Temperature and Retention

by | May 9, 2019

This technical article takes a closer look at the influence of temperature on the separation

This technical article takes a closer look at the influence of temperature on the separation.

Ever have an HPLC method that specifies “ambient” as the column temperature? That’s a good sign of potential problems in the future for the method. We’ll get back to the interpretation of “ambient” in a bit, but first, let’s take a look at the influence of temperature on the separation.

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