Systematic chromatography method transfer

by | Mar 9, 2017

On-demand Webinar to learn systematic protocols for (U)HPLC and UPLC chromatography method transfer and the separation challenges such as system dwell volume, extra column dispersion, temperature effects,...

Successfully transferring a method to a different instrument or laboratory is a challenge faced by most chromatographers. Method transfer might be as simple as migrating an established method to an identical instrument keeping all separation parameters constant — or it may involve adapting the method to a new instrument with an “equivalent” column or attempting to substantially improve the method for a more modern instrument with differences in column chemistry.


Systematic success
The path to success requires the analyst to apply a systematic protocol based on an understanding of fundamental chromatographic principles and potential errors.

TomWheat_Work.pngThomas E. Wheat of Waters Corporation, in conjunction with Separation Science, presents an informative webinar on the principles of developing and validating chromatography method transfers.

You will learn

  • How to successfully transfer established chromatographic methods
    • to a new user or laboratory
    • to a different instrument with the same colum
    • to an instrument using a newer separation mode such as UHPLC or UPLC
  • The separation challenges in method transfer, such as system dwell volume, extracolumn dispersion, temperature effects, injection mode, and detector considerations
  • Potential pitfalls
  • Factors required for successful validation of transferred methods
  • The role of information-rich detectors in confirming a successful transfer

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