Separation Science (www.sepscience.com) offers free learning from the experts covering methods, applications, eBooks, webinars, eSeminars, expert forums, videos, tutorials and training for users of liquid chromatography, gas chromatography, mass spectrometry, spectroscopy, sample preparation and related analytical techniques.
Featured Educational Resources
Revealing the New Intelligent LC/MS Solutions from Agilent
Christian Klein and Patrick Batoon (Agilent Technologies)
Many labs are faced with an ever-increasing sample load, while instrumentation and resources remain at a steady level. As a consequence, instrument throughput and uptime is a critical factor to maintain competitiveness and productivity. In this webinar you will learn how you can meet these challenges as the presenters showcase the newest intelligent innovations in LC/MS, where high performance and data quality are matched with ease of use.
Karl Fischer Titration Week
19-22 June, 2023
Bettina Straub-Jubb, Shailly Krishna-Rajusth, Angela Schwehn (life science business of Merck KGaA, Darmstadt, Germany), Michael Margreth (Metrohm) and Thomas Hitz (Mettler Toledo)
Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices. This titration week covers different aspects that need to be considered when determining the water content in products, raw materials, and other substances. Experts from the life science business of Merck KGaA, Darmstadt, Germany, Metrohm AG, and Mettler Toledo will present and discuss different steps of the Karl Fischer titration process and how to improve it.
Selected Applications for Drugs of Abuse Analysis in Hair
Thomas Smith (Applications Laboratory Team Leader, Biotage)
The presentation highlights benefits of automation for laboratories with high throughput of samples and laborious manual methods, as well as challenges faced during analysis, including LOQs and matrix factors.
Enhancing the scientific experience using laboratory software
Nicola Gardner (Senior Manager, Product Marketing – Digital Science Solutions, Thermo Fisher Scientific)
Watch this presentation to learn about novel ways to interact with LIMS, delivering knowledge from data, automation solutions, and intuitive workflows.
Understanding Metabolic Pathways Using Mass Isotopologue Analysis with HILIC Zeno MRM High-Resolution
Luojiao Huang (Leiden Academic Centre for Drug Research, Leiden University)
In this presentation, you will see how a method that couples hydrophilic interaction liquid chromatography (HILIC) with Zeno trap-enabled high-resolution multiple reaction monitoring (MRMHR) was developed in order to acquire the isotopic labeling information at the intact metabolite and moiety level simultaneously.
GC Solutions for Hydrogen Impurities Analysis
Youjuan Zhang (Applications Chemist, Agilent Technologies, Gas Phase Separations Division) and Scott Hoy (Applications Chemist, Agilent Technologies, Gas Phase Separations Division)
By watching this presentation you will learn about regulatory considerations for high-purity hydrogen impurities, different GC platforms for hydrogen impurities analysis, and advantages of different GC configurations.
Fast and Selective Purification of Oligonucleotides
Video & Application Note
Florian Rieck (Agilent Technologies)
Join Dr Florian Rieck in a short presentation as he introduces a method for the fast and selective purification of oligonucleotides. The complementary application note, which is available for download, describes a purification method with MSD that uses alternative, less costly reagents. Target compounds were collected faster and with higher purity compared to conventional methods.
Powering through the complexity of elemental environmental analysis
Jon Peters and Dr. Daniel Kutscher (Thermo Fisher Scientific)
This presentation explores the role that ICP-MS and supporting technologies play in environmental analysis. Learn how these latest technologies can simplify your workflow and increase uptime and productivity whilst discovering how automated sample handling systems can support robust instrument performance when analysing difficult environmental samples.
EXPERT ROUNDTABLE: Why is PFAS testing so challenging?
Lee Ferguson, John Quick, Vladimir Nikiforov, Christopher P. Higgins and Ed George
This exclusive roundtable event hosts experienced industry leaders in the PFAS analytical testing field to share their own experiences and discuss various topics that help empower PFAS analytical testing laboratories to future-proof themselves, in this constantly evolving area of environmental concern.
