Illustration of metabolites represented as bubbles

metabolite-identification

SCIEX

Separation Science

Metabolite identification is crucial in drug development, LC-MS with CID fragmentation is the gold standard for this analysis, but are there options to enhance data confidence?

Improving Confidence in LC-MS/MS Metabolite Identification

Articles

LC-MS characterization of GLP-1 agonists showing a multiply charged electrospray envelope deconvoluted to an intact mass.

lc-ms-characterization-of-glp-1-agonists

Chromatography separates; mass spectrometry identifies. How electrospray ionization charges these peptides, how to turn a charge envelope into an intact mass, and how to read fragmentation data to pinpoint oxidation, deamidation, and truncation.

LC-MS Characterization of GLP-1 Agonists: Ionization and Data Interpretation

GLP-1 related substance testing and regulatory review of synthetic peptide impurities against ICH and FDA thresholds.

glp-1-related-substance-testing

Synthetic peptides are not biologics, and that single distinction governs how their impurities are controlled.

GLP-1 Related Substance Testing: Regulatory Considerations for Synthetic Peptide Impurities

Ion-pairing chromatography of GLP-1 and charged peptides showing a separation tuned by perfluorinated acid reagents.

ion-pairing-chromatography-for-glp-1

Charged peptides resist clean separation until you give their positive charge something to hold onto. How ion-pairing works, how reagent chain length and concentration tune it, and how to keep the method rugged and mass-spectrometry-friendly.

Ion-Pairing Chromatography for GLP-1 and Charged Peptides

Reversed-phase HPLC column selection and mobile phase for GLP-1 peptide analysis.

reversed-phase-hplc-for-glp-1-peptides

Column and mobile phase are not separate choices but one coupled decision. A practical guide to selecting stationary-phase chemistry, particle format, and pore size, and the ion-pairing reagent, organic modifier, and pH that make them work

Reversed-Phase HPLC for GLP-1 Peptides: Column Selection and Mobile Phase

Scientist developing a UHPLC method for GLP-1 impurity profiling, optimizing a gradient on a core-shell column

glp-1-uhplc-method-development

Building a GLP-1 related-substance method that survives transfer is an exercise in design, not improvisation. Here is how to work from a defined target profile through column scouting, mobile-phase and gradient choices, robustness, and validation.

GLP-1 Receptor Agonist Impurity Profiling: UHPLC Method Development Guide

Analytical chemist running GLP-1 peptide analysis on a UHPLC system with a chromatogram of resolved impurity peaks.

glp-1-peptide-analysis

A technical guide to GLP-1 peptide analysis: chromatography, impurity profiling, characterization, and regulatory expectations for synthetic peptides.

GLP-1 Peptide Analysis: Chromatography Methods, Impurity Profiling, and Analytical Challenges

Abstract representation of chromatographic method validation with molecular structures.

chromatographic-method-validation-2228457760

Discover how to transition from regulatory anxiety to routine laboratory success with foundational guidelines from industry experts.

Demystifying Chromatographic Method Validation with John Dolan: Regulatory Guidelines and Lab Best Practices

Laboratory setup for high-performance liquid chromatography (HPLC)

chromatography-basics

Transition from analytical guesswork to routine chromatographic success with foundational guidelines from industry experts.

Chromatography Basics with John Dolan: Core Parameters and System Suitability

Biopharmaceutical process highlighting ultrafiltration-diafiltration with vials.

ultrafiltration-diafiltration

Real-time PAT in UF-DF shifts manufacturing from reactive testing to proactive in-process control, improving product quality and reducing the reliance on delayed offline measurements.

Real-Time PAT in UF-DF: Enhancing In-Process Control to Accelerate the Path to Real-Time Release

Abstract representation of nanofluidic UV-Vis spectroscopy

nanofluidic-uv-vis-spectroscopy

How shrinking sample volumes to the nanoscale is extending UV-Vis spectroscopy into ultra-low-volume analytical workflows

The Rise of Nanofluidic UV-Vis Spectroscopy

Electrical signals illustrating power quality issues in a lab

istock-2219985709-nxt-power-custom

NXT Power

Identify the limitations of standard surge protection and learn why isolation transformers reduce facility-generated electrical noise.