Learn why retention (k') is defined the way it is and the reason it has an optimum range of values.

Discover some processes and practices to help prevent problems from happening in the first place.

Learn about the basics and fundamentals of HPLC including instrumentation, measurements and parameters, chemistry, and quantitation.

From the start of the flow patch, the different types of solvent delivery systems used in HPLC, and effective removal of dissolved gas from the solvent.

The typical design of injector valves and a comparison of the three different types of autosamplers that are used to load those valves.

How the characteristics of connecting tubing (inertness, pressure resistance, internal volume) fit the requirements in different parts of the HPLC system.

Learn about measures for selectivity (alpha) and efficiency (N) and how they affect chromatographic results.

Discover how the quality of a separation is measured by resolution. Understand how it’s defined and measured, and why the measurement can be misleading.

Learn about reversed-phase HPLC, the parameters that control the way it works and why it is the most widely used mode of HPLC.