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Welcome to Separation Science


Separation Science is the leading global portal for fundamentals, best practice and troubleshooting information for users of chromatographic and mass spectrometric techniques. Featuring articles, applications, webinars, workshops and conferences, Separation Science is the industry's 'go to' information resource.



Structural Motifs in Primary Oxidation Products of Palmitoyl-arachidonoyl-phosphatidylcholines by LC-MS/MS

Ana Reis from the Mass Spectrometry Center, UI-QOPNA, Chemistry Department, University of Aveiro, Aveiro, Portugal answers questions on her research into structural motifs in primary oxidation products of palmitoyl-arachidonoyl-phosphatidylcholines by LC-MS/MS.


Development of a Rapid Liquid Chromatography Tandem Mass Spectrometry Method for Determination of Trace Caffeine in Surface Water

A rapid LC/MS/MS method for the quantification of caffeine in surface water samples is presented. This method employs large volume injection on a single LC reversed-phase column with detection in ESI positive mode. The method is robust and has the advantage of requiring no extensive sample preparation steps. 



Fully Automated Determination of The Permanganate Index in Accordance with DIN EN ISO 8467

Metrohm presents a method for completely automated determination of the permanganate index according to DIN EN ISO 8467 (DIN 38409-5). The determination of the permanganate index is a standard application performed in almost all water laboratories but keeping the quality of sample preparation consistent — including the time-critical heating of samples — is a challenge.


Determination of Formol Number, Acidity and True Brix in Orange Juice

A method for the determination of formol number (based on recommendations of DIN EN 1133 standard), acidity and true brix of grape and orange juice is presented.


Extraction Cortisol From Human Saliva Using ISOLUTE SLE+ Plates Prior to LC-MS/MS Analysis

This Application Note describes the supported liquid extraction clean-up of Cortisol from saliva prior to quantitative LC-MS/MS analysis. Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress.


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Transferring HPLC & UHPLC Methods (Penang)

17 Nov 2014 09:00 - 17:00

Equatorial Hotel, Penang, Malaysia
Price: US$ 850

A one-day short course about how to transfer reversed-phase high-performance liquid chromatography (RP-HPLC) and ultra-high-performance LC (UHPLC) methods between different instrument platforms.


HPLC and UHPLC Troubleshooting: A Performance Qualification Approach (Penang)

18 Nov 2014 09:00 - 17:00

Equatorial Hotel, Penang, Malaysia
Price: US$ 850

A comprehensive short course in the isolation, correction, and prevention of liquid chromatographic problems. This course is designed for anyone who works with HPLC or UHPLC. No previous experience with HPLC or UHPLC systems is assumed; however, much of the course will appeal to the more experienced worker. This is one of our most popular classes – one that students say is a “must take” for everyone who uses HPLC and UHPLC. Students are encouraged to bring examples of problems they have in the laboratory for discussion in the class.


New Industry Standard HPLCs - The Revolution Has Begun

Shimadzu Corporation announces the global launch of its i-Series of New Industry Standard HPLCs (High Performance Liquid Chromatograph) systems. The i-series improves productivity and promotes an advanced laboratory environment with operation capabilities from built-in touch panel and smart device, thereby enabling a PC-free laboratory.


Shimadzu (Asia Pacific) signs MoU with NERI, sets up NUS-Shimadzu Advanced Facility for Ecoanalytics (NUSAFE)

Singapore-based Shimadzu (Asia Pacific) and National University of Singapore Environmental Research Institute (NERI) have signed a Memorandum of Understanding (MoU) to establish a joint facility to conduct environmental analytical research. The facility will be called NUS-Shimadzu Advanced Facility for Ecoanalytics or NUSAFE


Parker domnick hunter wins the Golden Gas Award at Chicago’s Pittcon

Parker domnick hunter has won a prestigious Golden Gas 2014 Award for its UHP Zero Nitrogen Gas Generator at the Pittcon conference and expo in Chicago.


