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Welcome to Separation Science

 
 

Separation Science is the leading global portal for fundamentals, best practice and troubleshooting information for users of chromatographic and mass spectrometric techniques. Featuring articles, applications, webinars, workshops and conferences, Separation Science is the industry's 'go to' information resource.

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Webinars

Upcoming

Optimization of UHPLC Methods for Food and Beverage Analysis

Presenters:  Aaron Lamb & Dr Susanne Fabel (Thermo Fisher Scientific)
Sponsor:  Thermo Fisher Scientific
Date: Tuesday, 28 March, 2017
Times: 8 am PDT / 11 am EDT / 4 pm BST / 4 pm CEST

 

On-Demand

SIFT-MS: Rapid, Selective Trace Gas and Headspace Analysis

Presenters:  Mark Perkins (Anatune Limited, Cambridge, UK) and Vaughan Langford (Syft Technologies, Christchurch, New Zealand)
Sponsor:  Syft Technologies

 

Routine Quantitative Pesticide Analysis in Various Food Matrices using a GC/Q-TOF

Presenter:  Dr Klaus Wilmers (CVUA-MEL, Germany)
Sponsor:  Agilent Technologies

 

Best Practices in Karl Fischer Titration

Presenters:  Bettina Straub-Jubb (Merck KGaA, Darmstadt, Germany) & Valeria Gärtner (Mettler Toledo GmbH, Schwerzenbach, Switzerland)
Sponsors:  Merck KGaA, Darmstadt, Germany and Mettler Toledo GmbH

 

UV-vis Spectrophotometric Analysis of Water, Food, and Beverages

Presenters:  Carlin Klein, Rick Blenkemeier & Abebe Belay Gemta
Sponsor:  Merck KGaA

 

Make Productivity Happen – Optimizing your GC System

Presenter:  Vanessa Abercrombie (Agilent Technologies, USA)
Sponsor:  Agilent Technologies

 

Optimizing the Extraction of Pesticide Residues in Edible Oils

Presenter:  Eugene Chang (United States Food and Drug Administration)
Sponsor:  Agilent Technologies

 

Better, Faster Solutions for Biopharmaceutical Characterization

Presenters:  Ning Tang (Application Engineer, Agilent Technologies) and Jordy Hsiao (Application Engineer, Agilent Technologies)
Sponsor:  Agilent Technologies

 

SPME Method Development for Food Analysis

Presenter:  Érica A. Souza-Silva (Federal University of Rio Grande do Sul, Brazil)
Sponsor:  MilliporeSigma

 

Developing, Testing and Troubleshooting Chromatography Method Transfer

Presenter:  Tom Wheat (Waters Corp., USA)
Sponsor:  Waters

 

The Use of Thermal Desorption for the Analysis of Electronic Cigarette Aerosols

Presenters:  Justin Frosina (British American Tobacco, UK) & Caroline Widdowson (Markes International, UK)
Sponsor:  Markes International

 

eSeminars

Advances in Forensics & Toxicology 2016 eSeminar

Separation Science, in collaboration with its sponsors Gerstel, Bruker and Shimadzu, has developed an on-demand version of its third online eSeminar focusing on advances in forensics and toxicology. This online educational event will provide forensic scientists, toxicologists and those who work in laboratories within the forensic and toxicology industries with updates and guidance on the latest methods, protocols, processes and applications of chromatography, mass spectrometry and related techniques with key experts providing practical insights into their respective fields.

Speakers include: Christophe Stove, Laura Tretzel, Andreas Thomas, Oliver Lerch, Tobias Kieliba, Bjoern Moosmann, Angelika Köpf, Uwe Aulwurm and George Hime 

Advances in Food Safety Analysis eSeminar – Contaminants, Toxins & Allergens

Separation Science, in collaboration with sponsors Bruker, Markes International, PerkinElmer and SCIEX, has developed an on-demand eSeminar focusing on new methods and applications in food safety analysis – particularly for the analysis of contaminants, toxins and allergens. This unique on-demand event brings together some of the leading exponents in food safety analysis and provides an interactive learning environment for scientists working in these fields.

