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Welcome to Separation Science


Separation Science is the leading global portal for fundamentals, best practice and troubleshooting information for users of chromatographic and mass spectrometric techniques. Featuring articles, applications, webinars, workshops and conferences, Separation Science is the industry's 'go to' information resource.





Secondary Standards as Certified Reference Materials

Presenter: Nicolas Hauser (Sigma-Aldrich)
Sponsor:  Sigma-Aldrich
Date: 27 August, 2015
Time: 8 am PDT / 11 am EDT / 4 pm BST / 5 pm CEST

A Secondary Standard is a reference material that is traceable to, and qualified against, a primary standard, usually obtained from a National or International Metrological Institute or recognized authority such as the U.S. Pharmacopeial Convention or the European Pharmacopoeia . This webinar will provide a detailed overview and understanding of the Quality System, Manufacturing, and Analytical Certification of Secondary Standards.

Spice Up Your Life with MS: Workflows for High Throughput Pesticide Analysis by MS/MS in Challenging Matrices such as Spices and Tobacco

Presenter: Dr Kaushik Banerjee, National Research Centre for Grapes (NRCG), Indian Council of Agricultural Research (ICAR), Pune, India
Sponsor:  Shimadzu
Date: 16 September, 2015
Time: 8 am BST / 9 am CEST / 1:30 pm IST / 3 pm SGT / 5 pm AEST

This exclusive Shimadzu Food Safety Webinar will provide an in-depth know-how about the various mass spectrometry (MS/MS) workflows that are needed to conduct high throughput pesticide analysis in challenging matrices. You will learn about the latest state-of-the-art technologies that mass spec leaders are using globally to deal with pesticide analysis in difficult matrices such as spices and tobacco.

Transfer of USP Methods from Particulate to Monolithic Columns

Presenter: Dr Patrik Appelblad (Merck KGaA, Darmstadt, Germany)
Sponsor:  Merck KGaA
Date: 22 September, 2015
Time: 6 am PDT / 9 am EDT / 2 pm BST / 3 pm CEST

This webinar will focus on regulated chromatographic analysis and how methods can be transferred from one type of column to another with the same chemistry but with different backbones and physical properties. USP monograph methods, originally developed and validated for use with large particle HPLC columns, have been transferred and partially revalidated for use with monolithic columns. The examples in this webinar will be used to emphasise the new possibilities in changing HPLC methods per chapter 621 in United States Pharmacopeia .


Elemental Analysis in Infant Formula, Nutritional Products, and Raw Ingredients by ICP-MS

Presenter: Lawrence Pacquette (Abbott Nutrition, Columbus, Ohio, USA)
Sponsor: Agilent Technologies

In this webinar, Dr Lawrence Pacquette will present how Agilent ICP-MS instruments (7500cx, 7700x and 7900) have not only provided the required sensitivity for ultra-trace elemental analysis, but have also improved the efficiency of Abbott Nutrition’s laboratories for routine testing and helped in producing AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) recognized analytical methods for elemental analysis.

Thermal Desorption-GC; Making VOC Measurements on Land, Sea and in The Air 

Presenter: Alastair Lewis (University of York, UK)
Sponsor: Agilent Technologies

The presentation will include a comparison of current commercial instrument analytical performance against the highly challenging international standards for VOC measurement that are emerging, and the potential breakthroughs possible with high mass accuracy MS and ‘big data’ tools in the future.

Method Development for Pesticide Screening in Food using a GC/Q-TOF with an Exact Mass Pesticide Database

Presenter: Dr Klaus Wilmers (CVUA-MEL, Germany)
Sponsor: Agilent Technologies

In this webinar Dr Klaus Wilmers (CVUA-MEL) will highlight the development of a pesticide screening method using a high resolution TOF system for routine GC residue applications in a food control lab. Starting with the development and the basic approach to use the GC/Q-TOF for the screening of targeted pesticides following the SANCO guidelines, an outlook is given to use the similar approach to search for unknowns.




