This article discusses the most popular detection principles in use in industry and CE-related good working practice.
Previously we went through the fundamentals, the separation medium BGE, the capillary and injection in capillary electrophoresis. In this article we will reach the end of the system, the detector. We will discuss the most popular detection principles in use in industry and CE-related good working practice.
The usual detection techniques for liquid separation are also available for CE and UV detection is by far the most common. Modern equipment comes with build-in UV/VIS single wavelength or diode array detectors, specially designed to focus the beam of light into the capillary. Absorption is a function of the path length (Lambert-Beer’s law). As we detect on-column in CE, the path length is directly related to the capillary diameter. Compared to other liquid separation techniques, this means that the path length is rather short, which limits detection sensitivity. The loss in sensitivity from the path length is partly compensated for by the high efficiency in CE and by the possibility to use low UV wavelengths.
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