How to Go Fast and Make your Carrier Gas Last
by Vanessa Abercrombie and Samuel Haddad (Agilent Technologies)
This presentation goes over tips and tricks on how to speed up your overall GC(MS) analysis method, to help ensure high sample throughput and maintain data integrity. It discusses how to properly transition your method from helium to hydrogen carrier gas, along with consumables that you may want to consider.
The Smart Behind Separations: Enabling a New User Experience for GC and GC/MS Workflows
Alessandro Baldi Talini and Tiffany Arbon (PerkinElmer)
Discover how your lab can benefit from innovative technology that enables access to real-time information on the go. Learn what the 'smart behind separations' is that enables smart connectivity and simplified, sustainable operations for GC and GC/MS; and how intuitive CDS software can help reduce technology adoption costs.
Computerized System Validation: GAMP 5 2nd Edition, What You Need to Know
Michael Korbel (Global Sr. Product Manager, Agilent Technologies, Inc.)
This presentation takes a look at the contents of the long awaited GAMP®5 Version 2 release to examine what has truly changed since the Version 1 release, with special focus on what might impact regulated laboratories in the pharma, biopharma, and biotech industries.
Evolution of USP<1058> updates and insights in regulations
Paul Smith (Agilent Technologies, Inc.)
Many laboratories are not currently complying with the 2017 edition of USP <1058> and plans are already in progress to initiate further updates. This presentation addresses many of the questions about the role of the current <1058> general chapter and how it aligns with the recently issued 2nd Edition of GAMP® 5.
(U)HPLC Analysis of Biopharmaceuticals: Quality Attributes and How to Analyse Them
Andrea Krumm (Tosoh Bioscience)
In this tutorial series you will learn the basics of (U)HPLC analysis of biotherapeutics, such as monoclonal antibodies and other proteins. We explain which critical quality attributes need to be analyzed and which (U)HPLC methods are suitable for these purposes. In addition, we provide tips and tricks from our chromatography experts for successful method development.
Indoor Air Quality – The Science and Regulations Behind the Headlines
Caroline Widdowson (Markes International) & Thomas Neuhaus (Eurofins Product Testing)
This presentation addresses the wide range of regulatory and market pressures placed on manufacturers to limit the chemical emissions from products and materials. It describes the use of thermal desorption–gas chromatography–mass spectrometry (TD–GC–MS) to characterise the chemicals that affect indoor air quality, and highlights the importance of robust sample preparation, sampling and analytical procedures.
LC-MS/MS Fundamentals: The Overview
by Jack Steed and Jessica Smith (SCIEX)
This presentation discusses the fundamentals of LC-MS technology and how this can be utilized in your everyday workflows. The discussion ranges from the basics of LC separations to the implementation of high-resolution mass spectrometry, including data acquisition and review.
Learn how Intelligent Software can Streamline your Elemental Analysis Workflow
Jon Peters and Shona McSheehy Ducos (Thermo Fisher Scientific)
This presentation highlights the key role that software plays in a modern and integrated laboratory for elemental analysis. Watch and learn how software can support daily tasks and streamline your analytical workflow.
How to optimise your PFAS air sampling method using thermal desorption
Helen Martin and Hannah Calder (Markes International)
This presentation discusses the advantages that thermal desorption offers, how to optimise a system for targeted and untargeted PFAS analysis, and how to overcome challenges posed by low-level PFAS monitoring.
How LIMS can benefit battery manufacturing workflows
Tareq Al-Najjar (Global Strategic Account Executive, Energy – Digital Science Solutions, Thermo Fisher Scientific)
This presentation outlines the benefits of implementing a laboratory information management system into battery cell manufacturing workflows. You will discover how implementing a LIMS into your battery manufacturing organization can aggregate data in a meaningful way, seamlessly share that data, and drive insightful decision-making.
