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Welcome to Separation Science

 

Separation Science is the leading global portal for fundamentals, best practice and troubleshooting information for users of chromatographic and mass spectrometric techniques. Featuring articles, applications, webinars, workshops and conferences, Separation Science is the industry's 'go to' information resource.

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Structural Motifs in Primary Oxidation Products of Palmitoyl-arachidonoyl-phosphatidylcholines by LC-MS/MS

Ana Reis from the Mass Spectrometry Center, UI-QOPNA, Chemistry Department, University of Aveiro, Aveiro, Portugal answers questions on her research into structural motifs in primary oxidation products of palmitoyl-arachidonoyl-phosphatidylcholines by LC-MS/MS.

 

Development of a Rapid Liquid Chromatography Tandem Mass Spectrometry Method for Determination of Trace Caffeine in Surface Water

A rapid LC/MS/MS method for the quantification of caffeine in surface water samples is presented. This method employs large volume injection on a single LC reversed-phase column with detection in ESI positive mode. The method is robust and has the advantage of requiring no extensive sample preparation steps. 

 

 
 
 

UPCOMING


Don’t leave it to chance...are you selecting the correct consumables for your GC & GC-MS system?

Presenter: Ruth Jones (Thermo Fisher Scientific, UK)
Date: 2 October, 2014
Time: 8am PDT / 11am EDT / 4pm UK / 5pm CET

Selection of the correct consumables for your GC & GC-MS system is critical in order to ensure that optimum performance is achieved. In many cases, this does not happen, leading to poor chromatography, non-reproducible results and more frequent instrument downtime

The Many Facets of GC Troubleshooting

Presenter: Kathy Stenerson (Supelco, USA)
Date: Tuesday 7 October, 2014
Time: 8am PDT / 11am EDT / 4pm BST / 5pm CET

Time is money in a testing laboratory, which means that instrument down time can translate into lost revenue. This seminar will present some tips and tricks to help you decrease instrument downtime and increase productivity by streamlining your troubleshooting and method analysis processes. We will discuss issues which can affect the quality of your chromatographic analysis such as selection of gas purifiers, injection technique, and liner type. Common problems in GC analysis will also be covered; including issues such as peak shape, adsorption, contamination, bleed, etc., as well as indicators of when to replace consumable items such as liners and seals.

Application of LC-QTOF in the Routine Screening Analysis of Veterinary Drugs and Evaluation of LC-QTOF Data

Presenter:Wolfgang Radeck (Federal Office of Consumer Protection and Food Safety, Berlin, Germany)
Date: 15 October, 2014
Broadcast #1: 7 am EDT / 12 pm BST / 1 pm CET
Broadcast #2: 12 pm EDT / 5 pm BST / 6 pm CET

This webinar presents a critical view of the possibilities of LC-QTOF applications in the routine screening of veterinary drugs in matrices from animals for human consumption. It covers the classification of screening methods, legislation, instrumentation and data evaluation.

ON-DEMAND


An Introduction to Chromatographic ICP-MS analysis and Its Applications to The Analysis of Environmental Waters

Presenter: Armando Durazo (The University of Arizona, Tucson, Arizona, USA)

An introduction into chromatographic ICP-MS (including ICP-MS source, ionization, etc.), GC-ICPMS for halogenated disinfection byproducts (DBPs) in water extracts, IC-ICPMS for halides and oxyhalides and also iodinated contrast media (ICMs).

Tips and Tricks – Getting the Best out of your GC System

Presenter: Ruth Jones (Thermo Fisher Scientific)

This webinar covers method development in GC, common problems encountered with GC analysis and suggested tips to resolve these problems. Optimisation tips to lower your running costs whilst maintaining efficiency and output.

 
 

eSeminars

UPCOMING


Advances in Food Safety eSeminar 2014

Dates: 16, 18, 23 & 25 September, 2014
Times:
Broadcast #1: 09.00 UK / 10.00 CET / 13.30 New Delhi / 15.00 Bangkok / 16.00 Beijing & Singapore / 17.00 Tokyo & Seoul
Broadcast #2: 08.00 PDT / 11.00 EDT / 16.00 UK / 17.00 CET

Separation Science, in collaboration with sponsors AB SCIEX, Merck Millipore, Thermo Fisher Scientific, Shimadzu, Supelco/Sigma-Aldrich and Waters, and scientific partner LGC has developed an online eSeminar focusing on new methods and applications in food safety analysis. Taking place across 16, 18, 23 and 25 September, this unique event will bring together some of the leading exponents in food safety analysis and provide an interactive learning environment for scientists working in these fields.

