-good thing. Let’s look at some of the different aspects of guard column use to see if they make sense for you.
Does Air Damage My Column?
I accidentally ran out of solvent in an overnight run on my HPLC system. I had not activated the low-pressure limit on the pump, so the pump just pumped air for half the night. Will this ruin my column? If not, how can I get all the air out?
Pore Size vs Particle Size
What is the relationship between pore size and particle size for an HPLC packing material?
Internal Standards: How Does It Work?
In this article, we’ll look at the use of internal standards in HPLC and LC-MS applications, and in particular the topic of this discussion is how an internal standard is used in quantitative analysis.
What Makes a Good Internal Standard?
In a earlier article we took a brief look at why we might decide to use an internal standard and how a calibration plot is constructed and used in HPLC analysis. This time, we’ll consider some properties that are necessary when choosing a compound to use as an internal standard.
HPLC Column Selection Guide to Help You Achieve the Best Separation
eBook, Application Notes and Online Tutorials
Learn from the expert, Dr Christian Hirsch from Shodex, who has compiled a comprehensive HPLC column selection guide to help you select the right HPLC column for your application and achieve the best separation out of your HPLC measurements. Apart from the eBook, do check out Shodex's latest application notes and educational online tutorials: Reversed Phase RP, HILIC, Size Exclusion chromatography SEC, Ligand Exchange LEX and Ion Chromatography IC.
The Fundamentals of HPLC, HPLC & UHPLC Troubleshooting, Advanced HPLC Method Development Using QbD and Principles of HPLC Validation
Self-paced Online training
Analytical Training Solutions
Each course on Analytical Training Solutions has been divided into a number of modules, with each module focusing on one particular aspect of the overall course. The modules have been created in a logical manner, and by starting with the first module and working through them in the order provided creates the best learning environment for the user. In addition, as each course is divided into modules it is then very simple to find a particular section again in the future; for example, if you want a recap on a particular subject.
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