A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. UHPLC (ultra-high pressure LC) is rapidly becoming a tool used by many laboratories to speed up the development and application of chromatographic methods. We compare UHPLC and HPLC in terms of potential strengths and weaknesses.
This course is designed for practical workers in the laboratory who have the responsibility for designing new HPLC or UHPLC methods or transferring methods between laboratories. Experienced workers will get the most out of this class, but because it builds from the fundamentals, anyone with experience in HPLC or UHPLC will gain valuable knowledge.
This course covers:
UHPLC (ultra-high pressure LC) is rapidly becoming a tool used by many laboratories to speed up the development and application of chromatographic methods. We compare UHPLC and HPLC in terms of potential strengths and weaknesses. UHPLC methods can be more demanding on the system hardware, so we examine the different aspects of the hardware that must be attended to. UHPLC has a reputation of being a more difficult technique to use than HPLC – some of this is due to the technique itself and some are due to the requirement of more care by the operator. We examine some of the most common problems and how to avoid them. When moving methods between HPLC and UHPLC, proper scaling is important, so we discuss how to scale a method and give a practical example of this.
- The Advantages of UHPLC
- Possible Surprises (in two parts)
- Scaling Separations
By attending this online training course you get full access to the 38 video modules and approximately 12.5 hours of instruction. You also get handouts containing copies of all of the approximately 520 PowerPoint slides used in the class. These are arranged for easy note-taking while you view each module and give you a valuable resource for future reference.
This module has a complementary quiz. By correctly answering the quiz questions for all of the modules as part of the course you will be able to download your certificate of completion. Types of questions to expect are:
- What are the advantages of UHPLC over HPLC?
- Why is UHPLC more powerful than HPLC for method development?
- Why should superficially porous (SP, fused core, pellicular) particles be considered as possible alternatives to totally porous (TP) sub-2 micron UHPLC particle columns?
- What can be said about the influence of dwell volume (instrument delay volume) with UHPLC?
- What can be said about the detector cell volume and extra-column effects in UHPLC?
- What can be said about thermal effects with UHPLC and sub-2-micron columns?
- What can be said about the influence of the pressure with UHPLC?
- How does the mobile phase differ in UHPLC systems?
- Why is more care required when handling UHPLC mobile phases and samples than for conventional HPLC applications?
- When scaling an isocratic separation from HPLC (e.g., 5 µm particles) to UHPLC (e.g., sub-2-µm particles), what precautions should be taken?
- When scaling a gradient separation from HPLC to UHPLC, what precautions should be taken?
- What is the difference comparing scaling for isocratic and gradient separations from HPLC to UHPLC?
- A change in which of the following parameters in a gradient requires some compensatory adjustment to keep selectivity constant?
Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique education resource for analytical chemists. Currently, we offer HPLC training, LC-MS training, GC training and GC-MS training.