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Method Adjustment vs Change Part 5: Volume, Column Temperature and Detector Wavelength

This Edition of HPLC solutions is talking about the injection volume, column temperature and detector wavelength adjustments.
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John Dolan
byJohn Dolan
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This technical article discusses the injection volume, column temperature and detector wavelength adjustments.

In the last few articles we've focused on the guidelines for method adjustment listed in the European Pharmacopoeia (EP) and United States Pharmacopoeia (USP). We looked at pH (Part 1), mobile-phase composition (Part 2), column dimensions (Part 3), and flow rate and particle size (Part 4). This week we’ll focus on the remaining variables listed in Table 1.

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Meet the Author(s):

  • John Dolan

    John Dolan

    John Dolan is considered to be one of the world’s top experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years, in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.

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