Speakers include: Michael Walker, John E. Jones III, Wilhad M. Reuter, Daniel Beach, Lucie Humbert, Natasha Spadafora, Laura McGregor and Kathy Ridgway

3rd Advances in Environmental Analysis eSeminar

Separation Science, in collaboration with its sponsors Agilent Technologies, Markes International and PerkinElmer, has developed its third online eSeminar focusing on advances in environmental analysis. This unique on-demand event brings together some of the leading exponents in the field of environmental analysis and will provide an interactive learning environment for scientists working in this industry.

Speakers include: Jonathan J. Grandy, Craig Marvin, John Entwisle, Dmitriy Malinovskiy, Lee Marotta, Jamie Brown, Carol Meyer, Roberta Provosta and Nicola Watson

2nd Advances in Forensics & Toxicology eSeminar

Separation Science, in collaboration with its sponsor Gerstel, has developed an on-demand online eSeminar focusing on advances in forensics & toxicology. This unique event brings together some of the leading exponents in the forensics and toxicology industries and provides an interactive learning environment for scientists working in these fields.

Speakers include:  Adam C. Lanzarotta, Oliver Lerch, Alberto Salomone, Hooi Yan Moy, Sarah Wille and Fabio Vaiano

UV-vis Spectrophotometric Analysis of Water, Food, and Beverages

Separation Science, in collaboration with Merck KGaA, has developed an online eSeminar focusing on the use and application of UV-vis spectrophotometry for the analysis of water, food, and beverages. This unique event provides scientists with updates on the latest issues faced by individuals working in these challenging analytical fields. It brings together a selection of technology experts and provides an interactive learning environment and online resource.

Speakers include:  Carolin Klein, Rick Blankemeier and Abebe Belay Gemta

On-Demand

Routine Quantitative Pesticide Analysis in Various Food Matrices using a GC/Q-TOF

Presenter:  Dr Klaus Wilmers (CVUA-MEL, Germany)
Sponsor:  Agilent Technologies

In this webinar you will discover an effective workflow strategy using a GC/Q-TOF for routine pesticide screening applications.

Make Productivity Happen – Optimizing your GC System

Presenter:  Vanessa Abercrombie (Agilent Technologies, USA)
Sponsor:  Agilent Technologies

In this webinar you will be provided with tips for finding out if you are using the right parts for the right job, how to troubleshoot this and how to easily transfer your methods from standard columns to high efficiency columns when you need to reduce analysis time.

SPME Method Development for Food Analysis

Presenter:  Érica A. Souza-Silva (Federal University of Rio Grande do Sul, Brazil)
Sponsor:  MilliporeSigma

This webinar focuses on the implementation of SPME methods for food analysis, including optimization of the factors affecting SPME efficiency; special considerations for targeted and non-targeted applications; quantitation in SPME; and novel SPME devices and future directions.

Optimizing the Extraction of Pesticide Residues in Edible Oils

Presenter:  Eugene Chang (United States Food and Drug Administration)
Sponsor:  Agilent Technologies

To meet or exceed the requirements of recovery and sensitivity of pesticide residues in edible oil analysis, a key factor is to keep the lipids out from the sample extract without significantly losing the residues. We evaluated the clean-up efficiency of new "Enhanced Matrix Removal-Lipid" sorbent by monitoring phosphor lipids and then developed an optimized extraction method for high fat food.

Better, Faster Agilent Solutions for Biopharmaceutical Characterization

Presenters:  Jordy Hsiao & Ning Tang (Agilent Technologies, USA)
Sponsor:  Agilent Technologies

We illustrate that HCPs can be confidently identified using a MS1 profiling and iterative directed MS strategy to serve as a superior acquisition method relative to the traditional shotgun MS/MS approach. Moreover, the beneficial use of different fractionation strategies as a first dimension for HCP analysis using 2D-LC-MS will be presented.

Direct Analysis of Food and Beverages using SPME-GC-MS/MS - No Sample Cleanup, Automated and Highly Specific

Presenters:  Elena Ciceri (Thermo Fisher Scientific, Italy)
Sponsor:  Thermo Fisher Scientific

In this informative webinar, we will explain how to optimize and validate a simple, inexpensive, and rapid method to confirm and identify biologically active flavorings using automated headspace solid-phase microextraction coupled with GC-MS/MS.

