The second part of this series focuses on the allowable adjustment in the ratio of aqueous (A) to organic (B) components of a reversed-phase mobile phase.
In the previous article (Part 1), we took an overview of the concept of method adjustment vs method change in reversed-phase HPLC, in light of the guidelines of the European Pharmacopoeia (EP) and United States Pharmacopoeia (USP). We also looked at an example of the allowed changes in mobile phase pH and buffer strength. In this article, we’ll move down the chart and look at the variable listed in Table 1.
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