ON-DEMAND


Advances in Enviro Analysis

Sponsors:  Bruker, PerkinElmer, Supelco/Sigma Aldrich and Waters

Separation Science has developed an online eSeminar focusing on new methods and applications in environmental analysis. This unique on-demand event brings together some of the leading exponents in environmental analysis and provide an interactive learning environment for scientists working in these fields.

Quality by Design for Pharmaceutical Analytical Methods eSeminar

Scientific Partner: Agilent Technologies

This seminar series, produced by Separation Science in collaboration with Agilent Technologies, features industry experts highlighting some of the key drivers for QbD as well as the ways QbD concepts can be applied to help ensure methods are rugged and robust.

Advances in Food Safety eSeminar 2013

Scientific Partner: LGC
Sponsors: Bruker, Gerstel, Supelco/Sigma-Aldrich, Waters

You will be provided with a good overview of chromatographic and mass spectrometric methods for the analysis of environmental and processing contaminants in food matrices, together with updates on new methodologies for monitoring and maintaining food quality and authenticity. Experts in these technologies will provide details on sample preparation strategies, liquid and gas chromatographic separation protocols and mass spectrometric detection, as well as highlighting current trends and emerging issues in this important analytical field.

 
 

Self-Paced Learning

AVAILABLE COURSES

HPLC Basics, Equipment, and Troubleshooting

What does it cover?

“HPLC Basics, Equipment, and Troubleshooting” explains chromatography in practical terms from the ground up.
Here’s what you’ll learn in this online course:
• The basic principles of HPLC
• The five fundamental HPLC modes
• The easy and logical way to adjust experimental variables to get a good separation
• The best approach to use for various chromatographic applications
• How to maximize column life
• Special techniques such as gradient elution, quantitation, and sample pretreatment
• The operating principles of each module in an HPLC system
• Proven techniques for systematic problem-solving and instrument maintenance
• The most effective, timesaving, money saving approaches to preventing common hardware problems and method failures.

What will I get from this is course?

You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient. What you learn will demystify your instrument. You’ll find that all of the perplexing and frustrating problems your experience have simple and logical solutions. And, better yet, you’ll learn that most of these problems are preventable. The techniques you learn from this course will make you more effective at work and save you big headaches! If you’ve never taken a formal course or if you need a refresher, it’s time to learn HPLC the right way. Students tell us that this “extremely practical course” is a must for everyone who uses HPLC.

Advanced HPLC Method Development Using Quality by Design

What does it cover?


The central tenant of Quality by Design (ICH Q8) is that quality cannot be tested into a product – instead it must be designed into the product. When the product is an HPLC method, QbD strategies can guide the development process to result in a standardized method development prcedure, more easily validated methods, and methods that are easier to use and adjust in routine applications.
 “Advanced HPLC Method Development Using Quality by Design” is an intensive online class that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods.

Introduction to Gas Chromatography

What does it cover?

The ‘Introduction to Gas Chromatography’ On-Line Tutorial is basic introduction to the theory and practice of gas chromatographic analysis. It is designed to bring GC users to a more comfortable and capable level of proficiency in instrument operation, optimization, and troubleshooting. Lessons are presented in a visually engaging and accessible manner through narrated on-line video presentations. In full recognition of the difficulty in setting large blocks of uninterrupted time for training; the lessons are presented in short segments that can be easily fit into even the busiest schedules.
• This course presents the basic processes and instrumentation involved with GC analysis.
• The level of material is specifically designed for GC users without extensive formal training.
• Applicable theory is covered to the extent that it helps guide best practices, method optimization and effective troubleshooting.

What will I get from this course?

Upon successful completion of the tutorial, trainees will have increased confidence and skills in using gas chromatographs. They will better understand how GC works, be more competent at detecting and diagnosing problems, and will be better at interpreting the quality of analytical results. In the end, trainees will be more capable and comfortable using gas chromatographs.

Intermediate Gas Chromatography

What does it cover?