Rethinking Routine Analytical Workflows through Volume Reduction and Solvent Consumption
Amber Cain (Application Scientist, Biotage)
Presented here is a brief overview of common but essential workflows in a microelution format, fully automated on the Biotage® Extrahera LV-200 sample preparation instrument. Workflows are simple, cost effective, and include discussions of drugs of abuse, steroids, catecholamines, and metanephrines.
Accelerating Large-scale Proteomics with High-throughput Accurate Mass Spectrometry Workflows
Dorte Bekker-Jensen (Evosep Biosystems) and Patrick Pribil (SCIEX Proteomics Applications Development)
This presentation discusses high-throughput mass spectrometry workflows for large-scale qualitative and quantitative proteomics. It explores the rapid adoption of data-independent acquisition (DIA or SWATH DIA) approaches, such as Zeno SWATH DIA: a method that achieves 5-6x more MS/MS sensitivity, resulting in this DIA approach surpassing data-dependent acquisition (DDA) for protein identifications and quantification in complex matrices.
[Application] Analysis of Lipid Nanoparticle Composition
Dr. Sonja Schneider (Agilent Technologies)
This application note describes the analysis of the lipid components of patisaran using an Agilent 1260 Infinity II Prime Bio LC system and evaporative light scattering detection (ELSD). Read the application to learn about composition and structure of lipid nanoparticles, how to achieve best LC separation for the LNP components, and how to use the ELSD for universal detection.
Critical Insights in the Detection of PFAS in Our Environment
Derek Mattern (PerkinElmer)
This presentation explores some high-performance solutions to optimize your laboratory workflow for PFAS analysis. It provides a detailed overview of PFAS LC/MS/MS methods, including direct injection and SPE methods fitting to various global regulations.
Biphenyl, the Go To Phase for LC-MS Method Development
Paul Connolly (Senior Product Manager for Liquid Chromatography, Restek)
Learn about the mechanisms of retention of the Biphenyl phase; best practices for successful Biphenyl utilization in liquid chromatography methods; and see examples of Biphenyl chromatography with applied solutions for multiple market needs. In addition, get an introduction to the ProEZLC Modeller for method development.
Carboxen® Synthetic Carbon: A Derivatized Resin for Process and Preparative Chromatography
Joe Abrahamson (MilliporeSigma)
Discover the features, advantages and benefits of synthetic carbon separation media in both liquid and gas purifications and how they bring value to a wide range of LPLC applications for both small and large molecule separations.
Helium Shortage 4.0: Is hydrogen carrier gas the solution to the GC–MS problem?
Diane Turner (Anthias Consulting Ltd), Chris Siegler (Dow Inc.), Helen Martin (Markes International) and Matthew Klee (XO Associates LLC)
In this on-demand presentation and discussion forum, our panel of experts talk about the pros and cons of using hydrogen as an alternative carrier gas and address audience questions.
Advance Your Titration Process — Ensure Data Integrity and Correct Results
Bettina Straub-Jubb (Merck KGaA, Darmstadt, Germany) and Thomas Hitz (Mettler-Toledo)
Watch this presentation to gain a deeper understanding of how to advance your titration process using new technologies for fast and safe data integrity, meet quality assurance aspects, comply with regulations and become more sustainable.
Carrier Gas Selection in Gas Chromatography
Daniela Cavagnino (Thermo Fisher Scientific), Ian Parry (Thermo Fisher Scientific) and Ed Connor (PEAK Scientific)
By watching this expert discussion you will learn about carrier gas choices for GC, benefits and limitations of using hydrogen versus helium, benefits of gas generators versus other gas sources, what is involved in converting from helium to hydrogen in the laboratory, user benefits that can be realized and innovative solutions that can be expected in GC in the future.
Valisure’s Identification of Benzene as a Pervasive Contaminant in the Consumer Products Industry
David Light and Kaury Kucera (Valisure)
This presentation describes the identification of benzene in a variety of consumer products, such as hand sanitizers, sunscreens and body sprays and how Valisure provides independent quality assurance throughout the supply chain.