RESTEK: Rt®-Silica BOND Columns

Restek’s Rt®-Silica BOND columns are robust, versatile, selective PLOT columns that offer excellent performance for the analysis of light hydrocarbons, sulfur gases, and halocarbons above ambient temperature...



HPLC Solutions #122: How to Measure Noise. Part 1

This installment of HPLC Solutions begins a series on different measurements that we make on chromatograms. Measuring the baseline noise generated by an HPLC system may seem like a singularly boring subject. But if you think about it, noise can be a critical factor in the results of our analytical work. It is directly related to the limit of detection and limit of quantification, it can be used to determine the health of the detector, noise is a great diagnostic tool for problems, and can be a limiting factor in the precision of a method.



MS Solutions #28: Quality: The Third Side of the Analytical Success Triangle

As analytical chemists, we spend considerable time thinking about and working with basic scientific principles combined with the technology and products that allow us to solve our analytical problems. However, it is essential for success of any method and for the long-term success of a laboratory operation that our science and technology are supported by a quality system that ensures continuing achievement of our operational goals. We therefore present a triangle concept for analytical success: One side of the triangle shows our need to understand fundamental scientific concepts. A second, and equally sized side, is the ability to apply current technology, applications, software, and techniques in the laboratory. Finally, the base of this equilateral triangle is the quality system. If any one side of the triangle is given inadequate attention or, worse yet, is missing then we cannot hope to achieve overall success for the laboratory operation.



Fast LC Finesse: Maximizing Resolution Using Selectivity

Presented by: Dr Jason Link (Agilent Technologies)
In collaboration with Agilent Technologies


This webinar will go into detail on how a chromatographer can take advantage of new phase chemistries on superficially porous columns, as well as utilize new chemistries optimized for long life at elevated pH for improved resolution.


An Introduction to Chromatographic ICP-MS analysis and its Applications to The Analysis of Environmental Waters

Presenter: Armando Durazo (The University of Arizona, Tucson, Arizona, USA)
In collaboration with Agilent Technologies


This webinar covers an introduction into chromatographic ICP-MS (including ICP-MS source, ionization, etc.), GC-ICPMS for halogenated disinfection byproducts (DBPs) in water extracts, IC-ICPMS for halides and oxyhalides and also iodinated contrast media (ICMs).



Separation Science is pleased to announce the Chromolith® Technology Microsite. Click on the button below to find details of the educational content available on this site, including technology articles, applications, videos and online resources.


19-20 November - Biopolis, Singapore
Two-day price: US$188

This conference will offer an unparalleled series of learning sessions for regular users of chromatography and mass spectrometry techniques. Led by technology experts, delegates will receive an in-depth educational experience covering the fundamentals and best practice of liquid chromatography, gas chromatography, mass spectrometry and associated sample preparation techniques.


Don't miss out on regular learning materials for chromatography, mass spectrometry and related techniques from Separation Science.


Separation Science, in collaboration with LGC, has developed an online eSeminar - Advances in Food Safety Analysis. This free-access eSeminar is now available on-demand and brings together some of the leading exponents in food safety analysis and provide an interactive learning environment for scientists working in these fields.

sponsored by:


Separation Science, in collaboration with Phenomenex, has developed a microsite dedicated to issues surrounding the use of core-shell particle columns. In this section, you'll find articles describing some of the technology behind these columns, applications in small and large molecule separations, as well as a selection of videos and tutorials.


Presentations from Mass Spectrometry 2013 are now available online, providing analysts globally with access to talks given by many of the world's leading authorities on the use and application of MS techniques. For the one-off price of US$79 you can gain access to presentations describing the importance of the sample molecule when determining the correct method; how to successfully hyphenate chromatographic systems;  and the pros and cons of the various MS interfaces? Discover how to interpret and understand the data you generate, and understand how MS can be applied to various analytical challenges across market sectors and industries.

Access is for 6 months and each presentation can be viewed multiple times. For full details on this online resource and how to access it click the 'Read more' button below.



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