The Intermediate Gas Chromatography tutorial is designed for GC users who have a fundamental understanding of how gas chromatography works and who are ready to develop a more in-depth understanding of the technique. As with the ‘Introduction to Gas Chromatography’ course, the ‘Intermediate Gas Chromatography’ course is organized into sections and modules. The sections focus on a given theme and the modular structure underneath exposes students to the information within time-effective sections. The ‘Intermediate Gas Chromatography’ course is a logical next step after completing the ‘Introduction to Gas Chromatography’ course.

What will I get from this is course?

Upon successful completion of the course, trainees will have increased knowledge and skills in development of gas chromatography methods. They will gain an understanding of both qualitative and quantitative methods, as well as expanding their skills to encompass areas such as fast GC, and further developing maintenance and troubleshooting abilities.

LC-MS/MS for Chromatographers

What does it cover?

With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector. You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

What will I get from this course? 

You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise. You will receive a printed copy of the approximately 515 slides included in this course to supplement your note-taking as you view the class. 

 
 

HPLC Solutions #122: How to Measure Noise. Part 1

This installment of HPLC Solutions begins a series on different measurements that we make on chromatograms. Measuring the baseline noise generated by an HPLC system may seem like a singularly boring subject. But if you think about it, noise can be a critical factor in the results of our analytical work. It is directly related to the limit of detection and limit of quantification, it can be used to determine the health of the detector, noise is a great diagnostic tool for problems, and can be a limiting factor in the precision of a method.

 

 
 
 

MS Solutions #28: Quality: The Third Side of the Analytical Success Triangle

As analytical chemists, we spend considerable time thinking about and working with basic scientific principles combined with the technology and products that allow us to solve our analytical problems. However, it is essential for success of any method and for the long-term success of a laboratory operation that our science and technology are supported by a quality system that ensures continuing achievement of our operational goals. We therefore present a triangle concept for analytical success: One side of the triangle shows our need to understand fundamental scientific concepts. A second, and equally sized side, is the ability to apply current technology, applications, software, and techniques in the laboratory. Finally, the base of this equilateral triangle is the quality system. If any one side of the triangle is given inadequate attention or, worse yet, is missing then we cannot hope to achieve overall success for the laboratory operation.

 

 
 
 

Separation Science is pleased to announce the Chromolith® Technology Microsite. Click on the button below to find details of the educational content available on this site, including technology articles, applications, videos and online resources.

 
 

19-20 November - Biopolis, Singapore
Two-day price: US$238

This conference will offer an unparalleled series of learning sessions for regular users of chromatography and mass spectrometry techniques. Led by technology experts, delegates will receive an in-depth educational experience covering the fundamentals and best practice of liquid chromatography, gas chromatography, mass spectrometry and associated sample preparation techniques.

 
 

Don't miss out on regular learning materials for chromatography, mass spectrometry and related techniques from Separation Science.

 
 
 
 

Separation Science, in collaboration with LGC, has developed an online eSeminar - Advances in Food Safety Analysis. This free-access eSeminar is now available on-demand and brings together some of the leading exponents in food safety analysis and provide an interactive learning environment for scientists working in these fields.

sponsored by:

 
 

Separation Science, in collaboration with Phenomenex, has developed a microsite dedicated to issues surrounding the use of core-shell particle columns. In this section, you'll find articles describing some of the technology behind these columns, applications in small and large molecule separations, as well as a selection of videos and tutorials.

 
 

Presentations from Mass Spectrometry 2013 are now available online, providing analysts globally with access to talks given by many of the world's leading authorities on the use and application of MS techniques. For the one-off price of US$79 you can gain access to presentations describing the importance of the sample molecule when determining the correct method; how to successfully hyphenate chromatographic systems;  and the pros and cons of the various MS interfaces? Discover how to interpret and understand the data you generate, and understand how MS can be applied to various analytical challenges across market sectors and industries.

Access is for 6 months and each presentation can be viewed multiple times. For full details on this online resource and how to access it click the 'Read more' button below.

 
 
 
 
 

      

Eclipse Business Media Ltd, Regd in England, No. 06513189, Frederick House, Princes Court, Beam Heath Way, Nantwich, Cheshire CW5 6PQ, United Kingdom, T +44 1270 628551, dean.graimes@sepscience.com