The Power of Data Analytics: Driving Laboratory Efficiency and Discovering New Insights
David Hardy (Thermo Fisher Scientific)
Discover the benefits of data analytics and visualization tools, and learn how laboratories can effectively use these to optimize their data to drive efficiency and innovation.
Advantages of Laminar-Flow Mass Spectrometry for a Sensitive and Robust Determination of Mycotoxins in Food
Francisco Ferron (Senior Product Specialist for LC-MS, PerkinElmer)
The presentation introduces you to the most advanced methods for food contaminants determination. In particular, performances of the LC-MS/MS technology and Laminar-Flow Mass Spectrometry applied to food analysis and strategies for method optimization are covered.
New Methodology for the Analysis of Pesticides in Fruits and Vegetables by GC-HRMS
Ramkumar Dhandapani (Phenomenex) and Javier Arrebola (University of Almería)
Discover some invaluable method optimization tips and tricks for pesticide analysis of real sample matrices, including fruits and vegetables, by gas chromatography/high-resolution mass spectrometry (GC-HRMS). Learn how the use of HRMS is increasingly being incorporated to routine work-flow laboratories because of its power to discriminate analytical information and capability to avoid matrix interferences and how it also helps to evaluate the performance of the tested GC column.
Global PFAS Testing Virtual Symposium: Advances in Occurrence & Testing
Frederik Zietzschmann (Laboratory of Berliner Wasserbetriebe), Stephen Somerville (Pace Analytical), Dr. Nobuyoshi Yamashita (AIST), Dr. Bradley Clarke (University of Melbourne), Dr. Arjun Venkatesan (Stony Brook University), Dr. Erin Baker (North Carolina State University)
Learn about the latest advances in quantification of legacy and novel PFAS; developing and accreditation for routine and regulatory PFAS methods including EPA and EU guidelines; novel analytical techniques to identify new PFAS; and testing methods optimized for water, air, commercial products, textiles, soil and others.
Introducing Agilent’s CrossLab CS Electronic Leak Detector and ADM Flow Meter: Two GC Diagnostic Tools, One Convenient Package
Paul Tripp and Angela Smith-Henry (Agilent)
Leaks are problematic for any operation that uses gas tubing and fittings. Even small gas leaks can lead to safety issues, reduced productivity, and increased costs. Here the presenters introduce the CrossLab CS Electronic Leak Detector and ADM Flow Meter — two GC diagnostic tools in one cost-effective, convenient package.
Powering Up Battery Materials Analysis using ICP-OES and ICP-MS
Simon Nelms and Sukanya Sengupta (Thermo Fisher Scientific)
This presentation provides a complete overview of how ICP-OES and ICP-MS analysis supports every step in the battery manufacturing value chain, from raw material screening and refining, through battery cell material research and QA/AC, to recycling and environmental analysis. The basics of ICP-OES and ICP-MS technology are explained and selected analysis examples demonstrated.
Practical Challenges in PFAS Extraction for Water Samples and How to Address Them
Bill Jones (Director of Chemical R&D, Biotage LLC)
This practical, experience-based webinar is useful for anyone involved in or performing method development for PFAS in aqueous matrices using a range of methods. You will gain more information on the pervasiveness of PFAS compounds in a typical extraction laboratory and the steps that can and should be undertaken to combat them.
Optimized Workflow of POPs Analysis in Environmental and Food Matrices Using Semi-Automated Cleanup and GC-MS/MS
Ruud Addink (Technical Director, Fluid Management Systems)
This presentation discusses several clean-up methods with data and shows how the Agilent 7010 GC-MS/MS instrument can be used for state-of-art dioxin and PCB work at levels of sensitivity equal to or better than magnetic sector instruments.
