Proteomics workflows do not answer every question in the same way. Bottom-up approaches support broad protein identification and high-throughput analysis, while top-down strategies preserve intact proteins to reveal proteoforms, sequence variants, and post-translational modifications in greater detail. This infographic breaks down how each workflow operates, where each fits best, and why many labs benefit from understanding both.
In this infographic, readers will see how bottom-up proteomics moves from enzymatic digestion to peptide separation and tandem MS, and how top-down proteomics uses intact protein separation and high-resolution MS to map proteoforms directly. It also highlights the core tradeoffs between throughput, sensitivity, spectral complexity, and level of structural detail.
Download the infographic to get a clear, side-by-side view of these two foundational MS-based proteomics strategies and the value of combining them for a more complete picture of protein identity and function.
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