How can we describe the mechanism at the basis of HILIC retention? We will answer this question and give you an insight into the main interactions that govern chromatographic separation in HILIC mode.
HILIC retention mechanism is more complex in comparison to other chromatographic separation modes and many scientists are still striving to understand it fully. It is often described as a mixed-mode mechanism, where several kinds of interaction contribute simultaneously to the overall retention. In order to understand the HILIC mechanism, it is useful to have a look at the main retention model used to describe the widespread reversed-phase liquid chromatography (RPLC), familiar to most chromatographers.
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