Gradient Distortion Problems in HPLC

by | Mar 27, 2023

Learn how gradient distortion can affect your separation and understand the role of dwell and mixing.

HPLC_UHPLC_TS_ContentDo you work with HPLC or UHPLC? "HPLC & UHPLC Troubleshooting: A Performance Qualification Approach” is an intensive online course that teaches you the ins and outs of solving problems that occur with your high-performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) methods. You will learn how to qualify the performance of your HPLC or UHPLC using specific tests that also can be used for isolating existing problems. More importantly, you’ll learn how to prevent many of these problems from happening in the first place.

No previous experience with HPLC or UHPLC systems is assumed; however, much of the course will appeal to the more experienced worker.

In the 'Performance Qualification: Part 2, On-Line Mixing' section we present simple tests to run that determine the accuracy of the mixing process. When gradient methods are run, differences in system dwell volume are the number one reason why gradient methods are hard to transfer. We show how to measure dwell volume and how to compensate for differences between systems. How do HPLC and UHPLC compare in terms of on-line mixing? You can program the system to generate just about any imaginable gradient shape - how can you tell if the system can keep up with your demands?

This module focuses on 'Gradient Distortion Problems'.

You will learn about:

  • Gradient distortion and how it can affect your separation
  • The role of dwell and mixing
  • HPLC vs. UHPLC step tests
  • Gradient non-linearity
  • Failed GPV tests

This module has a complementary quiz. By correctly answering all of the quiz questions for all of the modules in the 'HPLC and UHPLC Troubleshooting: A Performance Qualification Approach' course you will be able to download your certificate of completion.

By taking this online course you will learn about the operating principles of each module in an HPLC and UHPLC system whilst gaining knowledge of basic HPLC theory as it applies to troubleshooting and instrument maintenance. You will understand why performance qualification (PQ) is so important to ensure the reliable operation of your HPLC and UHPLC and improve the quality of your results. Furthermore, you will learn proven techniques for systematic problem-solving and instrument maintenance and the most effective, time-saving, money saving approaches to preventing common hardware problems and method failures.

The course price is US$ 249 for one user for 12 months of access. Multi-user pricing is also available, as are site licences for larger teams with multiple course requirements.

Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique education resource for analytical chemists. Currently we offer HPLC training, LC-MS training, GC training and GC-MS training.

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