Self-Paced Training

AVAILABLE COURSES

HPLC Basics, Equipment, and Troubleshooting

What does it cover?

“HPLC Basics, Equipment, and Troubleshooting” explains chromatography in practical terms from the ground up.
Here’s what you’ll learn in this online course:
• The basic principles of HPLC
• The five fundamental HPLC modes
• The easy and logical way to adjust experimental variables to get a good separation
• The best approach to use for various chromatographic applications
• How to maximize column life
• Special techniques such as gradient elution, quantitation, and sample pretreatment
• The operating principles of each module in an HPLC system
• Proven techniques for systematic problem-solving and instrument maintenance
• The most effective, timesaving, money saving approaches to preventing common hardware problems and method failures.

What will I get from this is course?

You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient. What you learn will demystify your instrument. You’ll find that all of the perplexing and frustrating problems your experience have simple and logical solutions. And, better yet, you’ll learn that most of these problems are preventable. The techniques you learn from this course will make you more effective at work and save you big headaches! If you’ve never taken a formal course or if you need a refresher, it’s time to learn HPLC the right way. Students tell us that this “extremely practical course” is a must for everyone who uses HPLC.

Advanced HPLC Method Development Using Quality by Design

What does it cover?


The central tenant of Quality by Design (ICH Q8) is that quality cannot be tested into a product – instead it must be designed into the product. When the product is an HPLC method, QbD strategies can guide the development process to result in a standardized method development prcedure, more easily validated methods, and methods that are easier to use and adjust in routine applications.
 “Advanced HPLC Method Development Using Quality by Design” is an intensive online class that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods.

Introduction to Gas Chromatography

What does it cover?

The ‘Introduction to Gas Chromatography’ On-Line Tutorial is basic introduction to the theory and practice of gas chromatographic analysis. It is designed to bring GC users to a more comfortable and capable level of proficiency in instrument operation, optimization, and troubleshooting. Lessons are presented in a visually engaging and accessible manner through narrated on-line video presentations. In full recognition of the difficulty in setting large blocks of uninterrupted time for training; the lessons are presented in short segments that can be easily fit into even the busiest schedules.
• This course presents the basic processes and instrumentation involved with GC analysis.
• The level of material is specifically designed for GC users without extensive formal training.
• Applicable theory is covered to the extent that it helps guide best practices, method optimization and effective troubleshooting.

What will I get from this course?

Upon successful completion of the tutorial, trainees will have increased confidence and skills in using gas chromatographs. They will better understand how GC works, be more competent at detecting and diagnosing problems, and will be better at interpreting the quality of analytical results. In the end, trainees will be more capable and comfortable using gas chromatographs.

Intermediate Gas Chromatography

What does it cover?

The Intermediate Gas Chromatography tutorial is designed for GC users who have a fundamental understanding of how gas chromatography works and who are ready to develop a more in-depth understanding of the technique. As with the ‘Introduction to Gas Chromatography’ course, the ‘Intermediate Gas Chromatography’ course is organized into sections and modules. The sections focus on a given theme and the modular structure underneath exposes students to the information within time-effective sections. The ‘Intermediate Gas Chromatography’ course is a logical next step after completing the ‘Introduction to Gas Chromatography’ course.

What will I get from this is course?

Upon successful completion of the course, trainees will have increased knowledge and skills in development of gas chromatography methods. They will gain an understanding of both qualitative and quantitative methods, as well as expanding their skills to encompass areas such as fast GC, and further developing maintenance and troubleshooting abilities.

LC-MS/MS for Chromatographers

What does it cover?

With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector. You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

What will I get from this course? 

You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise. You will receive a printed copy of the approximately 515 slides included in this course to supplement your note-taking as you view the class. 

Articles

HPLC Solutions #135: Internal Standards: Part 2 - What Makes a Good Internal Standard?

In the previous instalment of HPLC Solutions (HPLC Solutions #134) we took a brief look at why we might decide to use an internal standard and how a calibration plot is constructed and used in HPLC analysis. This time, we’ll consider some properties that are necessary when choosing a compound to use as an internal standard. These are listed in the figure.

