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Videos & Tutorials

Separation Science offers free learning from the experts covering videos & tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, spectroscopy, sample preparation and related analytical techniques.

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Featured Videos & Tutorials

(U)HPLC Analysis of Biopharmaceuticals: Quality Attributes and How to Analyse Them

On-demand Tutorials

Andrea Krumm (Tosoh Bioscience)

In this tutorial series you will learn the basics of (U)HPLC analysis of biotherapeutics, such as monoclonal antibodies and other proteins. We explain which critical quality attributes need to be analyzed and which (U)HPLC methods are suitable for these purposes. In addition, we provide tips and tricks from our chromatography experts for successful method development.

[LC Tutorials] Size-Exclusion Chromatography (SEC): principles and techniques to obtain reliable protein size-based-separations

Open Access Tutorial

Bill Warren (Waters Corp.)

Size-Exclusion Chromatography (SEC) is a powerful technique for protein characterization that requires an understanding of the principles to be successful. In these Learning Modules, we will discuss the history and science of SEC, its strengths and limitations, and how to get the most from this important analytical technique. We’ll also discuss the analytical challenges with a focus on monoclonal antibody separations and the effective use of LC-based, size-exclusion chromatography to obtain reliable mAb aggregate, monomer, and fragment analyses.

[LC Tutorials] How to Improve Method Robustness through Control of Unwanted Interactions

Open Access Tutorial

Kim Haynes (Waters Corp.)

This course is designed to take you from beginner to advanced in controlling the interactions within your chromatographic separations. It will cover how to improve method robustness through the prevention of analyte loss, with improved confidence in the quality of your data.

[LC Tutorials] Understanding LC Column Attributes - What's Important for Your Chromatography?

Open Access Tutorial

Jonathan Turner (Waters Corp.)

This course is designed to take you from beginner to advanced in understanding column selection and method development. It will cover the key aspects of the LC column from the base particle to the bonded phase, and even the hardware.

Troubleshooting & Tips for Atomic Absorption

On-demand Tutorial

Eric Vanclay (Agilent, Australia)

In this series of tutorials you will learn about types and performance of AA lamps, improving performance, maintenance, consumables and resources. Increase your knowledge of and skills in atomic absorption spectroscopy by viewing these educational presentations.

Expert Series: Ion Chromatography Fundamentals

On-demand Tutorial

Dr Joachim Weiss, Ph.D (Thermo Fisher Scientific)

We kick off the Expert Series with tutorials Ion Chromatography fundamentals by Technology Expert, Dr Joachim Weiss, Ph.D, on a comprehensive introduction of IC, comparisons of competing methods, basics of anion-exchange and cation-exchange, and various detection methods.

GPC / SEC Good Practice & Troubleshooting Tutorials

On-demand Tutorial

Dr Daniela Held (PSS)

In this learning series, you'll be provided with video tutorials discussing the best practice and troubleshooting solutions for gel permeation chromatography / size exclusion chromatography: 1) What Should You Know Before Injecting Your First Sample, 2) Pressure Problems, 3) Unexpected Peak Shapes and 4) Choosing the Column.

Conversations with world renowned IC Expert

Interview Series

Professor Pernendu (Sandy) K. Dasgupta, University of Texas in Arlington

In a series of special video interviews done recently, Professor Pernendu (Sandy) K. Dasgupta, University of Texas in Arlington, shared his thoughts on IC including topics such as origins and possible developments of reagent-free IC, why perchlorate and ambient ion monitoring.

HPLC, LC-MS, GC and GC-MS training

Self-paced online course with validated learning and certificate of completion

Analytical Training Solutions | Separation Science

HPLC, LC-MS, GC and GC-MS training courses on Analytical Training Solutions have been divided into a number of modules, with each module focusing on one particular aspect of the overall course. The modules have been created in a logical manner, and by starting with the first module and working through them in the order provided creates the best learning environment for the user. In addition, as each course is divided into modules it is then very simple to find a particular section again in the future; for example, if you want a recap on a particular subject.

Featured Content Hubs

Separation Science Food Safety Solutions Hub

Content Hub


Separation Science, in collaboration with LECO Europe, offers this free, learning hub covering methods and applications focusing on technologies to enhance your food analyses and increase data quality and sample throughput. Here you'll find webinars, an online workshop, application notes, an eBook and other educational resources to cover all of your food analysis needs.

Latest Videos & Tutorials

Food & Beverage Analysis

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Pharmaceutical Analysis

Bio Clinical Analysis

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Gas Chromatography

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