By viewing this presentation, you will:
- Understand how to obtain good resolution by utilizing selectivity
- Learn the basics of different stationary phase particle characteristics
- Discover how to choose different stationary phases based on compound classes
- Find out how the mobile phase can be used to change the selectivity of columns
Event Overview
Are you new to HPLC method development, or maybe you would welcome a quick refresh? This presentation shows you how to select an appropriate column based on the types of molecules you are looking to separate, and then highlights how the choice of mobile phase can be optimized to help maximize selectivity. The focus of this presentation is on the reversed-phase separation of small molecules, considering stationary phase interactions, and the impact of mobile phase pH on retention, selectivity, and peak shape.
What you need to know:
Format: On-demand
Duration: Approximately 1 hour
Presenter:
Genevieve Hodson
Manager, Technical Support Team, Phenomenex
Genevieve graduated from the University of Texas with a BS in Chemistry located in her home town of Austin, Texas. She has become an expert at analytical chemistry from her previous experience performing purity analysis, customer-specific methods, validations, QC testing, and stability studies on a wide range of small molecules, including pharmaceuticals, pesticides, explosives, and illegal substances. She started at Phenomenex as a technical specialist, and now she enjoys continuing to learn about chromatography every day through helping customers with all their chromatography needs.
Sponsor:
