Home / Blog

Separation Science

Separation Science (www.sepscience.com) offers free learning from the experts covering methods, applications, eBooks, webinars, eSeminars, expert forums, videos, tutorials and training for users of liquid chromatography, gas chromatography, mass spectrometry, spectroscopy, sample preparation and related analytical techniques.

Stay ahead and improve your skills! Receive tailored information on new separation science methods and applications.Register for free learning now >>

Featured Educational Resources

The Power of Data Analytics: Driving Laboratory Efficiency and Discovering New Insights

28 June, 2022

David Hardy (Manager of Data Analytics and Visualization, Thermo Fisher Scientific)

In this webinar, join Dr. David Hardy, from Thermo Fisher Scientific, as he reveals the benefits of data analytics and visualization tools, and explains how laboratories can effectively use these to optimize their data to drive efficiency and innovation.

Valisure’s Identification of Benzene as a Pervasive Contaminant in the Consumer Products Industry

21 & 22 July, 2022

Kaury Kucera and David Light (Valisure)

This presentation describes the identification of benzene in a variety of consumer products, such as hand sanitizers, sunscreens and body sprays and how Valisure provides independent quality assurance throughout the supply chain.

New Methodology for the Analysis of Pesticides in Fruits and Vegetables by GC-HRMS


Ramkumar Dhandapani (Phenomenex) and Javier Arrebola (University of Almería)

Discover some invaluable method optimization tips and tricks for pesticide analysis of real sample matrices, including fruits and vegetables, by gas chromatography/high-resolution mass spectrometry (GC-HRMS). Learn how the use of HRMS is increasingly being incorporated to routine work-flow laboratories because of its power to discriminate analytical information and capability to avoid matrix interferences and how it also helps to evaluate the performance of the tested GC column.

Global PFAS Testing Virtual Symposium: Advances in Occurrence & Testing


Frederik Zietzschmann (Laboratory of Berliner Wasserbetriebe), Stephen Somerville (Pace Analytical), Dr. Nobuyoshi Yamashita (AIST), Dr. Bradley Clarke (University of Melbourne), Dr. Arjun Venkatesan (Stony Brook University), Dr. Erin Baker (North Carolina State University)

Learn about the latest advances in quantification of legacy and novel PFAS; developing and accreditation for routine and regulatory PFAS methods including EPA and EU guidelines; novel analytical techniques to identify new PFAS; and testing methods optimized for water, air, commercial products, textiles, soil and others.

Introducing Agilent’s CrossLab CS Electronic Leak Detector and ADM Flow Meter: Two GC Diagnostic Tools, One Convenient Package


Paul Tripp and Angela Smith-Henry (Agilent)

Leaks are problematic for any operation that uses gas tubing and fittings. Even small gas leaks can lead to safety issues, reduced productivity, and increased costs. Here the presenters introduce the CrossLab CS Electronic Leak Detector and ADM Flow Meter — two GC diagnostic tools in one cost-effective, convenient package.

Powering Up Battery Materials Analysis using ICP-OES and ICP-MS


Simon Nelms and Sukanya Sengupta (Thermo Fisher Scientific)

This presentation provides a complete overview of how ICP-OES and ICP-MS analysis supports every step in the battery manufacturing value chain, from raw material screening and refining, through battery cell material research and QA/AC, to recycling and environmental analysis. The basics of ICP-OES and ICP-MS technology are explained and selected analysis examples demonstrated.

Practical Challenges in PFAS Extraction for Water Samples and How to Address Them


Bill Jones (Director of Chemical R&D, Biotage LLC)

This practical, experience-based webinar is useful for anyone involved in or performing method development for PFAS in aqueous matrices using a range of methods. You will gain more information on the pervasiveness of PFAS compounds in a typical extraction laboratory and the steps that can and should be undertaken to combat them.

Optimized Workflow of POPs Analysis in Environmental and Food Matrices Using Semi-Automated Cleanup and GC-MS/MS


Ruud Addink (Technical Director, Fluid Management Systems)

This presentation discusses several clean-up methods with data and shows how the Agilent 7010 GC-MS/MS instrument can be used for state-of-art dioxin and PCB work at levels of sensitivity equal to or better than magnetic sector instruments.

