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Welcome to Separation Science


Separation Science is the leading global portal for fundamentals, best practice and troubleshooting information for users of chromatographic and mass spectrometric techniques. Featuring articles, applications, webinars, workshops and conferences, Separation Science is the industry's 'go to' information resource.


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Advances in The Development of Organic Polymer Monolithic Columns and Their Applications in Food Analysis—A Review

Pavel Jandera from the Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic provides a review on advances in the development of organic polymer monolithic columns and their applications in food analysis.


GPC/SEC Troubleshooting #9: Baseline Column Detector Peak System -Wavy RI Baseline

Q: The baseline of my refractive index detector, RI, shows maxima and minima and looks wavy. What can be the reason for this? 



Fully Automated Determination of The Permanganate Index in Accordance with DIN EN ISO 8467

Metrohm presents a method for completely automated determination of the permanganate index according to DIN EN ISO 8467 (DIN 38409-5). The determination of the permanganate index is a standard application performed in almost all water laboratories but keeping the quality of sample preparation consistent — including the time-critical heating of samples — is a challenge.


Determination of Formol Number, Acidity and True Brix in Orange Juice

A method for the determination of formol number (based on recommendations of DIN EN 1133 standard), acidity and true brix of grape and orange juice is presented.


Extraction Cortisol From Human Saliva Using ISOLUTE SLE+ Plates Prior to LC-MS/MS Analysis

This Application Note describes the supported liquid extraction clean-up of Cortisol from saliva prior to quantitative LC-MS/MS analysis. Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress.


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Method Development and Optimization for LC/MS/MS

19 May 2014 - 20 May 2014 09:00 - 17:00

Venue: Symbion Science Park, Copenhagen, Denmark  
Price: US$ 1380

This two-day class is ideally suited to analytical chemists and laboratory managers in GLP/GMP regulated industries such as pharmaceuticals, diagnostics, biotechnology, cosmetics, and food. Every chemist developing LC/MS/MS methods in any industry, agency or school must take a systematic approach to ensure valid methods and the quality of their results.  Therefore, this course is for everyone who is presently using LC/MS, may use LC/MS in the future, or who must understand and evaluate data from LC/MS analyses.


Mass Spectral Interpretation

21 May 2014 - 22 May 2014 09:00 - 17:00

Symbion Science Park, Copenhagen, Denmark
Price: US$1,380

The two-day training course on interpretation of mass spectra is designed to develop the fundamental skills necessary to understand fragmentation of ions formed by electron ionization (EI) in GC/MS or by one of the soft ionization techniques employed in LC/MS. Many of the examples are taken from EI mass spectrometry; however, the principles that are learned are applicable to any ionization technique.


Discover the Potential of Label-free High Performance Capillary Electrophoresis through a Process Analytical Technology Case Study - A Revolution in CE Technology

Presenter: Dr Stuart Hassard (deltaDOT Ltd, UK)

Date: 13 May, 2014
Time: 3pm BST / 4pm CET

In collaboration with Horiba Scientific

This webinar will cover how label-free high performance capillary electrophoresis delivers such markedly better data and how can it analyse the whole PAT work-flow? it will also demonstrate data examples from the PAT – raw materials and media through to formulation, fill, finish and release via expression and purification of biopharmaceuticals.


Solid Phase Extraction: Fundamentals and Recent Developments

Presenter: Olga Shimelis (Supelco / Sigma-Aldrich, USA)

In collaboration with Supelco

Date - Thursday May 15th 2014
Time - 8am PDT / 11am EDT / 4pm BST / 5pm CET

The webinar will cover SPE method development with a close look at retention mechanisms and the role of pH in sample preparation. In addition, recent innovations in SPE and methods for current analytes of interest will be presented.



Parker domnick hunter wins the Golden Gas Award at Chicago’s Pittcon

Parker domnick hunter has won a prestigious Golden Gas 2014 Award for its UHP Zero Nitrogen Gas Generator at the Pittcon conference and expo in Chicago.


