Understanding GPC/SEC – How to deal with pressure problems

by | Articles, Liquid Chromatography

So you're using gel permeation chromatography (GPC) / size exclusion chromatography (SEC) but are experiencing problems with your system pressure. What do you do?

Screenshot2.pngSeparation Science, in collaboration with PSS, has developed an on-going series of tutorials looking at best practice and troubleshooting for GPC users. These tutorials are available free of charge requiring just a simple registration process.

The second tutorial in the series, titled 'Pressure Problems' provides strategies for understanding and rectifying pressure problems arising in your GPC/SEC system, to ensure that your analysis is optimized and that your system does not suffer damage as a result. To begin with you'll learn to identify the underlying problems.

Your standard GPC/SEC system should start off at approximately 95 bar pressure; however, circumstances can results in this pressure level becoming:

  • significantly higher (e.g., 120 bar)
  • significantly lower (e.g., 5 bar)
  • increasingly higher over a loner period of time

This tutorial will look at the reasons for the above changes and provide strategies for correcting all three. It will describe the importance of identifying problems fast and offer best practice advice in terms of calibration, maintenance and cleaning.

DanielaHeld.pngThe presentation is given by Daniela Held. Daniela studied chemistry at the University of Mainz in Germany. Her PhD work was concerned the  synthesis of branched polyelectrolyts and their analysis using light scattering and viscometry detection. Since 2000 she has been working in the PSS solutions department, and she is also responsible for the company’s education programs and customer training.

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