Electron Activated Dissociation (EAD) MS/MS for the Structural Elucidation of Lipids


Sophia Laposchan (Technical University of Munich, Germany)

By viewing this presentation you will gain a basic understanding of using electron activated dissociation in MS/MS analysis for structural elucidation of lipids; and learn how EAD can distinguish structural isomers of various lipid classes and how this powerful technique adds value over other fragmentation techniques.

Knowing How to Make ICP-MS Easier


Gareth Pearson (Agilent, Spectroscopy Technology Innovation Centre, Melbourne, Australia), Glenn Woods (Agilent, Centre of Excellence, Manchester, UK), and James Dellis (Agilent, Spectroscopy Technology Innovation Centre, Melbourne, Australia)

Learn how Easy-fit supplies can simplify your workflow, discover tools that can help you get your ICP-MS configured right for your analysis, take a look at ICP-MS MassHunter software, and learn about some of the simple software tools and time-saving tips that can help you get the very best out of your ICP-MS.

Gas Chromatographic Analysis of Biofuels – A Survey of Solutions to Assure Quality and Performance for Today’s Energy Needs


Scott Hoy (Application Chemist, Agilent) and Jim McCurry (Senior Application Chemist, Agilent)

Several gas chromatographic (GC) methods have been developed to help producers and users of biofuels meet these specifications. This seminar presents solutions built on Agilent’s Smart Gas Chromatographic instruments. Analysis of several classes of biofuel samples demonstrates the performance of these solutions in meeting the requirement of industry standard methods.

PAMS Analysis during COVID-19 Lockdowns in New York City


Pete Furdyna (Applied Analytical Chemist, NYSDEC, USA) and Laura Miles (Senior Application Chemist, Markes International, UK)

In this presentation you will learn how NYSDEC achieved excellent data stability over extended sampling periods, how water can be effectively managed for sample integrity and to achieve sub-ppb levels of detection for ozone precursor VOCs, and the benefits of using hydrogen carrier gas for continuous, robust and unattended monitoring in remote locations.

Determination of Anions and Cations in Power Plant Water using Ion Chromatography


Peter O’Brien (EDF Energy, UK)

Learn how ion chromatography with suppressed conductivity detection is the safe and reliable method of choice to determine both µg/L (ppb) and mg/L (ppm) levels of anions and cations including transition metals in power plant waters.

Dissolution Today, Tomorrow & Beyond

On-demand webinar

Vivian Gray, Lee Ashworth, Samir Haddouchi, Andre Hermans & Bryan Crist

This expert presentation and discussion forum focuses on aspects of dissolution from development through to a marketed product. Our expert panellists discuss the role of dissolution as a quality assurance tool for product development, approval and batch release, the use of dissolution testing of novel drug formulations and how it is being used to probe dosage form behaviour.

Ask the Experts: Which Trace Elemental Instruments are Best for your Analysis?

On-demand webinar

Daniel Kutscher

This is the second on-demand instalment of the 'Instrument Selection Discussion Series' where laboratory professionals gain knowledge on the choices of instrument available and which is most recommended for specific applications.

Moving Towards Sustainable Gas Chromatography Laboratories

On-demand webinar

Ed Connor (PEAK Scientific) and Jessica Berger (Markes International)

By watching this presentation you’ll learn about the benefits of using hydrogen as a carrier gas; configuring thermal desorption–gas chromatography–mass spectrometry analytical instrumentation to work safely with hydrogen as a carrier gas as well as with helium and nitrogen; the pros and cons of different hydrogen sources; and common pitfalls when switching carrier gas and how to avoid them.

Turn your Triple Quad up to 11!

On-demand webinar

April Quinn-Paquet (Technical Product Manager, SCIEX)

You will learn about the latest features for acquisition in SCIEX OS software including automatic data processing and decision rules, which can automatically perform actions on the queue according to user-specified criteria.

