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Separation Science is the leading global portal for fundamentals, best practice and troubleshooting information for users of chromatographic and mass spectrometric techniques. Featuring articles, applications, webinars, workshops and conferences, Separation Science is the industry's 'go to' information resource.





2nd Advances in Forensics & Toxicology eSeminar

Sponsors:   Gerstel

Separation Science, in collaboration with its sponsor Gerstel, has developed an on-demand online eSeminar focusing on advances in forensics & toxicology. This unique event will bring together some of the leading exponents in the forensics and toxicology industries and provide an interactive learning environment for scientists working in these fields.

UV-vis Spectrophotometric Analysis of Water, Food and Beverages

Sponsors:   Merck KGaA

Separation Science, in collaboration with Merck KGaA, has developed an online eSeminar focusing on the use and application of UV-vis spectrophotometry for the analysis of water, food, and beverages. This on-demand event provides scientists with updates on the latest issues faced by individuals working in these challenging analytical fields. This unique on-demand event brings together a selection of technology experts and provide an interactive learning environment and online resource.

Analytical Challenges for Biosimilar Development

Sponsors:   Agilent Technologies

Separation Science, in collaboration with sponsors Agilent Technologies, has developed an online eSeminar focusing on the analytical and bioanalytical challenges posed by the biosimilar development process. This educational resource will provide pharmaceutical and biopharmaceuticals scientists with updates on the latest issues faced by individuals working in this complex field by bringing together some of the leading biosimilar experts and providing an interactive learning environment and online resource..

Protein Analysis Methods for Biopharmaceuticals

 Sponsors:   Tosoh Bioscience GmbH

Separation Science, in collaboration with Tosoh GmbH, has developed and online eSeminar focusing on analytical methods for the analysis of proteins as part of the process of Biopharmaceutical development. This educational resource offers full access to a selection of presentations from leading scientists around the world, offering advances in methods and applications pertaining to this complex analytical field.

Advances in Analytical Methods for Biotherapeutics

 Sponsors:   PSS

Separation Science, in collaboration with PSS, has developed an on-demand eSeminar focusing on analytical and bioanalytical methods for the analysis of biotherapeutic proteins. This educational resource offers full access to a selection of presentations from leading scientists around the world, offering advances in methods and applications pertaining to this complex analytical field.



Improved LC-MS/MS Pesticide Multiresidue Analysis using Triggered MRM and On-Line Dilution 

Presenter:  Dr Katerina Mastovska (Covance Laboratories, Greenfield, Indiana, USA) 
Sponsor:  Agilent Technologies  
Date: 16 February, 2016
Time: 10 am EST / 3 pm GMT / 4 pm CET 

This webinar will discuss development, optimization and performance of a large LC-MS/MS pesticide multiresidue method for separation of about 450 analytes within 10 minutes. The method uses so called Triggered MRM function, which enables acquisition of additional MS/MS transitions for increased confidence in analyte identification. Acetonitrile extracts (prepared using a QuEChERS-based extraction) are injected directly without a need for a dilution with an aqueous buffer/solution prior to the injection because the LC system employs on-line dilution ensuring excellent peak shape of early eluting (more polar) analytes. The method was validated for routine use in multiple food matrices. 


Ionic Liquid Columns, and Multidimensional GC - Advances and Methods

Presenter:  Prof. Philip Marriott (Monash University, Australia)
Sponsor:  Supelco 

Ionic liquid phase columns offer fascinating properties in GC. They are uniformly very polar, and this confers interesting properties to their retention behaviour. For instance, in many cases, the elution temperature of analytes is much lower on IL phases. Thus we obtain reduced elution temperature for fatty acid methyl esters (FAME) as the polarity of the phase increases from IL59, IL60, IL76, IL82, IL100 to IL111.

Non-targeted Screening Workflows for the Analysis of Microcystins by LC/QTOF

Presenter:  Ralph Hindle,  President of Vogon Laboratory Services in Cochrane, Alberta
Sponsor:  Agilent Technologies 

Harmful algae blooms are becoming larger and more frequent around the world. Microcystins are a class of cyanotoxins produced by some algae that pose analytical difficulties in LC/MS methods. Well over 100 variants have been identified in the scientific literature, but only a dozen or so are commercially available. Analysis by triple quadrupole MS will only detect those compounds that are specifically targeted in the method, and may miss either structural isomers or previously unknown toxins.