Electron Activated Dissociation (EAD) MS/MS for the Structural Elucidation of Lipids
Sophia Laposchan (Technical University of Munich, Germany)
By viewing this presentation you will gain a basic understanding of using electron activated dissociation in MS/MS analysis for structural elucidation of lipids; and learn how EAD can distinguish structural isomers of various lipid classes and how this powerful technique adds value over other fragmentation techniques.
Knowing How to Make ICP-MS Easier
Gareth Pearson (Agilent, Spectroscopy Technology Innovation Centre, Melbourne, Australia), Glenn Woods (Agilent, Centre of Excellence, Manchester, UK), and James Dellis (Agilent, Spectroscopy Technology Innovation Centre, Melbourne, Australia)
Learn how Easy-fit supplies can simplify your workflow, discover tools that can help you get your ICP-MS configured right for your analysis, take a look at ICP-MS MassHunter software, and learn about some of the simple software tools and time-saving tips that can help you get the very best out of your ICP-MS.
Gas Chromatographic Analysis of Biofuels – A Survey of Solutions to Assure Quality and Performance for Today’s Energy Needs
Jim McCurry (Senior Application Chemist, Agilent)
Several gas chromatographic (GC) methods have been developed to help producers and users of biofuels meet these specifications. This seminar presents solutions built on Agilent’s Smart Gas Chromatographic instruments. Analysis of several classes of biofuel samples demonstrates the performance of these solutions in meeting the requirement of industry standard methods.
Detection and Quantification Challenges of Trace Level Impurities in Pharmaceutical Products - Mutagenic Nitrosamines and Beyond
Multi-speaker event (Novartis, EMD Serono, AstraZeneca, US Pharmacopeia) sponsored by VUV Analytics
This on-demand discussion forum covers the risk assessment and analytical challenges of detection and quantification of trace level impurities in pharmaceutical products.
PAMS Analysis during COVID-19 Lockdowns in New York City
Pete Furdyna (Applied Analytical Chemist, NYSDEC, USA) and Laura Miles (Senior Application Chemist, Markes International, UK)
In this presentation you will learn how NYSDEC achieved excellent data stability over extended sampling periods, how water can be effectively managed for sample integrity and to achieve sub-ppb levels of detection for ozone precursor VOCs, and the benefits of using hydrogen carrier gas for continuous, robust and unattended monitoring in remote locations.
Ask the Agilent Experts - Part 2: GC and GC/MS Scientists Answer Your Most Frequently Asked Questions
Angie Smith-Henry & Vanessa Abercrombie (Agilent) with Diane Turner (Moderator - Anthias Consulting)
This follow-up presentation in the ever popular 'Ask the Agilent Experts' series continues to discuss some of the most frequently asked questions about GC and GC/MS. You will learn best practices with GC and GC/MS systems, and discover why there is not always an easy answer to most GC(MS) questions.
Leveraging Integration: The Advantages of using a LIMS with your CDS
Katie Evans (Thermo Fisher Scientific)
This presentation describes the benefits of connecting your laboratory information management system and your chromatography data system. You will learn how the integration between the two can streamline workflows and enhance efficiency in your lab.
Determination of Anions and Cations in Power Plant Water using Ion Chromatography
Peter O’Brien (EDF Energy, UK)
Learn how ion chromatography with suppressed conductivity detection is the safe and reliable method of choice to determine both µg/L (ppb) and mg/L (ppm) levels of anions and cations including transition metals in power plant waters.
Dissolution Today, Tomorrow & Beyond
Vivian Gray, Lee Ashworth, Samir Haddouchi, Andre Hermans & Bryan Crist
This expert presentation and discussion forum focuses on aspects of dissolution from development through to a marketed product. Our expert panellists discuss the role of dissolution as a quality assurance tool for product development, approval and batch release, the use of dissolution testing of novel drug formulations and how it is being used to probe dosage form behaviour.
Ask the Experts: Which Trace Elemental Instruments are Best for your Analysis?
This is the second on-demand instalment of the 'Instrument Selection Discussion Series' where laboratory professionals gain knowledge on the choices of instrument available and which is most recommended for specific applications.