Discover a Microelution Extraction Technique for Drugs of Abuse with Streamlined Sample Clean-up

On-demand webinar

Ritesh Pandya (Forensic Technical Specialist, UCT)

This presentation outlines methods for extracting a large drugs of abuse panel from urine and plasma using reverse-phase and mixed-mode sorbent chemistries in a microelution format. HPLC separation was carried out using UCT’s Selectra® PFPP column prior to the analysis on LC‐MS/MS.

Robust, Reproducible, and Quantitative Analysis of Thousands of Proteomes by Micro-flow LC-MS/MS

On-demand webinar

Bernhard Küster

This webinar offers a deep understanding of the advantages and place of microLC hyphenation with high-resolution accurate mass (HRAM) MS in proteomics research. Examples covering real biological samples analysis from cell lysates to urine, plasma, and cerebrospinal fluid, are presented.

Facilitating Data Integrity & Compliance

On-demand webinar series

This expert presentation and discussion forum focuses on data integrity and compliance, particularly in the context of QA/QC, development, and manufacturing. Experts highlight why technology companies need to put the customer at the heart of innovation, to ensure the development of solutions not just products.

How to Increase The Cost Effectiveness for HPLC Separations of Challenging and Matrix-rich Samples

On-demand webinar

Petra Lewits (Merck KgaA Darmstadt, Germany)

In this webinar the potential for cost and time savings by increasing lab productivity with faster separations and column materials providing very high matrix tolerance is discussed.

The Importance of Data Integrity in Achieving Pharma 4.0

On-demand webinar

Nicola Gardner (Thermo Fisher Scientific)

In this presentation we explore why organizations cannot continue to advance with only technology and must also understand the evolution of data integrity.

Precise Testing of Pesticides in Food using the SCIEX 7500 System

On-demand webinar

Jianru Stahl-Zeng (Global Technical Leader for Food and Environmental, SCIEX)

This presentation gives an overview of the new SCIEX 7500 System that is integrated with innovative technologies. The presenter showcases the capabilities and potential of the SCIEX 7500 System for precise pesticide testing in food.

Improve your Method Optimization for SPME

On-demand webinar

Emanuela Gionfriddo (The University of Toledo, USA)

This presentation provides an essential guide for users of SPME. It shows you ways to improve your method optimization of SPME. Fundamental and practical aspects of SPME are discussed, together with tips and tricks for SPME method development.

Continue to Improve Your Titration Process with New Tips and Tricks

On-demand webinar

Bettina Straub-Jubb (Merck KGaA, Darmstadt, Germany)

This webinar provides further tips and tricks for optimizing the titration process with a special focus on food and beverage applications.

Food Contact Materials: Role of Food & Beverage Manufacturers in Ensuring Food Safety Compliance

On-demand webinar

Dr Dnyanesh Darshane (FOBE Solutions LLC) and Dr Matthias Nold (Merck KGaA)

This webinar provides valuable insights into the risks of food contact materials and how to ensure food safety compliance.

How to Improve Your Titration Results – Optimizing Performance and Troubleshooting

On-demand webinar

Dr. Christian Haider (Head of Competence Center Titration, Metrohm AG)

In this presentation, we focus on how to get the basics of titration right so you can always trust your results including ow to select the right hardware for your titration; how to handle your hardware properly; how to choose the correct settings and parameters on your autotitrator; how endpoints are evaluated correctly and how to optimize endpoint evaluation; and troubleshooting - what to do in case you get incorrect results.

Handheld Raman Solutions for Raw Material ID

On-demand webinar

Richard Crocombe, Bülent Üstün, Ali Saleemi, Pierre-Yves Sacre and Mike Claybourn

Learn about the transformative power of handheld instruments in terms of instant actionable data and associated return on investment; key benefits of spatially offset Raman spectroscopy (SORS) for raw material ID; advantages of moving from laboratory-based testing to testing using handheld devices in the warehouse; the relative merits of handheld Raman and NIR; and challenges in method development and building models.

Making Our World Safer Through Science: Identifying Novel Biomarkers in Breath

On-demand webinar

Matthew Edwards (SepSolve Analytical) & Dr Amalia Berna (Children’s Hospital of Philadelphia)

This webinar introduces a powerful end-to-end solution for comprehensive profiling of breath volatiles, using the combination of thermal desorption, two-dimensional GC and mass spectrometry.

