Reference Guide

GC/MS/MS Pesticide Residue Analysis

Discover practical tips and considerations for pesticide multiresidue analysis using GC/MS/MS.

This reference guide provides examples of recommended, proven, and robust gas chromatography/tandem mass spectrometry (GC/MS/MS) methods for pesticide multiresidue analysis using Agilent triple quadrupole GC/MS/MS systems. The majority of pesticide laboratories have transitioned from GC/MS to GC/MS/MS techniques due to enhanced sensitivity and selectivity, especially in the presence of coeluting matrix interference. Included in this guide are practical tips and considerations for method development, optimization, modification, and routine use. Important aspects of GC and MS/MS analysis of pesticides are discussed, especially when dealing with matrix-related issues that are largely affected by the chemical composition of the analyzed sample extract.

The basics of sample preparation in pesticide multiresidue analysis are also covered with a focus on the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) approach. Additional topics include the best practices for setting up and configuring a triple quadrupole GC/MS system (GC/TQ) for hydrogen carrier gas, dMRM/scan acquisition mode, and advances in software tools, such as the Agilent MassHunter Optimizer for GC/TQ.

Four GC/MS/MS methods are included in Annex IV to Annex VII at the end of this guide. The first two methods (Annex IV and Annex V) are legacy methods used by the authors as a reference for method development and optimization. The second two methods (Annex VI and Annex VII) present the recommended instrument configuration, GC columns, consumables, and method parameters for the best method performance with helium and hydrogen as carrier gases.

This 110-page reference guide is divided into the following topics:

  • Pesticide multiresidue analysis
  • Sample preparation using the QuEChERS approach
  • Use of internal and quality/process control standards
  • GC analysis of pesticides
  • MS/MS detection considerations
  • GC/MS/MS method development and optimization examples
  • GC/MS/MS method modification for a different list of analytes
  • GC/MS/MS routine analysis

Plus access the bonus content:

[Application Note] Brewing Excellence: Quantitating Over 200 Pesticides in Black Tea with Steady Performance and Maximized Uptime by GC/MS/MS
This application note presents results for the sensitive and robust quantitation of 246 pesticides in black tea extract with the Agilent 7010D Triple Quadrupole Mass Spectrometer (GC/TQ) featuring a second-generation High Efficiency Source 2.0 (HES 2.0) that addresses the challenges posed by residual pesticide analysis in complex matrices.

[Application Note] Enhanced Longevity and Revolutionized Robustness for the Sensitive Detection of 190 Pesticides over 800 Injections
This application note highlights the use of the Agilent 8890 gas chromatograph (GC) coupled with the Agilent 7010 triple quadrupole mass spectrometer (GC/TQ) with a high efficiency source 2.0 (HES 2.0) upgrade as an analytically accurate, robust, and reproducible instrument for the multiresidue pesticide analysis of complex samples.

[Application Note] Quantification of Nitrosamine Impurities in Sartan Drugs Using an Agilent GC/TQ with Hydrogen Carrier Gas
This application note shows the suitability of hydrogen as a carrier gas and compares it with helium for the analysis of eight nitrosamine impurities in certain sartan drugs using either an Agilent 8890 gas chromatograph system coupled to an Agilent 7010 Series
triple quadrupole (TQ) GC/MS system with a high-efficiency ion source (HES), or an Agilent 7000E triple quadrupole GC/MS with an Agilent HydroInert ion source.

[Product Video] High-Performance GC/TQ Goes Beyond All Limits
The Agilent 7010D GC/TQ features a novel HES 2.0 ion source that delivers attogram-level sensitivity and industry-leading robustness, ensuring exceptional instrument uptime that directly contributes to a rapid return on investment. Powered by the new MassHunter Acquisition 13.0 and built-in intelligence capabilities, this instrument is designed to streamline laboratory workflows and meet evolving regulatory demands.


The information you provide will be shared with the sponsors of this content. Separation Science or its sponsors may contact you to offer you content or products based on your interest in this topic. You may unsubscribe from these communications at any time. For information on how to unsubscribe, our privacy practices, and commitment to protecting your privacy, check out our Privacy Policy.