Transfer of USP Methods from Particulate to Monolithic Columns

Presenter:  Patrik Appelblad, Merck KGaA, Darmstadt, Germany
Sponsor:  Merck KGaA

This webinar will focus on regulated chromatographic analysis and how methods can be transferred from one type of column to another with the same chemistry but with different backbones and physical properties. USP monograph methods, originally developed and validated for use with large particle HPLC columns, have been transferred and partially revalidated for use with monolithic columns.The examples in this webinar will be used to emphasise the new possibilities in changing HPLC methods per chapter 621 in United States Pharmacopeia.

Spice Up Your Life with MS: Workflows for High Throughput Pesticide Analysis by MS/MS in Challenging Matrices such as Spices and Tobacco

Presenter:  Dr Kaushik Banerjee, National Research Centre for Grapes (NRCG), Indian Council of Agricultural Research (ICAR), Pune, India
Sponsor:  Shimadzu

This exclusive Shimadzu Food Safety Webinar will provide an in-depth know-how about the various mass spectrometry (MS/MS) workflows that are needed to conduct high throughput pesticide analysis in challenging matrices. You will learn about the latest state-of-the-art technologies that mass spec leaders are using globally to deal with pesticide analysis in difficult matrices such as spices and tobacco.

Elemental Analysis in Infant Formula, Nutritional Products, and Raw Ingredients by ICP-MS

Presenter: Lawrence Pacquette (Abbott Nutrition, Columbus, Ohio, USA)
Sponsor: Agilent Technologies

In this webinar, Dr Lawrence Pacquette will present how Agilent ICP-MS instruments (7500cx, 7700x and 7900) have not only provided the required sensitivity for ultra-trace elemental analysis, but have also improved the efficiency of Abbott Nutrition’s laboratories for routine testing and helped in producing AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) recognized analytical methods for elemental analysis.

Self-Paced Training


HPLC Basics, Equipment, and Troubleshooting

What does it cover?

“HPLC Basics, Equipment, and Troubleshooting” explains chromatography in practical terms from the ground up.
Here’s what you’ll learn in this online course:
• The basic principles of HPLC
• The five fundamental HPLC modes
• The easy and logical way to adjust experimental variables to get a good separation
• The best approach to use for various chromatographic applications
• How to maximize column life
• Special techniques such as gradient elution, quantitation, and sample pretreatment
• The operating principles of each module in an HPLC system
• Proven techniques for systematic problem-solving and instrument maintenance
• The most effective, timesaving, money saving approaches to preventing common hardware problems and method failures.

What will I get from this is course?

You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient. What you learn will demystify your instrument. You’ll find that all of the perplexing and frustrating problems your experience have simple and logical solutions. And, better yet, you’ll learn that most of these problems are preventable. The techniques you learn from this course will make you more effective at work and save you big headaches! If you’ve never taken a formal course or if you need a refresher, it’s time to learn HPLC the right way. Students tell us that this “extremely practical course” is a must for everyone who uses HPLC.

Advanced HPLC Method Development Using Quality by Design

What does it cover?

The central tenant of Quality by Design (ICH Q8) is that quality cannot be tested into a product – instead it must be designed into the product. When the product is an HPLC method, QbD strategies can guide the development process to result in a standardized method development prcedure, more easily validated methods, and methods that are easier to use and adjust in routine applications.
 “Advanced HPLC Method Development Using Quality by Design” is an intensive online class that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods.

Introduction to Gas Chromatography

What does it cover?

The ‘Introduction to Gas Chromatography’ On-Line Tutorial is basic introduction to the theory and practice of gas chromatographic analysis. It is designed to bring GC users to a more comfortable and capable level of proficiency in instrument operation, optimization, and troubleshooting. Lessons are presented in a visually engaging and accessible manner through narrated on-line video presentations. In full recognition of the difficulty in setting large blocks of uninterrupted time for training; the lessons are presented in short segments that can be easily fit into even the busiest schedules.
• This course presents the basic processes and instrumentation involved with GC analysis.
• The level of material is specifically designed for GC users without extensive formal training.
• Applicable theory is covered to the extent that it helps guide best practices, method optimization and effective troubleshooting.

What will I get from this course?

Upon successful completion of the tutorial, trainees will have increased confidence and skills in using gas chromatographs. They will better understand how GC works, be more competent at detecting and diagnosing problems, and will be better at interpreting the quality of analytical results. In the end, trainees will be more capable and comfortable using gas chromatographs.

Intermediate Gas Chromatography

What does it cover?

The Intermediate Gas Chromatography tutorial is designed for GC users who have a fundamental understanding of how gas chromatography works and who are ready to develop a more in-depth understanding of the technique. As with the ‘Introduction to Gas Chromatography’ course, the ‘Intermediate Gas Chromatography’ course is organized into sections and modules. The sections focus on a given theme and the modular structure underneath exposes students to the information within time-effective sections. The ‘Intermediate Gas Chromatography’ course is a logical next step after completing the ‘Introduction to Gas Chromatography’ course.