Ask the Agilent Experts: GC and GC/MS Scientists Answer Your Most Frequently Asked Questions

On-demand webinar

Angie Smith-Henry and Vanessa Abercrombie (Agilent, USA)

The presenters discuss some of the most frequently asked questions about GC and GC/MS. You will also gain more understanding about why different consumables are used for different applications.

Advance with Transmission Raman

On-demand webinar

Dr Julien Villaumie, Prof. Martin Warman, Dr Gary McGeorge and Mark Godfrey

Learn about the benefits for targeted implementations of TRS in pharmaceutical development and manufacture, the route to implementation of TRS and the regulatory submission for manufactured products, the important role of HPLC for successful quantitative TRS method development and verification, and the barriers and challenges including cultural, regulatory, and scientific.

Quantitation of Selected PFAS in Human Whole-Blood Utilizing VAMS (Volumetric Absorptive Micro Sampling) and Latest-Generation Triple Quadrupole Instrumentation

On-demand webinar

Andrew Patterson (Eurofins Environment Testing America - Specialty Services Division)

This webinar provides an effective approach for studying PFAS in human whole blood and also discusses the challenges of PFAS analysis in blood serum and how to overcome them.

The Essentials of Air Sampling from Strategy to Solutions

On-demand webinar

Kristen Schultz (MilliporeSigma)

This webinar provides a workflow approach to guide the user from the strategy decision to the sample media selection process for air monitoring methods. The fundamental differences between active and passive sampling devices, when to use or not to use them noting the advantages and disadvantages of each type are outlined.

New ICP-OES Sample Introduction Components to Enhance Analysis of Challenging Samples

On-demand webinar

Eric Vanclay and Lindsey Whitecotton (Agilent)

Learn about new sample introduction components that can enhance analysis of challenging oil and organic solvents with improved productivity, better performance, and simpler maintenance routines.

Tips and Tricks to Improve your Titration Process

On-demand webinar

Bettina Straub-Jubb (Merck KGaA) and Thomas Hitz (Mettler-Toledo)

This webinar provides useful tips and tricks to improve your titration process and streamline your routine analysis. You will learn what parameters could influence your titration process and how to improve your daily workflow.

Mass Spectrometry to Fight Food Fraud

On-demand webinar

Chris Elliott (Queen's University Belfast), Chiara Cordero (University of Turin) & Stéphane Bayen (McGill University)

This expert discussion forum addresses some of the key analytical issues surrounding food fraud and authenticity testing.

The Value of Reliable Data: Leveraging LIMS Software to Support Compliance in your Food & Beverage Laboratory

On-demand webinar

Eugene Galperin (Thermo Fisher Scientific)

Watch this presentation to learn how Thermo Fisher Scientific’s SampleManager LIMS software can enhance laboratory processes in the F&B industry, focusing on reliable data.

Dilution and Sensitivity: Effects on a Food Contaminant Mega-Method

On-demand webinar

Dr Robert Di Lorenzo (Global Technical Marketing, Food & Beverage, SCIEX)

This presentation explores how far we can push dilution while still meeting sensitivity requirements to simplify sample preparation and analysis workflows. With the SCIEX Triple Quad™ 7500 LC-MS/MS System– QTRAP® Ready, a single mega-method comprising hundreds of pesticides, veterinary drugs and mycotoxins will be analyzed from various food and feed matrices from a single sample preparation method.

[LC Tutorials] How to Improve Method Robustness through Control of Unwanted Interactions

Online Training Tutorials

Kim Haynes (Waters)

This course is designed to take you from beginner to advanced in controlling the interactions within your chromatographic separations. It will cover how to improve method robustness through the prevention of analyte loss, with improved confidence in the quality of your data.

[LC Tutorials] Understanding LC Column Attributes - What's Important for Your Chromatography?

Online Training Tutorials

Jonathan Turner (Waters)

This course is designed to take you from beginner to advanced in understanding column selection and method development. It will cover the key aspects of the LC column from the base particle to the bonded phase, and even the hardware.

