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HPLC Training

Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practices, troubleshooting and method development training for chromatographic and mass spectrometric techniques.

Comprehensive, self-paced online courses and validated learning provides a unique educational resource for analytical chemists. Currently, we offer HPLC training, LC-MS training, GC training and GC-MS training.

Self-paced Validated Learning
All courses are created by analytical training experts and segmented in a logical way to ensure the most effective educational experience. All course modules are supplemented by quiz questions to validate your learning.
Certificate of Completion
Users can monitor progress via their ‘My Account’ page. Once all the course modules have been viewed and all quiz questions answered correctly, users will be able to download a course completion certificate.

Cost-Effective Learning
Course purchase provides 12 months of unlimited access to expert-developed learning materials, course monitoring and a certificate of completion. Prices range from US$ 189 – US$ 359 per course (except for ‘Interpretation of Mass Spectra’).

Looking for Cost-effective Online Learning in LC?

Looking for Cost-effective Online Learning in Advanced HPLC Method Development and HPLC Validation?

Does your site have multiple users who would benefit from expert online training in LC method development and validation?

Advanced HPLC Method Development
Learn a time-proven technique that walks you through the method development process using sound chromatographic principles.

Principles of HPLC Validation
Validate HPLC methods for the analysis of pharmaceutical drug substances, drug products, impurities, and degradation products.

  • For a site licence, Analytical Training Solutions provides unlimited access for ALL of your users* to both the ‘Advanced HPLC Method Development’ and ‘Principles of HPLC Validation’ courses for a 12-month period.
  • Both courses are broken down into manageable modules and presented in a logical manner for effective learning.
  • Each video module is accompanied by a short quiz to validate your understanding of the materials.
  • Upon completion of a course, each user will be able to download a ‘Certificate of Completion’.

For more information complete this form or contact: dean.graimes@sepscience.com.

* Note: all users must be based at the same geographic site.

Liquid Chromatography (LC) Training

Fundamentals of HPLC

Self-paced online course with validated learning and certificate of completion

Analytical Training Solutions | Separation Science

‘Fundamentals of HPLC’ covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation, including: The basics of the chromatographic process, Terms and definitions of chromatography, How HPLC systems work, Practical tips on preventive maintenance, Principles of separation chemistry, Why “reversed-phase” is the standard separation technique, The meaning of “gradients” and why they are used, How to get the best quantitation and much more. By taking this online course you will acquire a good understanding of the fundamentals of HPLC and get a better knowledge of the key concepts involved. The course is aimed at managers, supervisors, auditors, reviewers, etc. who use information generated by HPLC but do not necessarily do chromatography themselves.

HPLC & UHPLC Troubleshooting

Self-paced online course with validated learning and certificate of completion

Analytical Training Solutions | Separation Science

'HPLC & UHPLC Troubleshooting: A Performance Qualification Approach' is an intensive online course that teaches you the ins and outs of solving problems that occur with your high-performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) methods. You will learn how to qualify the performance of your HPLC or UHPLC using specific tests that also can be used for isolating existing problems. More importantly, you’ll learn how to prevent many of these problems from happening in the first place. This course is designed for anyone who works with HPLC or UHPLC. No previous experience with HPLC or UHPLC systems is assumed; however, much of the course will appeal to the more experienced worker.

Advanced HPLC Method Development using QbD

Self-paced online course with validated learning and certificate of completion

Analytical Training Solutions | Separation Science

'Advanced HPLC Method Development Using Quality by Design' is an intensive online course that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods. Here’s what the course covers: What Quality by Design (QbD) is and how it applies to HPLC methods?, A review of basic HPLC theory as it applies to method development?, The makeup of HPLC columns including the support material and the bonded phase?, The role of solvents in the separation?, How the column plate number, selectivity, and retention can be used in a logical way to solve even the toughest separation problems?, How to use mobile phase solvents, pH, temperature, chemistry, and other variables to pull apart difficult-to-separate peak pairs?, How gradient elution works and how to remove the mystery of optimizing gradients?, How a scouting gradient can be used to speed the development of both isocratic and gradient methods? How to use a simple spreadsheet calculator to move from the scouting run to the next step in method development?, How computer-assisted method development tools can leverage a small amount of data into a large amount of information about a separation?, How to take advantage of a publicly available internet database to identify equivalent and orthogonal columns?, When standard reversed-phase techniques don’t work, how to choose mixed-mode, ion-pairing, normal phase, HILIC, chiral, or other chromatographic modes?, What’s the same and different when comparing HPLC and UHPLC?, What to look for when validating a method?

Principles of HPLC Validation

Self-paced online course with validated learning and certificate of completion

Analytical Training Solutions | Separation Science

This class focuses on validating high-performance liquid chromatographic (HPLC) methods developed for the analysis of pharmaceutical drug substance (pure drugs), drug product (formulated drugs), impurities, and degradation products. Although the focus is on pharmaceuticals, the principles apply for most quantitative HPLC methods, such as environmental or general chemical analyses. Here’s what the course covers: What steps are that need to be undertaken during validation?, Why quality is so important?, What basic chromatographic measurements will be used?, Why to approach validation as part of a larger process?, Which regulatory documents are important?, What parameters of the method need to be tested?, What are the different standardization techniques, and when are they used?, What is Quality by Design, and why is it important?, What software tools are available to simplify the process?, What pitfalls should be avoided in validation?, What is pre-validation, and why is it worth my time?, How do I choose a system suitability sample?, How can I tell if the method is out of control?, How much can I adjust a method before I have to re-validate?

HPLC for Biopharmaceuticals

Self-paced online course with validated learning and certificate of completion

Analytical Training Solutions | Separation Science

Although “small molecule” and “biopolymer” separations have traditionally been considered as separate activities, analysts in the biopharmaceutical industry regularly have to deal with both. Fortunately, the underlying principles of chromatography apply equally well in both situations when interpreted appropriately. 'Practical HPLC for Biopharmaceuticals' explains chromatography in practical terms from the ground up. Here’s what you’ll learn in this online course: The basics of isocratic and gradient HPLC, Reversed-phase and ion exchange HPLC, HPLC equipment – from reservoirs/pumps to tubing/injectors to columns and detectors, Understanding and overcoming common column problems, Alternative modes: normal phase, HILIC and size exclusion, Quantitation, UHPLC, Performance qualification, Troubleshooting and diagnostics.

LC-MS Training

LC-MS/MS for Chromatographers

Self-paced online course with validated learning and certificate of completion

Analytical Training Solutions | Separation Science

With the introduction of the mass spectrometer (MS) as a practical detector for high-performance liquid chromatography (LC or HPLC), LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector. You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques and focus the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.