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Welcome to Separation Science


Separation Science is the leading global portal for fundamentals, best practice and troubleshooting information for users of chromatographic and mass spectrometric techniques. Featuring articles, applications, webinars, workshops and conferences, Separation Science is the industry's 'go to' information resource.



HILIC Solutions #8: Column Equilibration in HILIC

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention factors. In this way it is possible to narrow the peak width of the later eluted compounds and shorten the run time. In this article, the importance of column equilibration is considered for optimum efficiency.


Changing from Helium to Nitrogen and Maintaining the Separation Efficiency in the Same Analysis Time

For several reasons, there is interest in replacing helium with a different carrier gas in gas chromatography. Hydrogen is the obvious choice but there is a concern on safety and reactivity. It is also possible to use nitrogen but this is often not considered because it has a low optimal flow and velocity.





1st Advances in Forensics & Toxicology eSeminar

Sponsors:  AB SCIEX, Bruker, Gerstel

Separation Science, in collaboration with sponsors AB SCIEX, Bruker and Gerstel has developed an online eSeminar comprising a scientific programme covering key topics of analytical importance to the forensics/toxicology industries. This unique on-demand event brings together some of the leading exponents in forensics/toxicology analysis and provides an interactive learning environment for scientists working in these fields.

2nd Advances in Food Safety Analysis eSeminar

Sponsors:  AB SCIEX, Merck Millipore, Shimadzu, Supelco/Sigma-Aldrich, Thermo Fisher Scientific, Waters

Separation Science and scientific partner LGC has developed an online eSeminar focusing on new methods and applications in food safety analysis. This unique event brings together some of the leading exponents in food safety analysis and provide an interactive learning environment for scientists working in these fields.

1st Advances in Enviro Analysis eSeminar

Sponsors:  Bruker, PerkinElmer, Supelco/Sigma Aldrich and Waters

Separation Science has developed an online eSeminar focusing on new methods and applications in environmental analysis. This unique on-demand event brings together some of the leading exponents in environmental analysis and provide an interactive learning environment for scientists working in these fields.

1st Quality by Design for Pharmaceutical Analytical Methods eSeminar

Scientific Partner: Agilent Technologies

This seminar series, produced by Separation Science in collaboration with Agilent Technologies, features industry experts highlighting some of the key drivers for QbD as well as the ways QbD concepts can be applied to help ensure methods are rugged and robust.

1st Advances in Food Safety eSeminar 

Scientific Partner: LGC
Sponsors: Bruker, Gerstel, Supelco/Sigma-Aldrich, Waters

You will be provided with a good overview of chromatographic and mass spectrometric methods for the analysis of environmental and processing contaminants in food matrices, together with updates on new methodologies for monitoring and maintaining food quality and authenticity. Experts in these technologies will provide details on sample preparation strategies, liquid and gas chromatographic separation protocols and mass spectrometric detection, as well as highlighting current trends and emerging issues in this important analytical field.


Self-Paced Training


HPLC Basics, Equipment, and Troubleshooting

What does it cover?

“HPLC Basics, Equipment, and Troubleshooting” explains chromatography in practical terms from the ground up.
Here’s what you’ll learn in this online course:
• The basic principles of HPLC
• The five fundamental HPLC modes
• The easy and logical way to adjust experimental variables to get a good separation
• The best approach to use for various chromatographic applications
• How to maximize column life
• Special techniques such as gradient elution, quantitation, and sample pretreatment
• The operating principles of each module in an HPLC system
• Proven techniques for systematic problem-solving and instrument maintenance
• The most effective, timesaving, money saving approaches to preventing common hardware problems and method failures.

What will I get from this is course?

You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient. What you learn will demystify your instrument. You’ll find that all of the perplexing and frustrating problems your experience have simple and logical solutions. And, better yet, you’ll learn that most of these problems are preventable. The techniques you learn from this course will make you more effective at work and save you big headaches! If you’ve never taken a formal course or if you need a refresher, it’s time to learn HPLC the right way. Students tell us that this “extremely practical course” is a must for everyone who uses HPLC.

Advanced HPLC Method Development Using Quality by Design

What does it cover?

The central tenant of Quality by Design (ICH Q8) is that quality cannot be tested into a product – instead it must be designed into the product. When the product is an HPLC method, QbD strategies can guide the development process to result in a standardized method development prcedure, more easily validated methods, and methods that are easier to use and adjust in routine applications.
 “Advanced HPLC Method Development Using Quality by Design” is an intensive online class that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods.

Introduction to Gas Chromatography

What does it cover?