PFAS Testing Solutions & How Best to Minimize External Contamination Sources

On-demand webinar

Abderrahim Abdelkaoui (UCT)

This webinar provides solutions for PFAS testing in line with the updated method, EPA 533 and explains what measures can be taken to mitigate external contamination sources.

Fatty Acid Esters in Infant Formula - Determination of 2-MCPD and 3-MCPD Using an Agilent 8890 GC System with an Agilent 5977B GC/MSD

On-demand webinar

Youjuan Zhang (Agilent)

This presentation delivers a reliable analytical method for determining the fatty acid esters of 2-MCPD and 3-MCPD in infant formula. The presenter talks you through the method explaining how two different derivatization reagents, heptafluorobutyrylimidazole (HFBI) and phenylboronic acid (PBA), were evaluated for sample preparation.

What It Takes to Achieve a Good Chromatography – Selecting the Right Column Chemistries and Mobile Phase

On-demand webinar

Jonathan Turner (Waters Corporation) and Tony Edge (Hichrom)

This presentation will provide you with an understanding of different column particle technologies, their specific attributes and influences on chromatographic separation. You will also learn about the effect of mobile phase on selectivity and its use in method development.

How to Troubleshoot and Improve your GC/MS like an Agilent Scientist

On-demand webinar

Angie Smith-Henry and Vanessa Abercrombie (Agilent)

We’ll discuss how to use artifacts observed in chromatograms and tune reports to diagnose the problem, fix the problem, and 'future proof' your analysis with the best supplies, columns, and solutions.

LESS IS MORE: Microelution Extraction for High Throughput Bioanalytical Workflows

On-demand webinar

Stephanie Marin (Biotage LLC, Charlotte, NC, USA)

By watching this presentation you learn to appreciate the differences between microelution and traditional SPE products and protocols, describe processing conditions for manual and automated workflows for microelution columns, and compare protocols and results for a panel of steroids and a drug panel using traditional SPE columns and microelution columns.

Improving Energy Laboratory Productivity through Pre-configured Software Solutions

On-demand webinar

Eugene Galperin (Thermo Fisher Scientific)

Watch this on-demand presentation to learn how optimizing downstream processes, with SampleManager LIMS software Refinery Solution, can increase laboratory productivity in your refinery laboratory.

Strategies for Improving Sample Clean-up During the Analysis of Persistent Organic Pollutants (POPs) by Gas Chromatography

On-demand webinar

Deyny Mendivelso-Pérez (Senior R&D Scientist in the life science business of Merck KGaA, Darmstadt, Germany)

This on-demand presentation provides an alternative approach for the extraction of persistent organic pollutants (POPs) for gas chromatography analysis. Examples include analysis of pesticides using QuEChERS methodology and the use of unique solid phase extraction (SPE) cartridges for analysis of PCBs and PAHs in fatty foods.

Analytical Methodologies for Nitrosamines: Challenges and Solutions

On-demand webinar

Dr. BM Rao (VP & Head - CQC, ASAT & EM QA, Dr. Reddy's Laboratories Limited, Hyderabad, India)

By watching this presentation you will get background information on risk assessment and control of N-nitrosamine contamination in drugs, nderstand method development approaches and how to deal with the challenges, and learn to overcome analytical challenges presented by APIs and drug products.

Sample Preparation for Fatty and Complex Food Matrices

On-demand webinar

Klaus Buckendahl (Analytical Sciences Liaison, Advanced Analytical Europe, Merck KGaA, Darmstadt, Germany)

In this presentation, sample preparation tools based on zirconia-silica adsorbents for the QuEChERS methodology as well as in form of solid phase extraction (SPE) cartridges are introduced. Their applicability and improved fat and pigment removal is demonstrated for pesticide analysis in samples like avocado and spices, as well as for polycyclic aromatic hydrocarbons (PAHs) in edible oils.

Analysis of PFAS: Strategies to Optimize Performance

On-demand webinar

Detlef Knappe, Bradley Clarke & Carrie McDonough

This discussion forum addresses some of the key issues surrounding the targeted analysis of PFAS in environmental matrices. Benefit from the real-world experiences of our expert panelists who provide their insights into analytical approaches to the targeted determination and quantification of PFAS in environmental matrices.

