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Methods & Applications
Separation Science offers free learning from the experts covering methods & applications and eBooks for users of liquid chromatography, gas chromatography, mass spectrometry, spectroscopy, sample preparation and related analytical techniques.
Featured Methods & Applications
Fast and Selective Purification of Oligonucleotides
Video & Application Note
Florian Rieck (Agilent Technologies)
Join Dr Florian Rieck in a short presentation as he introduces a method for the fast and selective purification of oligonucleotides. The complementary application note, which is available for download, describes a purification method with MSD that uses alternative, less costly reagents. Target compounds were collected faster and with higher purity compared to conventional methods.
Analysis of Lipid Nanoparticle Composition
Video & Application Note
Dr. Sonja Schneider (Agilent Technologies)
This application note describes the analysis of the lipid components of patisaran using an Agilent 1260 Infinity II Prime Bio LC system and evaporative light scattering detection (ELSD). Read the application to learn about composition and structure of lipid nanoparticles, how to achieve best LC separation for the LNP components, and how to use the ELSD for universal detection.
Learn more about product safety and quality control with HPTLC in Issue 1, 2022 of the CAMAG Bibliography Service (CBS 128)
CAMAG®, in collaboration with Separation Science, is pleased to present Issue 1, 2022 of its CAMAG Bibliography Service (CBS 128). Focusing on product safety and quality control with HPTLC, the latest edition features topics around the analysis of methylglyoxal in Manuka honey, planar solid-phase-extraction (pSPE) screening of chlorinated paraffins as an alternative to time-consuming and complicated GC approaches, quantitative routine analysis of glucose degradation products in sterile solutions, and the development of new HPTLC methods for the detection of falsified drugs. Additionally, we introduce the HPTLC Association Substance Database for natural products, a systematic collection of retention and spectral data of representative substances from different classes, analyzed with different developing solvents and derivatization reagents.
Analysis Methods for Residual Pesticides in Food
Separation Science, in collaboration with Shimadzu, offers a series of application notes demonstrating how the use of LC-MS/MS and GC-MS/MS, often with a modified QuEChERS method, can be used to rapidly quantify residual pesticides in food and beverage samples. This includes a Method for the Determination of 346 Residual Pesticides in Milk, Multiresidue Pesticides Analysis in Curcumin Color Additive Powder, Analysis of Residual Pesticides in Carrot-oleoresin and a Method for the Determination of 313 Residual Pesticides in Black Tea.
Effective Nitrosamines Analysis in Drugs and Solvents
Discover methods for the quantitation of various nitrosamines in drugs and solvents using Shimadzu LC/MS or GC/MS instruments, including: 1) Quantitation of 6 Nitrosamines in 5 Sartans by LC-MS/MS system as per the proposed USP General Chapter <1469>, 2) Quantitation of 6 Nitrosamines in Losartan API by LC-MS/MS system as per the proposed USP General Chapter <1469>, 3) Quantitation of 7 Nitrosamines in API by HSGC-MS/MS as per proposed USP General Chapter <1469> and 4) Quantitation of 8 Nitrosamines in 12 different solvents by LC-MS/MS system.
Solutions for COVID-19 Drug Analysis
Discover methods for the analysis of various therapeutics for the treatment of COVID-19, including: 1) Simultaneous Analysis of Remdesivir and Metabolites in Human Plasma Using Fully Automated Sample Preparation LC/MS/MS System, 2) Simultaneous Analysis of Remdesivir and Metabolites in Human Plasma (LC-MS), 3) Fully Automated Quantification of Meropenem, Tazobactam, Piperacillin and Dexamethasone in Plasma and 4) JP- and EP-Compliant Analysis of Impurities of COVID-19 Drug Dexamethasone.
Simultaneous Analysis of Monoclonal Antibodies using a Novel Dual-channel UHPLC Instrument and Orthogonal Chromatography
Thermo Fisher Scientific
Learn how to perform simple and rapid high-throughput analysis of biotherapeutics using different chromatographic techniques at one time.
Impact of Temperature on the Efficiency of High-Temperature GC Columns
This technical overview examines the effects of temperatures above 400 °C on the integrity of the Agilent J&W DB-5ht column phase and brittleness of the column over extended periods of time.
Identity Confirmation and Accurate Quantitation of a Genotoxic Impurity in an Active Pharmaceutical Ingredient by UHPLC-UV coupled to a Single Quadrupole Mass Detector
Thermo Fisher Scientific
Learn how the combination of UV and a single quadrupole mass detector is a powerful tool for API analysis in early stages of development, enabling confirmation of expected impurities.
Shimadzu 'Excellence in Science' educational portal
The Shimadzu 'Excellence in Science' portal, a collaboration between Separation Science and Shimadzu where you will find links to useful information and resources relevant to analytical scientists working across pharmaceutical, food, environmental and clinical disciplines.
Comprehensive Screen of Acidic/Neutral/Basic Drugs from Blood and Urine Featuring a Direct Elution to Analysis Technique via LC-MS/MS
This application note exploits the highly retentive, universal nature of UCT’s polymeric Styre Screen® HLD sorbent for the analysis of acidic, neutral, and basic compounds in blood and urine. The generalized protocol not only minimizes unnecessary sample preparation steps, but also it allows for the LC-MS friendly elution solvent to go directly into an autosampler vial for further analysis.
Automated Aroma Profiling of Wine using Sorptive Extraction with GC×GC–TOF MS/SCD
This study demonstrates the use of headspace sorptive extraction coupled with GC×GC–TOF MS/SCD to provide comprehensive information on wine aroma and odour taints on a single, fully automated, cryogen-free platform.
Analysis of Semivolatile Organic Compounds in Drinking Water on the Agilent 8890 GC and 5977 GC/MSD with Extended Calibration Range
This application note coupled an Agilent 8890 GC with an Agilent 5977 MSD to analyse semivolatile organic compounds in drinking water sources according to United States Environmental Protection Agency (EPA) method 525. This workflow generated results that met method 525 calibration criteria, and demonstrated that this system is sufficiently sensitive to detect most of the low-level standards analysed.
Doubling Chromatographic Throughput using Dual LC
Thermo Fisher Scientific
Learn how the Thermo Scientific Vanquish Duo system for Dual LC enables two sample runs concurrently, so samples run in half the time, improving pharmaceutical drug analysis.
High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity
In this study, a simple pass-through SPE sample cleanup was developed as an alternative to immunoaffinity columns and applied to lipid-rich CBD oils.
Endrin and DDT Stability Study for Drinking Water Methods with an Agilent 8890 GC/5977B GC/MSD Combined System
Endrin and 4,4'-DDT, organochlorine pesticides, can be used to determine flowpath inertness and cleanliness of a GC system. This application brief demonstrates that the Agilent 8890 GC can meet the instrument performance check criteria established by US EPA Method 525.2 and Method 525.3 as well as other internal standards for drinking water quality.
Application of Saw Tooth Gradients using PSS WinGPC UniChrom
Saw tooth gradients require a significant number of entries in a gradient table. This application note describes how saw tooth gradients can be programmed in PSS WinGPC UniChrom.