To develop a more in-depth understanding of gas chromatography-mass spectrometry. The framework for GC-MS quantitation is explained. Topics and examples include integration parameters, linear and non-linear calibrations, calibration table choices, making prudent choices for quantification and qualification ions, external and internal standard reports and calibration table entries.
You should take this course if you are a user of gas chromatographs who is looking to further develop their method development and troubleshooting skills in GC-MS. A key demographic are companies that provide their employees with the yearly skill set development objectives. Even though supported by management, fulfilment of these objectives is difficult to accomplish through traditional training due to scheduling issues and frequent work-related interruptions. The online tutorial approach provides a unique solution by being accessible from anywhere with internet access, and at any time.
This course covers:
The framework for GC-MS quantitation is explained. Topics and examples include integration parameters, linear and non-linear calibrations, calibration table choices, making prudent choices for quantification and qualification ions, external and internal standard reports and calibration table entries.
- General Concepts and Terminology
- Setting up a Calibration Table for Quantification
- Picking Primary (“Quant”) and Secondary (“Qual”) Ions
The relevant aspects of tuning a quadrupole MS for GC data acquisition are covered. An explanation of different types of tunes is provided. Example parameters and setpoints are explained as well as their effects on results. Development and use of custom tunes to meet method-specific requirements are presented as are concepts of dynamic ramping of source parameters. How to use historical tune results to track system performance changes is also explained.
This module has a complementary quiz. By correctly answering the quiz questions for all of the modules as part of the course you will be able to download your certificate of completion. Types of questions to expect are:
- What does quantification in mass spectrometry usually based on?
- What influences peak height?
- Can one generate an accurate and precise quantitative result with a non-linear calibration curve?
- What is the simplest form of calibration?
- What is the effect in the results when there is a matrix effect?
- What is typically the primary (“quant”) ion?
- What is the function of qualifier ions?
- How can the choice of primary ion be improved?
- How should calibration data be acquired?
- Why does an MS calibration more complicated than with single dimension GC detectors?
- What is the best ion selection for SIM quantification?
- Why does selected ion monitoring preferred for an analysis?
- What is the biggest difference between extracted ion and selected ion (SIM) quantification?
- What should one do when setting up a SIM table?
- What can we get by specifying SIM ions to the highest mass precision (e.g., to the nearest 0.1 u)?
Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique education resource for analytical chemists. Currently, we offer HPLC training, LC-MS training, GC training and GC-MS training.