Advances in Analytical Methods for Biotherapeutics eSeminar

Broadcast Date:  17 September, 2015

Times:  08.00 PDT / 11.00 EDT / 16.00 BST / 17.00 CET

Sponsors:   PSS

Separation Science, in collaboration with PSS, has developed an online eSeminar focusing on analytical and bioanalytical methods for the analysis of biotherapeutic proteins. This one-day event will provide bioanalytical scientists with updates on the latest issues faced by individuals working in this complex field. Taking place in one 2-hr session, on 17 September, this unique event will bring together some of the leading technology experts and provide an interactive learning environment and online resource.

1st Applications and New Developments in ICP-MS eSeminar

Broadcast Dates: 10 & 15 September, 2015

Times: Session 1 - Thursday, 10 September
08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Session 2 - Tuesday, 15 September
08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

 Sponsors:   Thermo Fisher Scientific, PerkinElmer

Spectroscopy Solutions, in collaboration with Thermo Fisher Scientific and PerkinElmer has developed an online eSeminar focusing on applications and new developments in ICP-MS. Taking place in four 2 hr sessions, one each on 10 &15 September, this unique event will bring together some of the leading exponents in ICP-MS and provide an interactive learning environment for scientists working in these fields.


1st Advances in Forensics & Toxicology eSeminar

Sponsors:   AB SCIEX, Bruker, Gerstel

Separation Science, in collaboration with sponsors AB SCIEX, Bruker and Gerstel has developed an online eSeminar comprising a scientific programme covering key topics of analytical importance to the forensics/toxicology industries. This unique on-demand event brings together some of the leading exponents in forensics/toxicology analysis and provides an interactive learning environment for scientists working in these fields.

2nd Advances in Food Safety Analysis eSeminar

Sponsors:   AB SCIEX, Merck Millipore, Shimadzu, Supelco/Sigma-Aldrich, Thermo Fisher Scientific, Waters

Separation Science and scientific partner LGC has developed an online eSeminar focusing on new methods and applications in food safety analysis. This unique event brings together some of the leading exponents in food safety analysis and provide an interactive learning environment for scientists working in these fields.

1st Advances in Enviro Analysis eSeminar

Sponsors:   Bruker, PerkinElmer, Supelco/Sigma Aldrich and Waters

Separation Science has developed an online eSeminar focusing on new methods and applications in environmental analysis. This unique on-demand event brings together some of the leading exponents in environmental analysis and provide an interactive learning environment for scientists working in these fields.

1st Quality by Design for Pharmaceutical Analytical Methods eSeminar

Scientific Partner:  Agilent Technologies

This seminar series, produced by Separation Science in collaboration with Agilent Technologies, features industry experts highlighting some of the key drivers for QbD as well as the ways QbD concepts can be applied to help ensure methods are rugged and robust.

1st Advances in Food Safety eSeminar 

Scientific Partner: LGC
Sponsors: Bruker, Gerstel, Supelco/Sigma-Aldrich, Waters

You will be provided with a good overview of chromatographic and mass spectrometric methods for the analysis of environmental and processing contaminants in food matrices, together with updates on new methodologies for monitoring and maintaining food quality and authenticity. Experts in these technologies will provide details on sample preparation strategies, liquid and gas chromatographic separation protocols and mass spectrometric detection, as well as highlighting current trends and emerging issues in this important analytical field.

Self-Paced Training


HPLC Basics, Equipment, and Troubleshooting

What does it cover?

“HPLC Basics, Equipment, and Troubleshooting” explains chromatography in practical terms from the ground up.
Here’s what you’ll learn in this online course:
• The basic principles of HPLC
• The five fundamental HPLC modes
• The easy and logical way to adjust experimental variables to get a good separation
• The best approach to use for various chromatographic applications
• How to maximize column life
• Special techniques such as gradient elution, quantitation, and sample pretreatment
• The operating principles of each module in an HPLC system
• Proven techniques for systematic problem-solving and instrument maintenance
• The most effective, timesaving, money saving approaches to preventing common hardware problems and method failures.

What will I get from this is course?

