Maximizing Oligonucleotide Separation: Innovations in HPLC Performance

By viewing this presentation, you will learn about:

• How fused-core columns achieve sub-five-minute separations of 100-mer ssDNA with high resolution
• Method adjustments that stabilize retention under high-pH conditions
• How a 120 Å pore size supports separation of oligomers up to 60 bases
• Case studies that demonstrate practical gains in both R&D and QC labs

Event Overview

Longer oligonucleotides are pushing routine HPLC toward its limits. Co-elution increases, retention can shift at high pH, and some impurities become harder to resolve. The result: more repeats and slower turnarounds for R&D and QC.

The presentation explains how fused-core particle design shortens diffusion paths and maintains resolution under high-pH stress, even with 100-mer mixtures. It shows how the Oligonucleotide HPLC Performance Mix helps set retention benchmarks and monitor column stability over time. It also covers how size-exclusion chromatography (SEC) and anion-exchange chromatography (AEX), paired with multi-angle light scattering (MALS), confirm molecular weight and reveal impurities that primary HPLC methods may overlook.

Who should view?

R&D and QC teams running oligonucleotide separations, method development, or method transfer.

What you need to know:

Format: On Demand

Duration: 59 Mintues

Dr. Egidijus Machtejevas Lead Expert, Product & Portfolio Manager, HPLC Biomolecules, Life Science, Chemistry, Analytical Chemistry, Merck Life Science KGaA, Darmstadt, Germany Egidijus Machtejevas earned his PhD in analytical chemistry at Kaunas University of Technology and completed postdoctoral research at Mainz University in Germany. He later worked at AstraZeneca in Sweden on multidimensional systems for peptidomic analysis. Since joining Merck KGaA, Germany, Darmstadt, in 2008, he has held roles in R&D, marketing, and product management and now serves as Senior Technical Advisor in Analytical Chromatography Workflows, specializing in chromatography, proteomics, and mass spectrometry.

Dr. Andrea Krumm Commercial Product Manager ‑ Analytical Solutions, Tosoh Bioscience GmbH Separations Business Unit ‑ Europe Andrea Krumm studied biotechnology before obtaining a PhD in cancer biology. After four years as an applications specialist for optical analytical devices used in life science research, she joined Tosoh Bioscience GmbH in 2020 as a product manager for analytical solutions. In this role, she is responsible for gathering customer requirements and application areas and addressing these with new and existing products of the analytical columns line and the Tosoh MALS detector.

*The Life Science business of Merck, KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.

For further information about our names in the US, Canada, and internationally, please refer to our disclaimer at merckgroup.com.