Extra HPLC column effects

by | Mar 27, 2023

This module explores some of the other problems that can not always be attributed to the column.

HPLC_UHPLC_TS_ContentDo you work with HPLC or UHPLC? "HPLC & UHPLC Troubleshooting: A Performance Qualification Approach” is an intensive online course that teaches you the ins and outs of solving problems that occur with your high-performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) methods. You will learn how to qualify the performance of your HPLC or UHPLC using specific tests that also can be used for isolating existing problems. More importantly, you’ll learn how to prevent many of these problems from happening in the first place.

No previous experience with HPLC or UHPLC systems is assumed; however, much of the course will appeal to the more experienced worker.

In the 'Extra-Column Effects' module in the 'Physical Column Problems' section, we'll explore some of the other problems that can not always be attributed to the column.

You will learn about:

  • Other factors beyond the column that cause problems
  • Chromatographic characteristics that are indicative of extra-column band broadening
  • Tailing of early vs. late peaks
  • How to reduce the risk of extra-column band broadening
  • How narrow-bore columns for LC-MS or UHPLC can make things worse
  • Extra-column volume and resolution
  • Tubing conversions
  • The benefit of PEEK (Polyetheretherketone) fittings
  • Compression fittings
  • The effect of the detector cell volume on the appearance of the chromatogram

This module has a complementary quiz. By correctly answering all of the quiz questions for all of the modules in the 'HPLC and UHPLC Troubleshooting: A Performance Qualification Approach' course you will be able to download your certificate of completion.

By taking this online course you will learn about the operating principles of each module in an HPLC and UHPLC system whilst gaining knowledge of basic HPLC theory as it applies to troubleshooting and instrument maintenance. You will understand why performance qualification (PQ) is so important to ensure the reliable operation of your HPLC and UHPLC and improve the quality of your results. Furthermore, you will learn proven techniques for systematic problem-solving and instrument maintenance and the most effective, time-saving, money saving approaches to preventing common hardware problems and method failures.

The course price is US$ 249 for one user for 12 months of access. Multi-user pricing is also available, as are site licences for larger teams with multiple course requirements.

Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique education resource for analytical chemists. Currently we offer HPLC training, LC-MS training, GC training and GC-MS training.

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