 

It’s All Made of Plastic!

‘Plastic’ has become something of a dirty word. It makes us think of trash and environmental pollution, inferior quality, mass-produced goods, and excess. But if you take a look around, you will realize that a lot of the objects that surround us – including those that we couldn’t live without – are also made of plastic.

 
 

Product Showcase

Wyatt Announces Its High-Throughput Dynamic Light Scattering Instrument with 21CFR11 Compliance

Wyatt Technology announces the release of its 21CFR11-compliant software for its groundbreaking DynaPro® Plate Reader II (PRII). The DynaPro PRII is said to be the only high-throughput, automated dynamic light-scattering instrument capable of measuring the size and stability of biopharmaceuticals and nanoparticles directly in microtiter plates, including the most popular 96-, 384- and 1536-wellplate versions.

 

Shimadzu's GCMS-TQ8050 Triple Quadrupole GC-MS

Shimadzu has released the GCMS-TQ8050 Triple Quadrupole GC-MS offering both robustness and ultra-high sensitivity. In addition to detecting ultra-trace concentrations of components down to femtogram level, it can also maintain those ultra-high sensitivity levels for long periods. This makes it an ideal GC-MS/MS system for applications requiring ultra-trace analysis, such as analysing ultra-trace concentrations of persistent organic pollutants (POPs) or endocrine-disrupting chemicals in the environment or analysing impurities in pharmaceuticals.

 

Peak Scientific introduces bench-top nitrogen gas generator system

Peak Scientific has announced the launch of its latest bench-top nitrogen gas generator system, called Solaris. Developed to offer a compact and convenient gas source for labs using ELSD (Evaporative Light Scattering Detector) instruments and Compact Mass Spectrometers, Solaris provides a consistent source of nitrogen gas for these applications, typically requiring lower flow-rates and specific purity. 

 

Peak Scientific introduces innovative gas subscription service

Peak Scientific, a global leader in on-demand gas solutions for laboratories, has recently announced the launch of FLEXFLOW, a new monthly gas subscription service. Available initially for customers in North America and some territories in Europe, the unique service gives customers a new alternative to high pressure cylinders, without the need to purchase and maintain generator equipment. FLEXFLOW is designed for customers with LC-MS instruments in their laboratory who are looking for a more convenient and cost-effective solution for instrument gas.

 

Tosoh Bioscience Introduces TSKgel® Protein A-5PW HPLC Columns

Tosoh Bioscience LLC, a provider of chromatographic solutions for the separation of biomolecules, has announced the introduction of the TSKgel Protein A-5PW column. This column is specifically designed for the rapid separation and robust monoclonal antibody titer quantification during cell line selection or upstream bioprocess optimization and control. The TSKgel Protein A-5PW column is now available for sale in the Americas from Tosoh Bioscience LLC.

 

Markes launches new ‘xr’ series of thermal desorption instruments

Markes International (Llantrisant, UK) has announced the launch of a new family of thermal desorption instruments for GC–MS at the Analytica 2016 tradeshow in Munich, Germany.
The new products, which form the ‘xr’ series, pre-concentrate organic vapours sampled using sorbent tubes, canisters and on-line air streams, and as well as options for automation, incorporate powerful new advances that extend the capabilities of analytical laboratories analysing volatile and semi-volatile organic compounds (VOCs and SVOCs) in air and materials.

 

Application Handbook: Clinical

With almost 140 pages, the new Shimadzu Application Handbook “Clinical” combines 47 real life applications and most advanced technologies and solutions for clinical research. All major analytical instrumentation methods are covered, such as chromatography, mass spectrometry, spectroscopy and life science lab instruments. The book is free of charge and can be downloaded (17 MB) at: www.shimadzu.eu/clinical

 

Intelligent Solutions for UHPLC / HPLC Method Development – ACE Method Development Kits

ACE Method Development Kits are designed to maximise selectivity – the key to resolution in chromatography. A complete range of kits for all requirements is available, including kits featuring porous, solid-core and bioanalytical 300Å particle columns. These kits group together the essential column chemistries for method development.

