A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We examine the processes that influence band spreading and how columns have evolved to their present configuration. The choice of the column can be one of the most important decisions when developing a separation, so we go into detail about the chemistry of the silica support and bonded phase.
This course is designed for practical workers in the laboratory who have responsibility for designing new HPLC or UHPLC methods or transferring methods between laboratories. Experienced workers will get the most out of this class, but because it builds from the fundamentals, anyone with experience in HPLC or UHPLC will gain valuable knowledge.
This course covers:
We examine the processes that influence band spreading and how columns have evolved to their present configuration. The choice of a column can be one of the most important decisions when developing a separation, so we go into detail about the chemistry of the silica support and bonded phase. The different bonded phase types are reviewed.
- Packing Characteristics
- Alkylsilica Phases
- Other Reversed-Phase Options
By attending this online training course you get full access to the 38 video modules and approximately 12.5 hours of instruction. You also get handouts containing copies of all of the approximately 520 PowerPoint slides used in the class. These are arranged for easy note-taking while you view each module and give you a valuable resource for future reference.
This module has a complementary quiz. By correctly answering the quiz questions for all of the modules as part of the course you will be able to download your certificate of completion. Types of questions to expect are:
- What are the main differences between today’s columns vs. those columns 20 or 30 years ago?
- What is the difference between smaller particles?
- What does a van Deempter plot show?
- How does the column plate number, N, change with packing particle diameter, dp?
- How does column backpressure change with packing particle diameter?
- What advantage do modern superficially porous (fused core, core-shell, pellicular) particles, SP, have over totally porous, TP, particles as a column packing?
- How does silica purity affect the appearance of the chromatogram?
- What is bonded phase columns?
- What is the stability of alkyl bonded phase columns at low pH (e.g., <2)?
- When a column is described as a “fully bonded” C18 column, what does this mean?
- How does silica purity affect column performance?
- What are the factors controlling selectivity (peak spacing) in reversed-phase HPLC?
- What are embedded polar group (EPG) or “aqueous” (AQ) columns?
- What do you expect to observe when you change to an “orthogonal” column, such as from a C18 to an EPG column?
- What are the important factors when choosing a column?
Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique education resource for analytical chemists. Currently, we offer HPLC training, LC-MS training, GC training and GC-MS training.