The ‘Introduction to Gas Chromatography’ On-Line Tutorial is basic introduction to the theory and practice of gas chromatographic analysis. It is designed to bring GC users to a more comfortable and capable level of proficiency in instrument operation, optimization, and troubleshooting. Lessons are presented in a visually engaging and accessible manner through narrated on-line video presentations. In full recognition of the difficulty in setting large blocks of uninterrupted time for training; the lessons are presented in short segments that can be easily fit into even the busiest schedules.
• This course presents the basic processes and instrumentation involved with GC analysis.
• The level of material is specifically designed for GC users without extensive formal training.
• Applicable theory is covered to the extent that it helps guide best practices, method optimization and effective troubleshooting.

What will I get from this course?

Upon successful completion of the tutorial, trainees will have increased confidence and skills in using gas chromatographs. They will better understand how GC works, be more competent at detecting and diagnosing problems, and will be better at interpreting the quality of analytical results. In the end, trainees will be more capable and comfortable using gas chromatographs.

Intermediate Gas Chromatography

What does it cover?

The Intermediate Gas Chromatography tutorial is designed for GC users who have a fundamental understanding of how gas chromatography works and who are ready to develop a more in-depth understanding of the technique. As with the ‘Introduction to Gas Chromatography’ course, the ‘Intermediate Gas Chromatography’ course is organized into sections and modules. The sections focus on a given theme and the modular structure underneath exposes students to the information within time-effective sections. The ‘Intermediate Gas Chromatography’ course is a logical next step after completing the ‘Introduction to Gas Chromatography’ course.

What will I get from this is course?

Upon successful completion of the course, trainees will have increased knowledge and skills in development of gas chromatography methods. They will gain an understanding of both qualitative and quantitative methods, as well as expanding their skills to encompass areas such as fast GC, and further developing maintenance and troubleshooting abilities.

LC-MS/MS for Chromatographers

What does it cover?

With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector. You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

What will I get from this course? 

You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise. You will receive a printed copy of the approximately 515 slides included in this course to supplement your note-taking as you view the class. 





Mass Spectrometry On-demand

Sponsors:  AB SCIEX, Agilent, Bruker, JEOL,, Peak Scientific, Scientific Resources, Shimadzu, Supelco, Waters

Mass Spectrometry is a world-class international conference covering fundamentals, best practice and applications in mass spectrometry and mass spectrometry coupled techniques. The 2013 conference took place in Singapore, offering delegates the chance to learn from some of the world's top experts in the use of mass spectrometry and related techniques. The conference is now available as an on-demand online event providing access to this knowledge base to a wider global audience. On this site you'll find links to presentations given in Singapore which you can listen to on-demand from your own laboratory or office. Learn about the importance of the sample molecule when determining the correct method; discover how to successfully hyphenate chromatographic systems; and what are the pros and cons of the various MS interfaces? Discover how to interpret and understand the data you generate, and understand how MS can be applied to various analytical challenges across market sectors and industries.



Applying Best in Class Size Exclusion and Hydrophilic Interaction Chromatography Technologies to the Analysis of Glycoproteins

Date: Thursday 16 April, 2015
Time: 8 am PDT / 11 am EDT / 4 pm GMT / 5 pm CET

Presenters: Brian Gau and Kevin Ray (both Sigma-Aldrich)

The first part of this webinar will introduce and elaborate on a fast size exclusion chromatography methodology for the high-­throughput analysis of antibodies and related biotherapeutic proteins. The second part of this webinar will present a released glycan profiling methodology that utilizes HILIC­-FLD-­MS to deliver sensitive quantification and structural identification of glycans.

Multiple Fast and Easy Possibilities for Disinfection Control 

Date: 23 April, 2015
Time: 9 am EST, 2 pm BST, 3 pm CEST

Presenter: Carolin Klein (Merck KgaA)

The presentation starts by outlining the advantages and disadvantages of different disinfectants such as chlorine, ozone, chlorine dioxide and cyanuric acid. It looks at which disinfectant is used where and why and which one is more efficient and why. After this overview the different possibilities for monitoring disinfection residues in water are presented. Attendees to the presentation will learn about fast and easy control with a test strip but also discover an easy photometric measurement of disinfection parameters with the new mobile colorimeter - Spectroquant® Move Cl2/O3/ClO2/CyA/pH.


Jet Stream Proteomics – Enabling Standard Flow Chromatography for Robust Discovery Proteomics

Presenters: Christopher Petzold (Lawrence Berkeley Lab, California, USA) and Christine Miller (Agilent Technologies, USA)

This webinar will discuss the details of Jet Stream proteomics for discovery workflows and how the robust nature of this approach impacts sample analysis.

