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Learn how to determine HPLC LOD and LOQ using the calibration curve method per ICH Q2(R1), including equations, assumptions, and common pitfalls.

Chromatographic Measurements, Part 5: Determining LOD and LOQ Based on the Calibration Curve

Articles

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This Edition of HPLC solutions is looking at how peaks are integrated the possible problems associated with it.

Peak Integration, Part 1: How It Is Done

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Learn about common peak integration errors in HPLC and practical solutions to improve your chromatogram accuracy.

Peak Integration Errors: Common Issues and How to Fix Them

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This Edition of HPLC solutions is about Gradient Proportioning Valve (GPV) Test, which is a test to make sure the various components are working properly.

Gradient Proportioning Valve (GPV) Test

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A collection of mass spectra of known compounds and the program used to search a spectrum of an unidentified compound are two different entities. The results obtained depend on both. Full and correct citations should be the norm - find out how to do this correctly.

Key Notes on Citing Spectral Library Searches

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This Edition of HPLC solutions is talking about the peak resolution as a measure of separation quality.

Back-to-Basics #6: Resolution

The importance of quantifying the peak tailing as part of the system suitability measurements is shown in this technical article.

Back-to-Basics #5: Tailing

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A discussion on the impact of detector acquisition rate and amplifier settings in the LOD and LOQ.

Pay Attention to Acquisition Rate and Detector Range

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This technical article considers some factors affecting the chromatographic selectivity.

Back-to-Basics: Selectivity

In a series of special video interviews done recently, Professor Pernendu (Sandy) K. Dasgupta, University of Texas in Arlington, shared his thoughts on IC including topics such as origins and possible developments of reagent-free IC, why perchlorate and ambient ion monitoring.

Conversations with world renowned IC Expert

In this first in the series of blogs about internal standards, we look at how they work in HPLC and LC-MS applications.

Internal Standards #1: How Does It Work?

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This Edition of HPLC solutions is dealing with the problems arising from mobile phase additive, specifically TFA.