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This technical article takes a closer look at the influence of temperature on the separation.
Ever have an HPLC method that specifies “ambient” as the column temperature? That’s a good sign of potential problems in the future for the method. We’ll get back to the interpretation of “ambient” in a bit, but first, let’s take a look at the influence of temperature on the separation.
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John Dolan is considered to be one of the world’s top experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years, in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.