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Methods & Applications

Separation Science offers free learning from the experts covering methods & applications for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Featured Methods & Applications

Simultaneous Analysis of Monoclonal Antibodies using a Novel Dual-channel UHPLC Instrument and Orthogonal Chromatography

Application Note

Thermo Fisher Scientific

Learn how to perform simple and rapid high-throughput analysis of biotherapeutics using different chromatographic techniques at one time.

Impact of Temperature on the Efficiency of High-Temperature GC Columns

Application Note

Agilent

This technical overview examines the effects of temperatures above 400 °C on the integrity of the Agilent J&W DB-5ht column phase and brittleness of the column over extended periods of time.

Identity Confirmation and Accurate Quantitation of a Genotoxic Impurity in an Active Pharmaceutical Ingredient by UHPLC-UV coupled to a Single Quadrupole Mass Detector

Application Note

Thermo Fisher Scientific

Learn how the combination of UV and a single quadrupole mass detector is a powerful tool for API analysis in early stages of development, enabling confirmation of expected impurities.

Shimadzu 'Excellence in Science' educational portal

Application Notes

Shimadzu

The Shimadzu 'Excellence in Science' portal, a collaboration between Separation Science and Shimadzu where you will find links to useful information and resources relevant to analytical scientists working across pharmaceutical, food, environmental and clinical disciplines.

Comprehensive Screen of Acidic/Neutral/Basic Drugs from Blood and Urine Featuring a Direct Elution to Analysis Technique via LC-MS/MS

Application Note

UCT

This application note exploits the highly retentive, universal nature of UCT’s polymeric Styre Screen® HLD sorbent for the analysis of acidic, neutral, and basic compounds in blood and urine. The generalized protocol not only minimizes unnecessary sample preparation steps, but also it allows for the LC-MS friendly elution solvent to go directly into an autosampler vial for further analysis.

Automated Aroma Profiling of Wine using Sorptive Extraction with GC×GC–TOF MS/SCD

Application Note

SepSolve Analytical

This study demonstrates the use of headspace sorptive extraction coupled with GC×GC–TOF MS/SCD to provide comprehensive information on wine aroma and odour taints on a single, fully automated, cryogen-free platform.

Analysis of Semivolatile Organic Compounds in Drinking Water on the Agilent 8890 GC and 5977 GC/MSD with Extended Calibration Range

Application Note

Agilent

This application note coupled an Agilent 8890 GC with an Agilent 5977 MSD to analyse semivolatile organic compounds in drinking water sources according to United States Environmental Protection Agency (EPA) method 525. This workflow generated results that met method 525 calibration criteria, and demonstrated that this system is sufficiently sensitive to detect most of the low-level standards analysed.

Doubling Chromatographic Throughput using Dual LC

Application Note

Thermo Fisher Scientific

Learn how the Thermo Scientific Vanquish Duo system for Dual LC enables two sample runs concurrently, so samples run in half the time, improving pharmaceutical drug analysis.

High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity

Application Note

Restek

In this study, a simple pass-through SPE sample cleanup was developed as an alternative to immunoaffinity columns and applied to lipid-rich CBD oils.

Endrin and DDT Stability Study for Drinking Water Methods with an Agilent 8890 GC/5977B GC/MSD Combined System

Application Note

Agilent

Endrin and 4,4'-DDT, organochlorine pesticides, can be used to determine flowpath inertness and cleanliness of a GC system. This application brief demonstrates that the Agilent 8890 GC can meet the instrument performance check criteria established by US EPA Method 525.2 and Method 525.3 as well as other internal standards for drinking water quality.

Application of Saw Tooth Gradients using PSS WinGPC UniChrom

Application Note

PSS

Saw tooth gradients require a significant number of entries in a gradient table. This application note describes how saw tooth gradients can be programmed in PSS WinGPC UniChrom.

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