A Day in the Life of a Food Contract Lab – Routine Metals Analysis in Food 

Presenter: Carlos J. Sepúlveda Ibarra (Agrolab México)

In this webinar the typical steps to obtaining precise and accurate quantitative metals analysis in a variety of food matrices by ICP-MS are explained. Total metals analysis including sample preparation, instrumental analysis and QA/QC reporting as well as final results will be discussed.

The Analysis of Pharmaceuticals in Groundwater, Surface Water, and Drinking-Water Supplies by Liquid Chromatography/Triple Quadrupole Mass Spectrometry 

Presenter: Imma Ferrer (Center for Environmental Mass Spectrometry, University of Colorado, Boulder, USA)

This webinar explains how a combination of solid-phase extraction and liquid chromatography/triple quadrupole mass spectrometry can be used to identify 20 commonly prescribed pharmaceuticals and 8 hormones that may occur in source waters for drinking water supplies.


Smelling Shelf Life – Profiling Volatile Organic Compounds

Presenters: Carston Muller (Markes International) 

The webinar presents the latest findings of VOC profiling of fresh produce as part of the EU project QUAFETY. It specifically will cover issues of VOC sampling of perishable samples, subsequent analysis on TD-GC-MS-TOF system and following data processing and evaluation of VOC profiles.

Software Solutions Enabling Open Access Workflows

Presenters: Michael Jones (Waters Corporation) and Dr Mark Bayliss (Virscidian, Inc.)

This webinar will discuss Waters Openlynx Open Access Solutions and new workflows designed to streamline the implementation of supercritical fluid chromatography (SFC) technologies focused on supporting Medicinal Chemistry and Synthetic Chemistry laboratories.

Efficient High Through-put UHPLC-HRMS Based Screening and Quantification of Veterinary Drugs

Presenter: Anton Kaufmann (Official Food Control Authority, Canton of Zurich, Switzerland)

The use of veterinary drugs is an integral part of the process of raising food producing animals. However, it is important to monitor residues from some of these drugs as they may cause detrimental health effects to humans.


HPLC Solutions #130: Resources #1: Teach a Person to Fish

I’m sure you’ve all heard the proverb that says if you give a person a fish, you’ll feed them for a day, but if you teach a person to fish, you’ll enable them to feed themselves. There are lots of on-line options for “fishing lessons” through LC Resources and Separation Science, many with free access. In the next few HPLC Solutions instalments, we’ll look at other resources focused on specific tasks you need to undertake.



MS Solutions #28: Quality: The Third Side of the Analytical Success Triangle

As analytical chemists, we spend considerable time thinking about and working with basic scientific principles combined with the technology and products that allow us to solve our analytical problems. However, it is essential for success of any method and for the long-term success of a laboratory operation that our science and technology are supported by a quality system that ensures continuing achievement of our operational goals. We therefore present a triangle concept for analytical success: One side of the triangle shows our need to understand fundamental scientific concepts. A second, and equally sized side, is the ability to apply current technology, applications, software, and techniques in the laboratory. Finally, the base of this equilateral triangle is the quality system. If any one side of the triangle is given inadequate attention or, worse yet, is missing then we cannot hope to achieve overall success for the laboratory operation.



Learn more about CORTECS solid-core particle columns, which offer particle sizes ideal for HPLC, UHPLC, and UPLC platforms. These columns are developed for a simple, seamless method transfer with consistent results across different particle sizes and column configurations.


Separation Science is pleased to announce the Chromolith® Technology Microsite. Click on the button below to find details of the educational content available on this site, including technology articles, applications, videos and online resources.


Don't miss out on regular learning materials for chromatography, mass spectrometry and related techniques from Separation Science.


Separation Science, in collaboration with Phenomenex, has developed a microsite dedicated to issues surrounding the use of core-shell particle columns. In this section, you'll find articles describing some of the technology behind these columns, applications in small and large molecule separations, as well as a selection of videos and tutorials.


Presentations from Mass Spectrometry 2013 are now available online, providing analysts globally with access to talks given by many of the world's leading authorities on the use and application of MS techniques. For the one-off price of US$79 you can gain access to presentations describing the importance of the sample molecule when determining the correct method; how to successfully hyphenate chromatographic systems;  and the pros and cons of the various MS interfaces? Discover how to interpret and understand the data you generate, and understand how MS can be applied to various analytical challenges across market sectors and industries.

Access is for 6 months and each presentation can be viewed multiple times. For full details on this online resource and how to access it click the 'Read more' button below.