What will I get from this is course?

Upon successful completion of the course, trainees will have increased knowledge and skills in development of gas chromatography methods. They will gain an understanding of both qualitative and quantitative methods, as well as expanding their skills to encompass areas such as fast GC, and further developing maintenance and troubleshooting abilities.

LC-MS/MS for Chromatographers

What does it cover?

With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector. You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

What will I get from this course? 

You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise. You will receive a printed copy of the approximately 515 slides included in this course to supplement your note-taking as you view the class. 


IC-ICP/MS Analysis of Iodised X-ray Contrast Media after Ozonation

The use of contrast media in non-invasive diagnostics facilitates targeted imaging of organs and distinction between healthy and unhealthy tissue. One differentiates between contrast media for magnetic resonance imaging (MRI) and X-ray examinations. IC-ICP/MS is ideally suited to monitor degradation products of contrast media after wastewater treatment procedures such as ozonation.


Chromate in Toys, Leather, and Potable Water

Generally speaking, chromate – Cr(VI) – is classified as allergenic, carcinogenic, and extremely toxic and is subject to strict monitoring. Cr(VI) can occur in various concentrations in different areas, e.g., drinking water, toys, or textile and leather products. Metrohm has developed ion chromatographic determination methods for determining Cr(VI) in various concentration ranges (ng/L to mg/L) with inline sample preparation techniques for various matrices. The method can be almost entirely automated. In the following we present the analysis of hexavalent chromium in toys, leather, and drinking water.


Product Showcase

Intelligent Solutions for UHPLC / HPLC Method Development – ACE Method Development Kits

ACE Method Development Kits are designed to maximise selectivity – the key to resolution in chromatography. A complete range of kits for all requirements is available, including kits featuring porous, solid-core and bioanalytical 300Å particle columns. These kits group together the essential column chemistries for method development.


Markes International launches analytical chemistry awareness campaign

Markes International (Llantrisant, UK), in association with Exterion Media (UK) Ltd, is launching a major digital poster campaign to highlight the value of the analytical chemistry industry to society.


Videos demonstrating GC–TOF MS instrument control and data-processing software

Markes International (Llantrisant) has announced the release of a set of six videos explaining the key features of its TOF-DS™ software package.


Polymer-based SPE columns

Biotage announces the addition of new polymer - based SPE columns to their EVOLUTE ® EXPRESS range. EVOLUTE® EXPRESS is a novel family of plates, and now columns, that simplify polymer-based SPE by employing a streamlined three step protocol; Load-Wash-Elute. By using this approach and removing the conditioning and equilibration steps, necessary with traditional bio-analytical SPE procedures, processing and method development times are dramatically reduced without loss of analyte recovery or method robustness.


MIURA GO-xHT - A powerful clean-up system for dioxins and PCBs

Shimadzu is the official distribu­tor of the revolutionary GO-xHT clean-up system from MIURA Institute of Environmental Science. The GO-xHT targets dioxins and PCBs (polychlorinated biphenyl) analyses of food and feedstuff. The combination with Shimadzu’s GCMS-TQ8040 triple quadrupole fulfills the new EU 589/2014 regulation on analysing dioxins and PCBs samples.


Latest HPLC Solutions

HPLC Solutions #133: Resources 4: Where Do I Find More Information About...?

This is the fourth instalment of HPLC Solutions related to resources you should have at your fingertips when you are working in an HPLC laboratory (see also #130, 131,132). In the last discussion (#132) we looked at online resources from the International Conference on Harmonization (ICH) and the United States Food and Drug Administration (FDA) regarding validation of HPLC methods. This time I’d like to mention a few books that I find indispensable in my day-to-day work as a chromatographer. The first three form my trifecta of HPLC books that I refer to most often. 


UV-vis Spectrophotometric Analysis of Water, Food, and Beverages

Separation Science, in collaboration with Merck KGaA, has developed an online eSeminar focusing on the use and application of UV-vis spectrophotometry for the analysis of water, food, and beverages. This on-demand event provide scientists with updates on the latest issues faced by individuals working in these challenging analytical fields. This unique event brings together a selection of technology experts and provide an interactive learning environment and online resource.


Learn more about CORTECS solid-core particle columns, which offer particle sizes ideal for HPLC, UHPLC, and UPLC platforms. These columns are developed for a simple, seamless method transfer with consistent results across different particle sizes and column configurations.

Separation Science is pleased to announce the Chromolith® Technology Microsite. Click on the button below to find details of the educational content available on this site, including technology articles, applications, videos and online resources.