How to improve method robustness through control of unwanted interactions

by | May 11, 2022

Learn about the fundamentals and best practices of HPLC, from the column perspective, through this open-access liquid chromatography training course by Waters.

Successful chromatographic separations are as much about preventing the interactions you don’t want, as it is about creating the interactions you need. This open-access course is designed to take you from beginner to advanced in controlling the interactions within your chromatographic separations. It will cover how to improve method robustness through the prevention of analyte loss, with improved confidence in the quality of your data.


The full "How to improve method robustness through control of unwanted interactions" open-access course includes six tracks, including:

  • Introduction to non-specific binding and non-specific adsorption
  • Common approaches and newer innovations to prevent non-specific adsorption
  • Improving reproducibility and reducing column conditioning through control of non-specific adsorption
  • Improving sensitivity and peak shape through control of non-specific adsorption
  • Column Selection in a world with non-specific adsorption
  • Simplified Method Development with Risk Mitigation against Non-Specific Adsorption

Once you complete all the tutorials in this module, including answering all the quiz questions correctly, you will receive a certificate of completion.

The Presenter

Kim Haynes is Director, Chemistry Marketing and Product Management in the Chemistry Technology Center at Waters Corporation.

Kim manages MaxPeak High-Performance Surface Technologies as well as a variety of other programs. Prior to this position, she worked with Waters Corporation as a chemistry sales specialist and a business development manager. During her 21 years at Waters, Kim has focused on helping scientists and colleagues understand chromatographic separations and sample preparation. Before joining Waters Corporation, Kim worked as an analytical chemist at Bacardi Martini. She holds a BS in Chemistry from Ohio University.

LC Tutorials by Separation Science

Now that you’ve learned about controlling unwanted interactions, be sure to check out our other modules:

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