By viewing this on demand presentation, you will:
- Learn about the difficulties involved in the toxicological analysis of cannabinoids, with a focus on how the presence of isomers like Δ8-THC creates analytical complications.
- Discover solutions to several of these challenges, including complete workflows for the analysis of Δ8-THC, Δ9-THC, and metabolites in both whole blood and urine.
Event Overview
The testing of blood and urine to determine exposure to cannabis has been routine for decades. Most laboratories will include Δ9-tetrahydrocannabinol (Δ9-THC), the main psychoactive component in cannabis, as well as its two metabolites, 11-Hydroxy-Δ9-Tetrahydrocannabinol (11-OH-Δ9-THC) and 11-nor-9-carboxy-Δ9-Tetrahydrocannabinol (Δ9-THC-COOH) in their testing scopes. As more isomers of Δ9-THC become available on the market, testing becomes more complicated, and novel methods are needed to achieve isomeric resolution. One such isomer, Δ8-THC, emerged on the market after the passing of the 2018 Farm Bill that appeared to remove the isomer from the federal list of controlled substances. Δ8-THC forms its own hydroxy and carboxy metabolites, which are also isomers of 11-OH-Δ9-THC and Δ9-THC-COOH.
In this presentation, we will examine the classic challenges associated with cannabinoid analysis in biological matrices, as well as how the emergence of isomers like Δ8-THC creates further complications. The development of an LC-MS/MS method to separate Δ8-THC, Δ9-THC, and their hydroxy and carboxy metabolites in whole blood and urine will be discussed. We will cover special considerations that must be made for both matrices, such as cross-analyte interferences in whole blood and extreme isomer ratios in urine.
Who should view:
- Lab directors, scientists, and analysts interested in the toxicological analysis of cannabinoids in biological fluids.
What you need to know:
Format: On-demand
Duration: 41 minutes
Presenter
Haley Berkland, MS
LC Applications Scientist, Restek Corporation
Haley is an LC applications scientist at Restek. She attended Duquesne University, receiving her bachelor's degree in biochemistry and a master's degree in forensic science and law. As a graduate student, she performed research on the detection of drugs of abuse in vitreous humor by LC-MS/MS. Before joining Restek in 2023, Haley spent four years working as a forensic toxicologist. While in this role, she performed analysis of postmortem toxicology casework, identification of seized drug evidence, and development/validation of new assays by LC-MS/MS, GC-MS, and GC-FID.
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