Sample Prep Workflows for Low-Level LC-MS/MS Analysis

Discover best practices in sample prep workflows, compare LLE, SLE, and SPE techniques, and explore the future of sample preparation.

| 1 min read

byAimee Cichocki and Stephanie Marin

In the pursuit of accurate and sensitive LC-MS/MS analysis, particularly at low analyte concentrations, sample preparation is a crucial step that can make or break the success of an assay. Separation Science recently engaged in an insightful discussion with Dr. Stephanie Marin, the Global Clinical Market Development Manager at Phenomenex, to explore the intricacies of sample preparation workflows for low-level LC-MS/MS analysis.

With extensive experience in developing and validating clinical assays, Dr. Marin brings a wealth of knowledge in liquid chromatography and mass spectrometry to the conversation. In this interview, she provides an in-depth comparison of various extraction techniques, shedding light on their suitability in the context of endocrine testing.

In this interview, Dr. Marin provides insights in response to the following questions:

Could you provide a brief overview of liquid-liquid extraction (LLE), solid-liquid extraction (SLE), and solid-phase extraction (SPE)?
How do LLE, SLE, and SPE compare in terms of efficiency, recovery, and reproducibility for endocrine testing?
How do you see the future of sample prep evolving, particularly for low-level endocrine testing?

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Meet the Author(s):

Aimee Cichocki
Aimee Cichocki is the Managing Editor at Separation Science and Chromatography Forum. Aimee brings a broad range of experience in creating, editing, and formatting scientific content. With a degree in medicinal chemistry, a 10-year background in formulation chemistry, an MBA, and a diverse background in publishing, Aimee guides editorial initiatives at Separation Science and Chromatography Forum. Aimee is dedicated to ensuring the delivery of informative, reliable, and practical content to our audience of analytical scientists.
Stephanie Marin
Stephanie J. Marin, Ph.D. is the Clinical and Forensics Global Market Development Manager at Phenomenex. She received her Ph.D. in chemistry from Arizona State University. She has expertise in sample preparation, liquid chromatography, and mass spectrometry, and has over 10 years of experience developing and validating clinical assays from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr Marin has worked in LC product and applications development at Hamilton Company and Selerity Technologies, in analytical services for Rohm and Haas (now Dow) and was a supervisor at an EPA certified laboratory. Before joining Phenomenex, Stephanie was a Senior Applications Chemist at Biotage. She is the author of over 30 peer reviewed publications and book chapters and over 100 abstracts presented at national and international meetings.

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