RESTEK: Rt®-Silica BOND Columns

Restek’s Rt®-Silica BOND columns are robust, versatile, selective PLOT columns that offer excellent performance for the analysis of light hydrocarbons, sulfur gases, and halocarbons above ambient temperature...



Classification of Chamomile Flowers, Essential Oils and Commercial Products Utilizing Chemometrics and the Agilent GC/MSD

Presenter: Professor Jon Parcher (National Center for Natural Products Research, School of Pharmacy, University of Mississippi)


In collaboration with Agilent Technologies

This is a presentation of the application of gas chromatography with mass spectrometric detection (GC/MS) for the analysis and characterization of essential oils with a particular emphasis on chamomile. Analysis of essential oils can be performed by a variety of techniques; however, in this presentation the advantages of GC/MS will be discussed.


A New Dimension in Benchtop GC-MS

Presenters: David Sparkman (University of the Pacific, USA) & Nick Bukowski (Markes International, UK)

In collaboration with: Markes International


This webinar will introduce Select-eV, a revolutionary development in electron ionisation. Until now, soft ionisation by EI hasn't been successfully achieved without a significant sensitivity penalty. Select-eV provides the ability to switch effortlessly between hard and soft ionisation without any loss in sensitivity. This presentation will demonstrate how the underlying benefits of GC-TOFMS along with the ground-breaking capabilities of Select-eV technology have been combined into a compact analytical system, BenchTOF-Select.




HPLC Solutions #121: Autosampler Design, Part 2: Needle-in-Loop

In the last instalment of HPLC Solutions (#120) we looked at one popular autosampler design, the push-to-fill autosampler. Now we’ll turn our attention to the other most popular design, which I call needle-in-loop.



MS Solutions #28: Quality: The Third Side of the Analytical Success Triangle

As analytical chemists, we spend considerable time thinking about and working with basic scientific principles combined with the technology and products that allow us to solve our analytical problems. However, it is essential for success of any method and for the long-term success of a laboratory operation that our science and technology are supported by a quality system that ensures continuing achievement of our operational goals. We therefore present a triangle concept for analytical success: One side of the triangle shows our need to understand fundamental scientific concepts. A second, and equally sized side, is the ability to apply current technology, applications, software, and techniques in the laboratory. Finally, the base of this equilateral triangle is the quality system. If any one side of the triangle is given inadequate attention or, worse yet, is missing then we cannot hope to achieve overall success for the laboratory operation.



Spectroscopy Solutions 2014 eConference (20 & 22 May and 27 & 29 May 2014) is a unique online event, which will focus on key disciplines within atomic and molecular spectroscopy providing attendees with a unique learning opportunity from prominent thought leaders and key educators in these techniques. The eConference will take place over four sessions on four days on consecutive Tuesdays and Thursdays. It will also be available on-demand.



Separation Science, in collaboration with LGC, has developed an online eSeminar - Advances in Food Safety Analysis. This free-access eSeminar is now available on-demand and brings together some of the leading exponents in food safety analysis and provide an interactive learning environment for scientists working in these fields.

sponsored by:


Separation Science, in collaboration with Phenomenex, has developed a microsite dedicated to issues surrounding the use of core-shell particle columns. In this section, you'll find articles describing some of the technology behind these columns, applications in small and large molecule separations, as well as a selection of videos and tutorials.


Presentations from Mass Spectrometry 2013 are now available online, providing analysts globally with access to talks given by many of the world's leading authorities on the use and application of MS techniques. For the one-off price of US$79 you can gain access to presentations describing the importance of the sample molecule when determining the correct method; how to successfully hyphenate chromatographic systems;  and the pros and cons of the various MS interfaces? Discover how to interpret and understand the data you generate, and understand how MS can be applied to various analytical challenges across market sectors and industries.

Access is for 6 months and each presentation can be viewed multiple times. For full details on this online resource and how to access it click the 'Read more' button below.


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