Solutions for Oligonucleotide Drug Analysis

by | Sep 22, 2021

Covering thermal stability analysis of nucleic acid drugs, and the determination of the molecular weight and structure of oligonucleotide therapeutics.

Separation Science, in collaboration with Shimadzu, offers a series of application notes providing solutions for oligonucleotide drug analysis. Click on the button below to download your personal copies.

Oligonucleotide Drug Analysis


Thermal Stability Analysis of Nucleic Acid Drugs by TMSPC™-8 Tm Analysis System
Shimadzu_App1Thermal stability analysis (Tm analysis) of a nucleic acid was conducted using a Shimadzu UV-1900i ultraviolet-visible (UV-Vis) spectrophotometer and TMSPC-8 Tm analysis system. This system provides a simple method to calculate the Tm value by measuring the absorbance transition at 260 nm at increasing temperatures.

Learning outcome:
Discover how the TMSPC-8 Tm analysis system can simplify thermal stability analysis of nucleotides.

Synthesis Confirmation for Nucleic Acid Medicines - Rapid Sequence Confirmation Using a MALDI-TOF Mass Spectrometer
Shimadzu_App2This application shows how the molecular weight and base sequence of a synthetic nucleic acid can be determined using MALDI-TOF mass spectrometry.

Learning Outcome:
See how Shimadzu’s benchtop MALDI-8020 mass spectrometer makes it easy to confirm the molecular weight and base sequence of nucleic acids.

Verification of Quantitativity and Confirmation of Molecular Weight of Oligonucleotide Therapeutics Using LCMS™-8060
Shimadzu_App3Here we introduce the analysis of a 20 base 2’-MOE modified oligonucleotide using an LCMS-8060 triple quadrupole mass spectrometer. A calibration curve for 1 to 300 ng/mL was obtained, and the molecular weight was confirmed within a small error range by deconvolution function from the scan data.

Learning Outcome:
Learn how Shimadzu’s LCMS-8060 can be used to determine the molecular weight of synthetic oligonucleotides.

Determination of Molecular Mass and Quantification of Oligonucleotide Therapeutics Using Quadrupole Time-of-Flight Mass Spectrometer LCMS™-9030
Shimadzu_App4This application shows an example analysis of a 20 base 2’-MOE modified oligonucleotide using the LCMS-9030 Q-TOF mass spectrometer. The molecular mass was determined with an error of just 0.05 ppm. A calibration curve was prepared using the MRM mode, demonstrating linearity within a range of 1–1000 ng/mL.

Learning outcome:
Learn how the accurate mass measurement of Shimadzu’s LCMS-9030 can be used to identify the structure of oligonucleotide therapeutics.

Don't miss out on the latest COVID-19, nitrosamine analysis or LC/MS bioanalysis of antibody drugs applications. Click below for PDFs.

Click here to access the COVID-19 app notes >>

Click here to access the nitrosamine analysis app notes >>

Click here to access the LC/MS bioanalysis of antibody drug app notes >>

Related Content