Sample Preparation and Chromatographic Approaches to Quality and Sensory Testing of Beer

On-demand webinar

Klaus Buckendahl (Merck KGaA) & Nils Rettberg (Research Institute for Beer and Beverage Analysis (VLB))

This on-demand webinar comprises two presentations looking firstly at sample preparation tools for chromatographic testing of beer followed by flavour chemistry and sensory properties of non-alcoholic beer.

Perfecting Pcats – Developing a More Robust UHPLC-MS/MS Method for Plasma Catecholamines using Low Volume SPE

On-demand webinar

Adam Senior (Biotage GB Limited)

This presentation discusses typical plasma matrices and the effects of different sample pre-treatments. The importance of analyte and sorbent pH control is discussed along with selection of suitable approaches to maximize removal of matrix interferences and enhance method robustness. The final section covers different strategies for analyte enrichment to maintain method LOQs, using manual and automated low volume elution SPE procedures.

The Challenges of Pesticide Analysis - LC or GC Triple Quadrupole?

On-demand webinar

Bert Popping, Katie Banaszewski & Łukasz Rajski

Part 1 of this 'Instrument Selection Discussion Series' takes a look at the pros and cons of LC-QqQ and GC-QqQ for the analysis of pesticides in food and agricultural matrices. Industry and technology experts provide their insights on the various instrumental setups and workflows for analysing real-work samples.

Learn New Methods to Enhance Quantitative Workflows for VOC Analysis

On-demand webinar

Hannah Calder (Markes International) and Pascual Pérez Ballesta (European Commission, Joint Research Centre)

In this presentation we introduce the next generation in radial diffusive sampler – the POD Sampler. As part of this webinar you will find out how the POD samplers enhanced design can benefit your analytical workflow and field monitoring campaigns from its creator Pascual Pérez Ballesta.

Atomic Spectroscopy Learning Hub

Online Training Tutorials

Gareth Pearson & Eric Vanclay (Agilent)

Improve your analytical skills by visiting the ‘Atomic Spectroscopy Learning Hub’, a free-access portal produced by Separation Science. This portal is designed to enhance your knowledge, best practice and troubleshooting skills in the use of atomic spectroscopy techniques. Current and future course modules include ICP-MS and AA spectroscopy.

How Can LIMS Deliver Value to Your Pharma Manufacturing Lab?

On-demand webinar

Daren Cumberbatch & June Tham (Thermo Fisher Scientific - Digital Science)

This webinar focuses on how laboratory information management systems (LIMS) can deliver value and efficiency, and increase productivity, in your pharmaceutical manufacturing laboratory.

Connecting Performance Improvement to Increased Production in GC/MS Analytical Workflows

On-demand webinar

Angie Smith-Henry & Gustavo Serrano (Agilent)

This webinar goes over some of the Ultra Inert GC columns, liners and supplies, including gold-seals and novel gold-coated ferrules that are critical to achieve optimal performance even for low-trace level analysis and deliver consistent reliable results.

NPS Screening and Identification: Nominal and Accurate Mass Approaches for Ultimate Success

On-demand webinar

Pierre Negri (SCIEX)

The presenter discusses how nominal mass and accurate mass instruments can be used in forensic toxicology workflows as two different yet complementary approaches for screening and identifying novel psychoactive substances (NPS). The two different LC-MS/MS platforms are presented with an explanation of how they are used strategically for the detection and identification of NPS with a high level of sensitivity and specificity.

Key Notes on Citing Spectral Library Searches

Letter to the Editor

O. David Sparkman

A collection of mass spectra of known compounds and the program used to search a spectrum of an unidentified compound are two different entities. The results obtained depend on both. Full and correct citations should be the norm - find out how to do this correctly.

Latest Blog Posts

Food & Beverage Analysis Blog

Environmental Analysis Blog

Pharmaceutical Analysis Blog

Bio Clinical Analysis Blog

Liquid Chromatography Blog

Gas Chromatography Blog

Mass Spectrometry Blog

Stay ahead and improve your skills!

Follow us on Twitter: @Sep_Science

Be the first to know as our team will send straight to your inbox tailored information on new separation science methods and applications.