 

Markes International launches analytical chemistry awareness campaign

Markes International (Llantrisant, UK), in association with Exterion Media (UK) Ltd, is launching a major digital poster campaign to highlight the value of the analytical chemistry industry to society.

 

Videos demonstrating GC–TOF MS instrument control and data-processing software

Markes International (Llantrisant) has announced the release of a set of six videos explaining the key features of its TOF-DS™ software package.

 

Polymer-based SPE columns

Biotage announces the addition of new polymer - based SPE columns to their EVOLUTE ® EXPRESS range. EVOLUTE® EXPRESS is a novel family of plates, and now columns, that simplify polymer-based SPE by employing a streamlined three step protocol; Load-Wash-Elute. By using this approach and removing the conditioning and equilibration steps, necessary with traditional bio-analytical SPE procedures, processing and method development times are dramatically reduced without loss of analyte recovery or method robustness.

 

MIURA GO-xHT - A powerful clean-up system for dioxins and PCBs

Shimadzu is the official distribu­tor of the revolutionary GO-xHT clean-up system from MIURA Institute of Environmental Science. The GO-xHT targets dioxins and PCBs (polychlorinated biphenyl) analyses of food and feedstuff. The combination with Shimadzu’s GCMS-TQ8040 triple quadrupole fulfills the new EU 589/2014 regulation on analysing dioxins and PCBs samples.

 
 

Latest HPLC Solutions

HPLC Solutions #135: Internal Standards: Part 2 - What Makes a Good Internal Standard?

In the previous instalment of HPLC Solutions (HPLC Solutions #134) we took a brief look at why we might decide to use an internal standard and how a calibration plot is constructed and used in HPLC analysis. This time, we’ll consider some properties that are necessary when choosing a compound to use as an internal standard. These are listed in the figure.

 
 
 

This series of three presentations, produced in collaboration with Phenomenex, will offer practical advice and guidance on the use of HPLC. The series comprises:

Part I: Understanding HPLC Particle Technology
by Jeff Layne (Manager, Product Management and Technical, Phenomenex, USA)
AVAILABLE NOW

Part II: A Survey of Reversed Phase-HPLC Stationary Phases – from Selectivity to Applicability
by Jeff Layne (Manager, Product Management and Technical, Phenomenex, USA)
AVAILABLE NOW

Part III: Basic HPLC Troubleshooting and Column Care
by Scott Krepich (Technical Manager, Phenomenex, USA)
AVAILABLE NOW

This on-demand webinar covers selected ion flow tube mass spectrometry (SIFT-MS), a technology that provides a significant advance in trace gas and headspace analysis. The presentation examines how application of SIFT-MS can deliver competitive advantage in the contract laboratory because of high sample throughput and its ability to analyse chromatographically challenging species. It also demonstrates how direct gas analysis opens new research and on-line monitoring opportunities.

High-Throughput Dynamic Light Scattering Instrument with 21CFR11 Compliance

Wyatt Technology announces the release of its 21CFR11-compliant software for its groundbreaking DynaPro® Plate Reader II (PRII). The DynaPro PRII is said to be the only high-throughput, automated dynamic light-scattering instrument capable of measuring the size and stability of biopharmaceuticals and nanoparticles directly in microtiter plates, including the most popular 96-, 384- and 1536-wellplate versions.

Click for more information >

This on-demand event includes two presentations:

Sensitive and Reliable Measurement of ortho-Phosphate in Ground and Surface Water
by Carolin Klein

Importance of Phosphonates Analysis in Boiler and Cooling Water and Measurement Techniques in Spectroquant
by Onur Akbulut

View the on-demand presentations including:

Automated Malt Analysis using Discrete Analyzers
by Aaron McLeod

Direct Analysis of Food and Beverages using SPME-GC-MS/MS - No Sample Cleanup, Automated and Highly Specific
by Elena Ciceri

HPAE-PAD for Prebiotic Ingredient Characterizations
by Parul Angrish & Manali Aggrawal

Applying ICP-MS to Speciation and Quantitative Analysis of Arsenic in Foodstuffs and Beverages
by Antonio Signes-Pastore & Simon Nelms