You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient. What you learn will demystify your instrument. You’ll find that all of the perplexing and frustrating problems your experience have simple and logical solutions. And, better yet, you’ll learn that most of these problems are preventable. The techniques you learn from this course will make you more effective at work and save you big headaches! If you’ve never taken a formal course or if you need a refresher, it’s time to learn HPLC the right way. Students tell us that this “extremely practical course” is a must for everyone who uses HPLC.

Advanced HPLC Method Development Using Quality by Design

What does it cover?

The central tenant of Quality by Design (ICH Q8) is that quality cannot be tested into a product – instead it must be designed into the product. When the product is an HPLC method, QbD strategies can guide the development process to result in a standardized method development prcedure, more easily validated methods, and methods that are easier to use and adjust in routine applications.
 “Advanced HPLC Method Development Using Quality by Design” is an intensive online class that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods.

Introduction to Gas Chromatography

What does it cover?

The ‘Introduction to Gas Chromatography’ On-Line Tutorial is basic introduction to the theory and practice of gas chromatographic analysis. It is designed to bring GC users to a more comfortable and capable level of proficiency in instrument operation, optimization, and troubleshooting. Lessons are presented in a visually engaging and accessible manner through narrated on-line video presentations. In full recognition of the difficulty in setting large blocks of uninterrupted time for training; the lessons are presented in short segments that can be easily fit into even the busiest schedules.
• This course presents the basic processes and instrumentation involved with GC analysis.
• The level of material is specifically designed for GC users without extensive formal training.
• Applicable theory is covered to the extent that it helps guide best practices, method optimization and effective troubleshooting.

What will I get from this course?

Upon successful completion of the tutorial, trainees will have increased confidence and skills in using gas chromatographs. They will better understand how GC works, be more competent at detecting and diagnosing problems, and will be better at interpreting the quality of analytical results. In the end, trainees will be more capable and comfortable using gas chromatographs.

Intermediate Gas Chromatography

What does it cover?

The Intermediate Gas Chromatography tutorial is designed for GC users who have a fundamental understanding of how gas chromatography works and who are ready to develop a more in-depth understanding of the technique. As with the ‘Introduction to Gas Chromatography’ course, the ‘Intermediate Gas Chromatography’ course is organized into sections and modules. The sections focus on a given theme and the modular structure underneath exposes students to the information within time-effective sections. The ‘Intermediate Gas Chromatography’ course is a logical next step after completing the ‘Introduction to Gas Chromatography’ course.

What will I get from this is course?

Upon successful completion of the course, trainees will have increased knowledge and skills in development of gas chromatography methods. They will gain an understanding of both qualitative and quantitative methods, as well as expanding their skills to encompass areas such as fast GC, and further developing maintenance and troubleshooting abilities.

LC-MS/MS for Chromatographers

What does it cover?

With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector. You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

What will I get from this course? 

You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise. You will receive a printed copy of the approximately 515 slides included in this course to supplement your note-taking as you view the class. 


Evaluation of performance of Torulaspora delbrueckii, Williopsis saturnus and Kluyveromyces lactis in lychee wine fermentation

Evaluation of performance of Torulaspora delbrueckii Williopsis saturnus and Kluyveromyces lactis in lychee wine fermentation

Food Science Asia 2015 speaker Liu Shao Quan  reports on research conducted for the National University of Singapore. His research centres on the performance of various yeasts in lychee wine fermentation. 


Online Monitoring of Atmospheric Inorganic Gases and Aerosols in The Southeastern and Northwestern USA

Atmospheric aerosols exert a large but uncertain negative (cooling) radiative forcing on regional climate. Their chemical composition affects this climate forcing via aerosol hydroscopicity and consequential cloud interactions, as well as providing clues to the chemical origins of the aerosol. A Metrohm Monitor for AeRosol and Gases in Ambient air (MARGA) instrument was used in summer 2013 as part of the Southeastern Oxidant and Aerosol Study (SOAS), a large field campaign that took place in Centerville, Alabama, USA. A MARGA was used to measure the inorganic composition of aerosols and the key gases (HNO3, NH3, SO2) in equilibrium with each aerosol, enabling elucidation of aerosol composition and inferences about origin. The data from this summer field study are compared to data collected in the Pacific Northwest in the fall of 2013, a comparison which helps to isolate the roles of varying regional SO2 sources, humidity, and temperature on aerosol composition and loading.


