In gas chromatography 80% of the trouble is caused by the injection system. Also here we have seen that by degradation of sample components, O-rings, back flash and septa, extra peaks can be generated. Besides this, the conditions used for injecting the sample can also produce serious extra peaks.
Animal feed occasionally has some degree of contamination by Aspergillus spp. Even pasteurized milk at times contains aflatoxin M1 (AFM1). Recently the incidence of aflatoxins in animal feed ingredients has increased thus increaasing the possibility of the occurance of Aflatoxin M1 in milk. However, Aflatoxin M1 is a toxic (100% as Aflatoxin B1), carcinogenic (33% as Aflatoxin B1) and mutagenic (3.3 % of Aflatoxin B1) metabolite compound. This article is a general review of Aflatoxin M1, its characteristics, its methods of analysis and performance characteristics of different methods of analysis for aflatoxin M1 in milk.