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Retention Time & Column Dead Time Diagnostics

This article regards the complication cause from changes in retention time and dead-time.
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Written byJohn Dolan
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This technical article looks at diagnosing problems with our HPLC separations.

If we look at change in the chromatogram and concentrate on what happens to the retention time, tR, and the column dead time, t0, there are only four possible combinations: both t0 and tR change together, only tR changes, only t0 changes (highly unlikely), and neither variable changes (no change = no problem). Only the first two are of interest. First, let’s consider what could be the matter if both retention and the column dead-time change together. It has to be either a flow-rate-related change or a change of the column size.

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Meet the Author(s):

  • John Dolan

    John Dolan

    John Dolan is considered to be one of the world’s top experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years, in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.

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