Agarose is obtained from natural sources and contains a very low amount of ionic and hydrophobic groups that could interact with the compound of interest. In contrast,...
A general overview of size-exclusion chromatography and a look at applications in aqueous gel filtration chromatography (GFC) and organic GPC (gel permeation chromatography).
Agarose-based resins from GE Healthcare, biomolecular analysis, broad molecular weight range (Mr 100 to 5000000) from small peptides up to large protein complexes - Superdex 30 Increase,...
Mark your calendar to attend HPLC 2018 Symposium on July 29-August 2, 2018 in Washington DC USA. The HPLC conference will celebrate its 47th anniversary in 2018
SEC - powerful chromatography technique for information on size of biomolecules, analytical applications from basic research/quality control of biotherapeutics
This application note describes the GPC (or equivalently SEC) analysis of branching in polyvinylpyrrolidone (PVP).
Want to understand what the correct criteria for column selection in GPC/SEC should be based on? Why are particle and pore size so important?
Peak shapes and learn how to verify if a GPC / SEC chromatogram is realistic, why some peak shapes occur, why this happens and how to address...
Rectifying pressure problems arising in GPC/SEC system, to ensure analysis is optimized and the system does not suffer damage - identifying underlying problems
Addition of light scattering analysis to SEC provides characterization of molar mass, viscosity, branching, conformation, conjugation, and size of excipients
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