Product Showcase

Intelligent Solutions for UHPLC / HPLC Method Development – ACE Method Development Kits

ACE Method Development Kits are designed to maximise selectivity – the key to resolution in chromatography. A complete range of kits for all requirements is available, including kits featuring porous, solid-core and bioanalytical 300Å particle columns. These kits group together the essential column chemistries for method development.


Markes International launches analytical chemistry awareness campaign

Markes International (Llantrisant, UK), in association with Exterion Media (UK) Ltd, is launching a major digital poster campaign to highlight the value of the analytical chemistry industry to society.


Videos demonstrating GC–TOF MS instrument control and data-processing software

Markes International (Llantrisant) has announced the release of a set of six videos explaining the key features of its TOF-DS™ software package.


Polymer-based SPE columns

Biotage announces the addition of new polymer - based SPE columns to their EVOLUTE ® EXPRESS range. EVOLUTE® EXPRESS is a novel family of plates, and now columns, that simplify polymer-based SPE by employing a streamlined three step protocol; Load-Wash-Elute. By using this approach and removing the conditioning and equilibration steps, necessary with traditional bio-analytical SPE procedures, processing and method development times are dramatically reduced without loss of analyte recovery or method robustness.


Latest HPLC Solutions

HPLC Solutions #132: Resources #3: How Do I Validate a Method For...?

This is the third instalment of HPLC Solutions related to resources you should have at your fingertips when you are working in an HPLC laboratory (see also #130, 131). If you work in a regulated industry, such as pharmaceuticals, your HPLC methods likely will need to be validated. If you are using a compendial method from the United States Pharmacopoeia (USP) or European Pharmacopoeia (EP) or other pharmacopoeial method, you may not have to validate the method, but simply demonstrate that it passes system suitability. If the method is modified sufficiently in transfer, it will need some sort of validation.


MS Solutions #28: Quality: The Third Side of the Analytical Success Triangle

As analytical chemists, we spend considerable time thinking about and working with basic scientific principles combined with the technology and products that allow us to solve our analytical problems. However, it is essential for success of any method and for the long-term success of a laboratory operation that our science and technology are supported by a quality system that ensures continuing achievement of our operational goals. We therefore present a triangle concept for analytical success: One side of the triangle shows our need to understand fundamental scientific concepts. A second, and equally sized side, is the ability to apply current technology, applications, software, and techniques in the laboratory. Finally, the base of this equilateral triangle is the quality system. If any one side of the triangle is given inadequate attention or, worse yet, is missing then we cannot hope to achieve overall success for the laboratory operation.



Mass Spectrometry On-demand

Sponsors:   AB SCIEX, Agilent, Bruker, JEOL,, Peak Scientific, Scientific Resources, Shimadzu, Supelco, Waters

Mass Spectrometry is a world-class international conference covering fundamentals, best practice and applications in mass spectrometry and mass spectrometry coupled techniques. The 2013 conference took place in Singapore, offering delegates the chance to learn from some of the world's top experts in the use of mass spectrometry and related techniques. The conference is now available as an on-demand online event providing access to this knowledge base to a wider global audience. On this site you'll find links to presentations given in Singapore which you can listen to on-demand from your own laboratory or office. Learn about the importance of the sample molecule when determining the correct method; discover how to successfully hyphenate chromatographic systems; and what are the pros and cons of the various MS interfaces? Discover how to interpret and understand the data you generate, and understand how MS can be applied to various analytical challenges across market sectors and industries.


Learn more about CORTECS solid-core particle columns, which offer particle sizes ideal for HPLC, UHPLC, and UPLC platforms. These columns are developed for a simple, seamless method transfer with consistent results across different particle sizes and column configurations.

Separation Science is pleased to announce the Chromolith® Technology Microsite. Click on the button below to find details of the educational content available on this site, including technology articles, applications, videos and online resources.